Biofilm and hemagglutinin formation: a Hallmark for drug resistant uropathogenic Escherichia coli

Hagos, Dawit Gebreegziabiher; Mezgebo, Tadele Araya; Berhane, Samuel; Medhanyie, Araya Abraha

doi:10.1186/s13104-019-4382-1

Cited by 5 publications

(2 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, our results revealed that not only were the majority of ESBLs-producing and MDR isolates susceptible to imipenem, meropenem, and fosfomycin but susceptibility to amikacin, cefoxitin, and nitrofurantoin was also considerable. This finding is consistent with some previous reports from Iran and other countries [29,31]. As carbapenems are β-lactam antibiotics with high antibacterial activity, they are commonly used as first-line treatments for infections caused by ESBLs-producing Enterobacterales.…”

Section: Discussionsupporting

confidence: 92%

See 1 more Smart Citation

Evaluation of adhesin genes and risk factors associated with urinary tract infections by drug-resistant uropathogenic Escherichia coli in North of Iran

Sheikhi,

Amini,

Alidoust

et al. 2024

J Infect Dev Ctries

View full text Add to dashboard Cite

Background: Uropathogenic Escherichia coli (UPEC) isolates, have a wide variety of virulence factors to promote colonization and survival in the urinary tract. This study aimed to evaluate adhesin genes, biofilm formation ability, antibiotic resistance profiles of UPEC strains, and the related risk factors in patients with UTIs caused by drug-resistant UPEC. Methodology: A total of 105 UPEC isolates were evaluated for biofilm formation using 96-well microtiter plates, the presence of adhesin genes by PCR assay and the antimicrobial susceptibility pattern using the disk diffusion method. Demographic and clinical characteristics of patients were investigated to identify predisposing factors for drug-resistant isolates. Results: Out of 105 UPEC isolates, 84.8% were positive for biofilm formation. Biofilm-producing isolates exhibited a significantly higher prevalence of fimH, kpsMTII, csgA, afa/draBC, and pap adhesin genes compared to non-biofilm-producing strains (p < 0.05). The results also revealed that 52.4% of the isolates were ESBL-producing, and 84.8% were multidrug-resistant (MDR). Further analysis of antibiotic susceptibility among ESBL-producing strains showed the highest resistance rates to ampicillin, ciprofloxacin, and trimethoprim-sulfamethoxazole. Conversely, the highest susceptibility, in addition to carbapenems, was observed for fosfomycin, amikacin, cefoxitin, and nitrofurantoin. We identified hypertension as a potential risk factor for infection with ESBL-producing UPEC strains. Conclusions: Our results revealed a significant rate of drug resistance among UPEC isolates obtained from UTIs in our region. This underscores the importance of monitoring the empirical use of antibiotics and identifying specific risk factors in our geographical area to guide the selection of appropriate empirical treatment for UTIs.

show abstract

Section: Discussionsupporting

confidence: 92%

“…Consistent with the previous studies in Iran and other countries, in our results, 52.4 and 84.8% of UPEC isolates belonged to ESBLs-producing and MDR strains, respectively [27][28][29][30][31]. The prevalence of MDR isolates was higher than the pooled prevalence of MDR UPEC with 49.4% [32] and 65.8% [33] in Iran.…”

Section: Discussionsupporting

confidence: 91%