Bioethanol production from marine biomass alginate by metabolically engineered bacteria

Takeda, Hiroyuki; Yoneyama, Fuminori; Kawai, Shigeyuki; Hashimoto, Wataru; Murata, Kousaku

doi:10.1039/c1ee01236c

Cited by 166 publications

(126 citation statements)

References 22 publications

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“…The engineered microbial platforms for direct production of bioethanol from alginate have been reported. [60][61][62] In conclusion, the enzymes are important tools in a broad spectrum of biological roles and applications. Alginate lyases with different substrate specificities can be used to produce oligosaccharides with various biological functionalities.…”

Section: Functionality and Promising Application Of Alginate Lyasementioning

confidence: 99%

Alginate lyase: Review of major sources and classification, properties, structure-function analysis and applications

2015

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A lginate lyases catalyze the degradation of alginate, a complex copolymer of a-L-guluronate and its C5 epimer b-D-mannuronate. The enzymes have been isolated from various kinds of organisms with different substrate specificities, including algae, marine mollusks, marine and terrestrial bacteria, and some viruses and fungi. With the progress of structural biology, many kinds of alginate lyases of different polysaccharide lyases families have been characterized by obtaining crystal structures, and the catalytic mechanism has also been elucidated. Combined with various studies, we summarized the source, classification and properties of the alginate lyases from different polysaccharide lyases families. The relationship between substrate specificity and protein sequence was also investigated.

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Section: Functionality and Promising Application Of Alginate Lyasementioning

confidence: 99%

Alginate lyase: Review of major sources and classification, properties, structure-function analysis and applications

2015

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“…Technology for the ethanol fermentation of seaweeds began development in the 2000s 9,27 . Yeast and marine bacterium are used for ethanol fermentation 4,7,8,12 . Development of the ethanol fermentation of seaweeds will thus raise expectations for the acetic fermentation of seaweeds, because acetic acid is easily produced from ethanol by acetic acid bacteria 4 .…”

Section: Future Prospects Of Algal Fermentationmentioning

confidence: 99%

Algal Fermentation^|^mdash;The Seed for a New Fermentation Industry of Foods and Related Products

Uchida

Miyoshi

2013

JARQ

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There have been many kinds of fermentation technology and products since ancient times. For example, fermented food items from soybean are common in the East Asian countries of China, Korea and Japan, while those from fish are common in Southeast Asian countries 5 . Despite the long history of fermentation technology, fermented food items produced from algae have yet to be developed (Fig. 1). Many studies were conducted on methane fermentation of seaweeds during the 1970s and 1980s 1, 2, 6 . However, methane fermentation is a technology for supplying energy, not for foods and food production.Macroalgae (macrophytes) can be divided into four groups: brown algae (Phaeophyta), red algae (Rhodophyta), green algae (Chlorophyta), and seagrass (Magnoliophyta). Carbohydrates are the major component of seaweeds and seagrass (ca. 50-70% on a dry basis) 11,29 , containing mostly polysaccharides to construct algal tissue. For example, brown algae contain alginate and fucoidan as major components. Red algae contain galactan (e.g. agar, carrageenan) as a major component. Green algae and seagrasses contain cellulose and hemicellulose as major components. These major algal polysaccharides are known to be unfavorable substrates for fermentation. This may be one of the reasons why algal fermentation technology has yet to be developed. However, it was recently reported that seaweed could be used as a substrate for lactic acid and ethanol fermentation, provided that the algal tissue was saccharified with cellulase enzymes. This finding opened the possibility of obtaining foods and related items from algal fermentation 17,18,19 . This manuscript reviews past studies on the lactic acid fermentation of algae 17,22 . It also refers to other kinds of algal fermentation that are now being developed, such AbstractMany kinds of fermented products are now being consumed as food and dietary items, although those produced from algae have yet to be developed. A recent observation that seaweed could be used as a substrate for lactic acid fermentation opened the possibility of obtaining such products as foods, diets and fertilizers by algal fermentation. This manuscript reviews past studies on the lactic acid fermentation of algae. Both macroalgae (seaweeds) and microalgae can be used as the materials for lactic acid fermentation, as successful fermentation has been observed regarding all the seaweed species tested to date. Saccharification by cellulase treatment is considered a significant element for inducing algal fermentation. The addition of a starter culture of lactic acid bacteria and salt also promotes successful fermentation. A wide range of Lactobacillus species can be used for inducing algal fermentation, with Lactobacillus brevis, Lactobacillus casei and Lactobacillus plantarum in particular showing a superior ability to dominate in seaweed fermentation cultures. A starter culture of halophilic lactic acid bacteria that is now being developed will make it possible to prepare algal fermented products containing a high (>10%) salt con...

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“…Brown seaweeds, a traditional and plentiful mariculture product in East Asia, make up a large proportion of the total biomass of algae and synthesize a wide variety of compounds, such as alginate, fucoidan, laminarin, and mannitol (16). Among these compounds, alginate has been assumed to be a potential source for bioethanol production (17)(18)(19).…”

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confidence: 99%

Isolation and Complete Genome Sequence of Algibacter alginolytica sp. nov., a Novel Seaweed-Degrading Bacteroidetes Bacterium with Diverse Putative Polysaccharide Utilization Loci

Sun

Zhang

et al. 2016

Appl Environ Microbiol

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eThe members of the phylum Bacteroidetes are recognized as some of the most important specialists for the degradation of polysaccharides. However, in contrast to research on Bacteroidetes in the human gut, research on polysaccharide degradation by marine Bacteroidetes is still rare. The genus Algibacter belongs to the Flavobacteriaceae family of the Bacteroidetes, and most species in this genus are isolated from or near the habitat of algae, indicating a preference for the complex polysaccharides of algae. In this work, a novel brown-seaweed-degrading strain designated HZ22 was isolated from the surface of a brown seaweed (Laminaria japonica). On the basis of its physiological, chemotaxonomic, and genotypic characteristics, it is proposed that strain HZ22 represents a novel species in the genus Algibacter with the proposed name Algibacter alginolytica sp. nov. The genome of strain HZ22, the type strain of this species, harbors 3,371 coding sequences (CDSs) and 255 carbohydrate-active enzymes (CAZymes), including 104 glycoside hydrolases (GHs) and 18 polysaccharide lyases (PLs); this appears to be the highest proportion of CAZymes (ϳ7.5%) among the reported strains in the class Flavobacteria. Seventeen polysaccharide utilization loci (PUL) are predicted to be specific for marine polysaccharides, especially algal polysaccharides from red, green, and brown seaweeds. In particular, PUL N is predicted to be specific for alginate. Taking these findings together with the results of assays of crude alginate lyases, we prove that strain HZ22 T can completely degrade alginate. This work reveals that strain HZ22 T has good potential for the degradation of algal polysaccharides and that the structure and related mechanism of PUL in strain HZ22T are worth further research. Members of the phylum Bacteroidetes, formerly also known as the Cytophaga-Flavobacteria-Bacteroides cluster, constitute one of the major groups of marine heterotrophic bacterioplankton (1, 2). They have been found in various marine habitats, including coastal sediments (3), coastal waters (4, 5), hydrothermal vents (6, 7), and open ocean waters (8-10). In previous studies, marine Bacteroidetes have been reported as important contributors to the utilization of biopolymers such as polysaccharides and proteins (2,(11)(12)(13)(14). As a result, marine Bacteroidetes are assumed to play an important role in the degradation of algae. Marine phytoplankton have been estimated to be responsible for about 50% of global net primary production (15). Polysaccharides constitute a substantial fraction of the primary production from marine phytoplankton. Algae can be an important source of polysaccharides. Brown seaweeds, a traditional and plentiful mariculture product in East Asia, make up a large proportion of the total biomass of algae and synthesize a wide variety of compounds, such as alginate, fucoidan, laminarin, and mannitol (16). Among these compounds, alginate has been assumed to be a potential source for bioethanol production (17-19).The genus Algibacter belongs...

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Bioethanol production from marine biomass alginate by metabolically engineered bacteria

Cited by 166 publications

References 22 publications

Alginate lyase: Review of major sources and classification, properties, structure-function analysis and applications

Alginate lyase: Review of major sources and classification, properties, structure-function analysis and applications

Algal Fermentation^|^mdash;The Seed for a New Fermentation Industry of Foods and Related Products

Isolation and Complete Genome Sequence of Algibacter alginolytica sp. nov., a Novel Seaweed-Degrading Bacteroidetes Bacterium with Diverse Putative Polysaccharide Utilization Loci

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