Biofilm amount estimation by fluorescein diacetate

Rosa, Salvatore De; Sconza, F.; Volterra, L

doi:10.1016/s0043-1354(98)00019-0

Cited by 21 publications

(7 citation statements)

References 30 publications

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“…Fluorescein diacetate (FDA) assay modified from Battin (1997) and De Rosa et al (1998): Hydrolysis of the FDA molecule estimates general enzyme activity (ability to degrade organic matter) of microorganisms in a sample. Aliquots (200 µL) of biofilm extracted as described previously (phosphate buffer, pH 7.2) were shaken for 1 min at 400 rpm in a black microtitre plate.…”

Section: Methodsmentioning

confidence: 99%

Groundwater Biofilm Dynamics Grown In Situ Along a Nutrient Gradient

Williamson¹,

Leonard

et al. 2012

Groundwater

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This paper describes the in situ response of groundwater biofilms in an alluvial gravel aquifer system on the Canterbury Plains, New Zealand. Biofilms were developed on aquifer gravel, encased in fine mesh bags and suspended in protective columns in monitoring wells for at least 20 weeks. Four sites were selected in the same groundwater system where previous analyses indicated a gradient of increasing nitrate down the hydraulic gradient from Sites 1 to 4. Measurements during the current study classified the groundwater as oligotrophic. Biofilm responses to the nutrient gradients were assessed using bioassays, with biomass determined using protein and cellular and nucleic acid staining and biofilm activity using enzyme assays for lipid, carbohydrate, phosphate metabolism, and cell viability. In general, biofilm activity decreased as nitrate levels increased from Sites 1 to 4, with the opposite relationship for carbon and phosphorus concentrations. These results showed that the groundwater system supported biofilm growth and that the upper catchment supported efficient and productive biofilms (high ratio of activity per unit biomass).

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Section: Methodsmentioning

confidence: 99%

Groundwater Biofilm Dynamics Grown In Situ Along a Nutrient Gradient

Williamson¹,

Leonard

et al. 2012

Groundwater

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“…2 did not affect the activity of the biofilm and dissolved oxygen in the system. The colour of the biofilm could indicate the type and characteristics of the microbial fauna of the biofilm (Antonie 1987;Sirianuntapiboon and Tondee 2000;Sirianuntapiboon 2000;Characklis 1990;Rosa 1998). The grey-brown and dark brown colour of the biofilm indicated that the types of microorganisms were facultative or anaerobic microorganisms (Antonie 1987;Sirianuntapiboon and Tondee 2000;Sirianuntapiboon 2000).…”

Section: Discussionmentioning

confidence: 99%

Efficiency of Packed Cage RBC System for Treatment of Wastewater Containing Surfactants

Sirianuntapiboon

Srikattanaprom

2003

Water Quality Research Journal

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The chemical oxygen demand (COD) and five-day biological oxygen demand (BOD5) removal efficiencies of a packed cage rotating biological contactor (RBC) system under a hydraulic retention time (HRT) of 16 h declined from 93.90% to 78.58% and 78.61% and 96.27% to 84.83% and 87.93%, respectively, with an increase of sodium dodecyl sulphate (SDS) and triton X-100 loading from 0.00 to 1.25 mg/m 2 ⋅d. Reactor no. 1 of the system changed to anaerobic when the system was operated under an HRT of 8 h with surfactant loading of up to 2.55 mg/m 2 ⋅d. However, reactor no. 2 of the system was aerobic and the dissolved oxygen was higher than 3.00 mg/L. The study showed that this type of packed cage RBC system could be applied for treating the cosmetic industry wastewater with surfactant loading of up to 2.55 mg/m 2 ⋅d under an HRT of 8 h.

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“…FDA hydrolysis assay was modified from Battin (1997) and De Rosa et al (1998). Aliquots (200 μL) of biofilm extracted as described previously (Williamson et al, 2012) in phosphate buffer (pH 7.2) were shaken for 1 min at 400 rpm in a black microtitre plate.…”

Section: Fluorescein Diacetatementioning

confidence: 99%