Biofilm and Cell Adhesion Strength on Dental Implant Surfaces via the Laser Spallation Technique

Boyd, James D.; Miller, Craig S.; Grady, Martha E.

doi:10.1101/2019.12.11.873240

Cited by 5 publications

(14 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Surface roughness at order of microns increased the effective surface area of cell adhesion. It could be concluded that cells on the laser-modified surface are immobilized, and the cell deposition/adhesion rates increase as a consequence of cells anchored in morphology features especially in sub-micro- and micro-channels [ 49 , 50 , 51 ].…”

Section: Resultsmentioning

confidence: 99%

Response of NIH 3T3 Fibroblast Cells on Laser-Induced Periodic Surface Structures on a 15×(Ti/Zr)/Si Multilayer System

et al. 2020

View full text Add to dashboard Cite

Ultrafast laser processing with the formation of periodic surface nanostructures on the 15×(Ti/Zr)/Si multilayers is studied in order to the improve cell response. A novel nanocomposite structure in the form of 15×(Ti/Zr)/Si multilayer thin films, with satisfying mechanical properties and moderate biocompatibility, was deposited by ion sputtering on an Si substrate. The multilayer 15×(Ti/Zr)/Si thin films were modified by femtosecond laser pulses in air to induce the following modifications: (i) mixing of components inside of the multilayer structures, (ii) the formation of an ultrathin oxide layer at the surfaces, and (iii) surface nano-texturing with the creation of laser-induced periodic surface structure (LIPSS). The focus of this study was an examination of the novel Ti/Zr multilayer thin films in order to create a surface texture with suitable composition and structure for cell integration. Using the SEM and confocal microscopies of the laser-modified Ti/Zr surfaces with seeded cell culture (NIH 3T3 fibroblasts), it was found that cell adhesion and growth depend on the surface composition and morphological patterns. These results indicated a good proliferation of cells after two and four days with some tendency of the cell orientation along the LIPSSs.

show abstract

Section: Resultsmentioning

confidence: 99%

Response of NIH 3T3 Fibroblast Cells on Laser-Induced Periodic Surface Structures on a 15×(Ti/Zr)/Si Multilayer System

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The laser spallation technique is a quantitative adhesion strength measurement method that applies stress wave loading, causing separation of a film of interest from the substrate at a critical stress value [18][19][20]. Because the stress load is applied indirectly to the material interface through impingement of a pulsed laser on the back side of the specimen, laser spallation is valuable for characterizing bulk adhesion properties of a wide range of materials.…”

Section: Introductionmentioning

confidence: 99%

Sucrose-mediated formation and adhesion strength of Streptococcus mutans biofilms on titanium

Waldman

Butera

Boyd

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

Biofilms consist of bacterial cells surrounded by a matrix of extracellular polymeric substance (EPS), which protects the colony from many countermeasures, including antibiotic treatments. Biofilm EPS composition is affected by environmental factors. In the oral cavity, the presence of sucrose affects the growth of Streptococcus mutans that produce acids, eroding enamel and forming dental caries. Biofilm formation on dental implants commonly leads to severe infections and failure of the implant. This work determines the effect of sucrose concentration on biofilm EPS formation and adhesion of Streptococcus mutans, a common oral colonizer. Bacterial biofilms are grown with varying concentrations of sucrose on titanium substrates simulating dental implant material. Strategies for measuring adhesion for films such as peel tests are inadequate for biofilms, which have low cohesive strength and will fall apart when tensile loading is applied directly. The laser spallation technique is used to apply stress wave loading to the biofilm, causing the biofilm to delaminate at a critical tensile stress threshold. Biofilm formation and EPS structures are visualized at high magnification with scanning electron microscopy (SEM). Biofilm substrate coverage and adhesion strength of biofilms initially increase with increasing sucrose concentration, but then decrease as sucrose concentration continues to increase. For biofilms grown with non-zero concentrations of sucrose, S. mutans adhesion to the substrate is higher than the adhesion of osteoblast-like cells to the same substrates. These results suggest sucrose-mediated adhesion and formation on titanium of S. mutans biofilms may outcompete osteoblasts during osseointegration, which could explain higher rates of peri-implant disease associated with high sugar diets.

show abstract

“…[1][2][3][4][5] A key factor that often modulates adhesion strength within these systems is an increase in surface roughness. [5][6][7] While some researchers report an increase in adhesion strength 5,6 as assessed by laser spallation, others report a decrease. 7 During these experiments, the effect of surface roughness on stress wave generation is often omitted.…”

mentioning

confidence: 99%

“…This work is motivated by an adhesion study of cells and biofilms on dental implant-mimicking titanium surfaces. 6,12 Therefore, the chosen surfaces in this work represent smooth and rough commercial titanium dental implants. Scanning electron microscopy (SEM) images of smooth and rough substrates are shown in Fig.…”

mentioning

confidence: 99%

“…This result means that the substrate stress profiles obtained on smooth samples can be used for calibration with the spallation data obtained on the roughened titanium, overcoming the challenge to collect interferometric data on a rough substrate. 6 Because of this finding, further research can easily be conducted into adhesion of biological films onto medical implant mimicking surfaces without the need for cumbersome calibration protocols. Similarly, TBCs, as stated previously, which often benefit from surface roughened metal surfaces to increase adhesion, can be calibrated effectively, along with countless film/substrate systems, which benefit from micrometer-sized surface roughness.…”

mentioning

confidence: 99%

See 1 more Smart Citation

The effect of surface roughness on laser-induced stress wave propagation

Boyd

Grady

2020

Applied Physics Letters

Self Cite

View full text Add to dashboard Cite

We investigate laser-induced acoustic wave propagation through smooth and roughened titanium-coated glass substrates. Acoustic waves are generated in a controlled manner via the laser spallation technique. Surface displacements are measured during stress wave loading by the alignment of a Michelson-type interferometer. A reflective coverslip panel facilitates capture of surface displacements during loading of as-received smooth and roughened specimens. Through interferometric experiments, we extract the substrate stress profile at each laser fluence (energy per area). The shape and amplitude of the substrate stress profile are analyzed at each laser fluence. Peak substrate stress is averaged and compared between smooth specimens with the reflective panel and rough specimens with the reflective panel. The reflective panel is necessary because the surface roughness of the rough specimens precludes in situ interferometry. Through these experiments, we determine that the surface roughness employed has no significant effect on substrate stress propagation and smooth substrates are an appropriate surrogate to determine stress wave loading amplitude of roughened surfaces less than 1.2 lm average roughness (Ra). No significant difference was observed when comparing the average peak amplitude and loading slope in the stress wave profile for the smooth and rough configurations at each fluence.

show abstract

Biofilm and Cell Adhesion Strength on Dental Implant Surfaces via the Laser Spallation Technique

Cited by 5 publications

References 47 publications

Response of NIH 3T3 Fibroblast Cells on Laser-Induced Periodic Surface Structures on a 15×(Ti/Zr)/Si Multilayer System

Response of NIH 3T3 Fibroblast Cells on Laser-Induced Periodic Surface Structures on a 15×(Ti/Zr)/Si Multilayer System

Sucrose-mediated formation and adhesion strength of Streptococcus mutans biofilms on titanium

The effect of surface roughness on laser-induced stress wave propagation

Contact Info

Product

Resources

About