Bioethanol production from Madhuca latifolia L. flowers by a newly isolated strain of Pichia kudriavzevii

Abstract

There is a growing interest worldwide to search new, cheap and non-food biomass source for ethanol production. Bioethanol is a liquid fuel produced from renewable biomass source. In the present study, ethanol production from flowers of Madhuca latifolia trees was investigated by yeast strain isolated from milk whey. The isolated yeast was screened for fermentation ability by carbohydrate fermentation test and subjected to fermentation using M. latifolia flowers as substrate. The isolated yeast strain was identified as Pichia kudriavzevii on the basis of sequencing of D1/D2 domain of rRNA gene through basic local alignment search tool and molecular phylogenetic analysis. Maximum ethanol production, 371 g kg À1 from the flowers was achieved via optimizing operational parameters; a fermentation duration of 48 h, temperature 25 C, and pH 5.0. The effect of nitrogen supplements, enzymatic treatment and co-culture on bioethanol production with the optimized conditions revealed that the optimal condition could produce maximum ethanol without any supplementation or treatment. Co-culturing of P. kudriavzevii strain with Pichia stipitis NCIM 3497 was able to enhance ethanol production. The present study provides baseline information for further scale up of bioethanol production process on industrial level using M. latifolia flowers as a cheap and non-food biomass source.

Box-Behnken design for the optimization of bioethanol production from rice straw and sugarcane bagasse by newly isolatedPichia occidentalisstrain AS.2

“…The inoculated plates were incubated at 30°C for 48 h. Based on microscopic examination and the colony color; different purified single colonies were subcultured in a GYP agar slant and incubated at 30°C for 24 h. After incubation, the pure culture was maintained at 4 °C for further study. 39 Quantitative estimation for bioethanol production by isolated yeast cells was performed after growing the tested cells in GYP broth for 72 h and inoculated with 24 h preculture at 0.2% concentration. Bioethanol production in the fermentation medium was monitored daily, where 1 ml sample was taken and centrifuged for 10 min at 11,000 rpm.…”

“…Generally, different yeasts from many sources can be used as a source of bioethanol, including S. cerevisiae, Lachancea fermentati, Pichia kudriavzevii, Shizosaccharomyces pombe, Candida tropicalis, Zygosaccharomyces rouxii, Saccharomycodes ludwigii, Hanseniaspora guilliermondii, and Wickerhamomyces anomalus isolated from coconut inflorescence sap in Thailand, 69 P. kudriavzevii and Candida tropicalis from pineapple and mango, respectively, 70 Kluyveromyces marxianus from biscuit factories, 40 and P. kudriavzevii from milk whey. 39 Fermentation Initial fermentation using acid-alkali-treated RS and SCB. P. occidentalis strain AS.2 was tested for bioethanol production from the enzymatic hydrolysate of acid-alkali-treated RS and SCB.…”

Saccharification and Fermentation of Cellulolytic Agricultural Biomass to Bioethanol using Locally Isolated Aspergillus niger S48 and Kluyveromyces sp. Y2, respectively

“…Similarly, in another study carried out by Rezania et al [48] their report indicated that bioethanol production optimization was achieved at temperature of 32 °C and higher pH of 6.0 [48]. However, some authors reported optimized pH of 4.5 [49] and lower temperature of 25 °C [50]. The differences observed in the optimized data could be attributed to different fermenting microorganisms used.…”