Bioethanol Production from Food Waste Applying the Multienzyme System Produced On-Site by Fusarium oxysporum F3 and Mixed Microbial Cultures

Abstract

Waste management and production of clean and affordable energy are two main challenges that our societies face. Food waste (FW), in particular, can be used as a feedstock for the production of ethanol because of its composition which is rich in cellulose, hemicellulose and starch. However, the cost of the necessary enzymes used to convert FW to ethanol remains an obstacle. The on-site production of the necessary enzymes could be a possible solution. In the present study, the multienzyme production by the fungus Fusarium oxysporum F3 under solid state cultivation using different agroindustrial residues was explored. Maximum amylase, glucoamylase, endoglucanase, b-glucosidase, cellobiohydrolase, xylanase, b-xylosidase and total cellulase titers on wheat bran (WB) were 17.8, 0.1, 65.2, 27.4, 3.5, 221.5, 0.7, 0.052 and 1.5 U/g WB respectively. F. oxysporum was used for the hydrolysis of FW and the subsequent ethanol production. To boost ethanol production, mixed F. oxysporum and S. cerevisiae cultures were also used. Bioethanol production by F. oxysporum monoculture reached 16.3 g/L (productivity 0.17 g/L/h), while that of the mixed culture was 20.6 g/L (productivity 1.0 g/L/h). Supplementation of the mixed culture with glucoamylase resulted in 30.3 g/L ethanol with a volumetric productivity of 1.4 g/L/h.

Optimization of Yeast, Sugar and Nutrient Concentrations for High Ethanol Production Rate Using Industrial Sugar Beet Molasses and Response Surface Methodology

“…These by-products represent a concrete solution to avoid the direct land use impact of using crops for the production of fuels, especially in light of LCA calculations that will determine the carbon impact of biofuels [8]. Recent studies reported the use of various residues such as food waste [9], corncobs [10] and algae waste [11] for the sustainable production of bioethanol. In the case of lignocellulosic biomass, several pre-treatment steps are often required to break down the complex lignin structure and depolymerise the crystalline cellulose to access fermentable sugars [12].…”

Techno-economic analysis of bioethanol production from microwave pretreated kitchen waste

“…It was observed that at 30 °C and pH 6.0 maximum ethanol percentage obtained after 48 h was 14.17%. In a recent study, ethanol yield of 30.8 gL −1 was achieved by using food waste as substrate using in-house developed enzymes for hydrolysis [43]. By comparing the results from the literature, it was concluded that ethanol production efficiency (129.27 g/L) obtained during this work was higher than that compared to other work reported in the literature.…”

“…They achieved an ethanol yield of 107.58 g/kg dry material. In a recent study, Prasoulas et al [43] carried out saccharification of food waste using an enzymatic cocktail from Fusarium oxysporum F3. They obtained a final ethanol yield of 30.3 g/L.…”

Recent advances in bioethanol production from lignocelluloses: a comprehensive review with a focus on enzyme engineering and designer biocatalysts

“…A recent study proved an enhanced bioethanol production of 20.6 g/L with volumetric productivity of 1.0 g/L/h from food waste in a SSF system using the mixed culture of F. oxysporum F3 and S. cerevisiae. The supplementation of glucoamylase into the mixed culture resulted in further enhancement of ethanol production and productivity by 30.3 g/L and 1.4 g/L/h, respectively, and hence, proved the feasibility of on-site production of multienzyme system and bioethanol production from food waste (Prasoulas et al, 2020). Similarly, a pilot-scale continuous tubular reactor (PCTR) technology is expected to achieve a high ethanol yield of 11.0 to 11.3 kg of ethanol per 100 Kg of untreated biomass by overcoming the challenges related to biomass recalcitrance (Pérez-Pimienta et al, 2020).…”