Bioethanol Production from Sugarcane Bagasse by a Novel Brazilian Pentose Fermenting YeastScheffersomyces shehataeUFMG-HM 52.2: Evaluation of Fermentation Medium

Abstract

Bioconversion of hemicellulosic sugars into second generation (2G) ethanol plays a pivotal role in the overall success of biorefineries. In this study, ethanol production performance of a novel xylose-fermenting yeast,Scheffersomyces shehataeUFMG-HM 52.2, was evaluated under batch fermentation conditions using sugarcane bagasse (SB) hemicellulosic hydrolysate as carbon source. Dilute acid hydrolysis of SB was performed to obtain sugarcane bagasse hemicellulosic hydrolysate (SBHH). It was concentrated, detoxified, and supplemented with nutrients in different formulations to prepare the fermentation medium to the yeast evaluation performance.S. shehataeUFMG-HM 52.2 (isolated from Brazilian Atlantic rain forest ecosystem) was used in fermentations carried out in Erlenmeyer flasks maintained in a rotator shaker at 30°C and 200 rpm for 72 h. The use of a fermentation medium composed of SBHH supplemented with 5 g/L ammonium sulfate, 3 g/L yeast extract, and 3 g/L malt extract resulted in 0.38 g/g of ethanol yield and 0.19 g L.h of volumetric productivity after 48 h of incubation time.

Production of bioethanol in sugarcane bagasse hemicellulosic hydrolysate byScheffersomyces parashehatae,Scheffersomyces illinoinensisandSpathaspora arborariaeisolated from Brazilian ecosystems

“…Its ethanol yields and productivities were 0·125 g g −1 and 0·069 g l −1 h −1 respectively (Antunes et al . ); 0·38 g g −1 and 0·189 g l −1 h −1 respectively (Chandel et al . ); 0·31 g g −1 and 0·12 g l −1 h −1 respectively (Antunes et al .…”

“…parashehatae UFMG-CM-Y507 and Sc. illinoinensis UFMG-CM-Y510 have previously been evaluated for their ability to ferment lignocellulosic hydrolysates (da Cunha-Pereira et al 2011;Ferreira et al 2011;Chandel et al 2013Chandel et al , 2014Martiniano et al 2013;Antunes et al 2014Antunes et al , 2016Hickert et al 2014;Duss an et al 2016). In brief, Sp.…”

Harnessing the potential of bio‐ethanol production from lignocellulosic biomass in Nigeria – a review

“…Antunes et al . worked on ethanol production from sugarcane bagasse using novel xylose‐fermenting yeast, named Scheffersomyces shehatae UFMG‐HM 52.2.…”

“…Antunes et al 26 worked on ethanol production from sugarcane bagasse using novel xylose-fermenting yeast, countries. The use of corn cobs to produce bioethanol comes with some challenges and to surmount these challenges, some factors, including but not limited to the mode of harvesting, crop handling, storage/preservation methods and conversion methods, should be developed further.…”

Bioethanol fermentation from sugarcane bagasse using ragi tape

“…However, there are some yeasts able to ferment xylose to ethanol or other products, such as Pachysolen tannophilus Boidin and Adzet, 1957 and Pichia stipitis Pignal, 1967 [9,10,5], Candida tropicalis (Castellani) Berkhout, 1923 and Candida shehatae Kurtzman, 1990 [11,5,12] Urbina, 2012 [13]. It is now considered possible to hydrolyze xylan enzymatically to simple reducing sugar, and use it to produce ethanol [14]. Xylose is not fermented by S. cerevisiae.…”