Biofabrication of a Functional Tubular Construct from Tissue Spheroids Using Magnetoacoustic Levitational Directed Assembly

Parfenov, Vladislav A.; Koudan, Elizaveta V.; Krokhmal, A. A.; Annenkova, Elena A.; Petrov, Stanislav V.; Pereira, Frederico D. A. S.; Каралкин, П. А.; Nezhurina, Elizaveta K.; Gryadunova, A. A.; Буланова, Елена А.; Sapozhnikov, Oleg A.; Tsysar, S. A.; Liu, Kaizheng; Oosterwijk, Egbert; Beuningen, H. van; Kraan, P. van der; Granneman, S.J.C.; Engelkamp, Hans; Christianen, Peter C. M.; Kasyanov, Vladimir; Khesuani, Yusef D.; Mironov, Vladimir

doi:10.1002/adhm.202000721

Cited by 23 publications

(17 citation statements)

References 50 publications

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“…8 That result emphasizes that different responses are obtained with the same agents when cells are cultured in 2D and 3D, and it confirms that the 3D microenvironment provides better resistance against toxic substances. 46,47 It is worth mentioning that recently the influence of Gx on cell viability was also investigated in 3D spheroids, and it was reported 48,49 that 50 mM Gx provides around 87% viability, which also correlates well with our findings.…”

Section: Effect Of Paramagnetic Agent On Tunable 3dsupporting

confidence: 86%

Fabrication of Tunable 3D Cellular Structures in High Volume Using Magnetic Levitation Guided Assembly

Onbas

Yıldız

2021

ACS Appl. Bio Mater.

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Tunable and reproducible size with high circularity is an important limitation to obtain three-dimensional (3D) cellular structures and spheroids in scaffold free tissue engineering approaches. Here, we present a facile methodology based on magnetic levitation (MagLev) to fabricate 3D cellular structures rapidly and easily in high-volume and low magnetic field. In this study, 3D cellular structures were fabricated using magnetic levitation directed assembly where cells are suspended and self-assembled by contactless magnetic manipulation in the presence of a paramagnetic agent. The effect of cell seeding density, culture time, and paramagnetic agent concentration on the formation of 3D cellular structures was evaluated for NIH/3T3 mouse fibroblast cells. In addition, magnetic levitation guided cellular assembly and 3D tumor spheroid formation was examined for five different cancer cell lines: MCF7 (human epithelial breast adenocarcinoma), MDA-MB-231 (human epithelial breast adenocarcinoma), SH-SY5Y (human bone-marrow neuroblastoma), PC-12 (rat adrenal gland pheochromocytoma), and HeLa (human epithelial cervix adenocarcinoma). Moreover, formation of a 3D coculture model was successfully observed by using MDA-MB-231 dsRED and MDA-MB-231 GFP cells. Taken together, these results indicate that the developed MagLev setup provides an easy and efficient way to fabricate 3D cellular structures and may be a feasible alternative to conventional methodologies for cellular/multicellular studies.

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Section: Effect Of Paramagnetic Agent On Tunable 3dsupporting

confidence: 86%

Fabrication of Tunable 3D Cellular Structures in High Volume Using Magnetic Levitation Guided Assembly

Onbas

Yıldız

2021

ACS Appl. Bio Mater.

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“…The organ-on-a-chip and spheroid culture systems are evolving to better mimic human pathophysiology, which is being enhanced by the integration of sensors and robotics [1][2][3][4][5][6][7][8]. Among MPS, spheroids are emerging with the potential to mimic human tissues in a 3D shape and function [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Gravity-Based Flow Efficient Perfusion Culture System for Spheroids Mimicking Liver Inflammation

Kim,

Asif,

Chethikkattuveli Salih

et al. 2021

Biomedicines

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The spheroid culture system provides an efficient method to emulate organ-specific pathophysiology, overcoming the traditional two-dimensional (2D) cell culture limitations. The intervention of microfluidics in the spheroid culture platform has the potential to enhance the capacity of in vitro microphysiological tissues for disease modeling. Conventionally, spheroid culture is carried out in static conditions, making the media nutrient-deficient around the spheroid periphery. The current approach tries to enhance the capacity of the spheroid culture platform by integrating the perfusion channel for dynamic culture conditions. A pro-inflammatory hepatic model was emulated using a coculture of HepG2 cell line, fibroblasts, and endothelial cells for validating the spheroid culture plate with a perfusable channel across the spheroid well. Enhanced proliferation and metabolic capacity of the microphysiological model were observed and further validated by metabolic assays. A comparative analysis of static and dynamic conditions validated the advantage of spheroid culture with dynamic media flow. Hepatic spheroids were found to have improved proliferation in dynamic flow conditions as compared to the static culture platform. The perfusable culture system for spheroids is more physiologically relevant as compared to the static spheroid culture system for disease and drug analysis.

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“…Interestingly, Parfenov et al . recently reported a hybrid magnetoacoustic bioassembly method that allowed rapid assembly of 3D tissue construction from spheroids within a medium, with a relatively low concentration of paramagnetic agent (gadolinium salt)[ 178 ]. Harnessing the strength from both magnetic waves and acoustic sounds, this method not only circumvents a challenge from a potentially adverse effect stemming from the paramagnetic agent, but also imparts more flexibility on the assembled structure.…”

Section: Progress In Establishing 3d Tumor Models Via 3d Bioprintingmentioning

confidence: 99%

Using Spheroids as Building Blocks Towards 3D Bioprinting of Tumor Microenvironment

Zhuang¹,

Chiang²,

Fernanda³

et al. 2024

IJB

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Cancer still ranks as a leading cause of mortality worldwide. Although considerable efforts have been dedicated to anticancer therapeutics, progress is still slow, partially due to the absence of robust prediction models. Multicellular tumor spheroids, as a major three-dimensional (3D) culture model exhibiting features of avascular tumors, gained great popularity in pathophysiological studies and high throughput drug screening. However, limited control over cellular and structural organization is still the key challenge in achieving in vivo like tissue microenvironment. 3D bioprinting has made great strides toward tissue/organ mimicry, due to its outstanding spatial control through combining both cells and materials, scalability, and reproducibility. Prospectively, harnessing the power from both 3D bioprinting and multicellular spheroids would likely generate more faithful tumor models and advance our understanding on the mechanism of tumor progression. In this review, the emerging concept on using spheroids as a building block in 3D bioprinting for tumor modeling is illustrated. We begin by describing the context of the tumor microenvironment, followed by an introduction of various methodologies for tumor spheroid formation, with their specific merits and drawbacks. Thereafter, we present an overview of existing 3D printed tumor models using spheroids as a focus. We provide a compilation of the contemporary literature sources and summarize the overall advancements in technology and possibilities of using spheroids as building blocks in 3D printed tissue modeling, with a particular emphasis on tumor models. Future outlooks about the wonderous advancements of integrated 3D spheroidal printing conclude this review.

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Biofabrication of a Functional Tubular Construct from Tissue Spheroids Using Magnetoacoustic Levitational Directed Assembly

Cited by 23 publications

References 50 publications

Fabrication of Tunable 3D Cellular Structures in High Volume Using Magnetic Levitation Guided Assembly

Fabrication of Tunable 3D Cellular Structures in High Volume Using Magnetic Levitation Guided Assembly

Gravity-Based Flow Efficient Perfusion Culture System for Spheroids Mimicking Liver Inflammation

Using Spheroids as Building Blocks Towards 3D Bioprinting of Tumor Microenvironment

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