Biofilm-associated heat resistance of Bacillus cereus spores in vitro and in a food model, Cheonggukjang jjigae

Pawluk, Alixander Mattay; Kim, Dabin; Jin, Young Hun; Jeong, Kwangcheol C.; Mah, Jae-Hyung

doi:10.1016/j.ijfoodmicro.2021.109505

Cited by 10 publications

(3 citation statements)

References 60 publications

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“…Biofilms are communities formed on the surfaces of objects by attached bacteria. Biofilms can protect microorganisms from ultraviolet radiation [ 15 ], extreme temperatures [ 16 ], extreme pH, high salinity, and high pressure [ 17 , 18 ]. The agr locus consists of four genes (agrBDCA) that encode the components of the QS system found in several Gram-positive bacteria [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

Integrative Multiomics Analysis of the Heat Stress Response of Enterococcus faecium

Wang

Qiao

et al. 2023

Biomolecules

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A continuous heat-adaptation test was conducted for one Enterococcus faecium (E. faecium) strain wild-type (WT) RS047 to obtain a high-temperature-resistant strain. After domestication, the strain was screened with a significantly higher ability of heat resistance. which is named RS047-wl. Then a multi-omics analysis of transcriptomics and metabolomics was used to analyze the mechanism of the heat resistance of the mutant. A total of 98 differentially expressed genes (DEGs) and 115 differential metabolites covering multiple metabolic processes were detected in the mutant, which indicated that the tolerance of heat resistance was regulated by multiple mechanisms. The changes in AgrB, AgrC, and AgrA gene expressions were involved in quorum-sensing (QS) system pathways, which regulate biofilm formation. Second, highly soluble osmotic substances such as putrescine, spermidine, glycine betaine (GB), and trehalose-6P were accumulated for the membrane transport system. Third, organic acids metabolism and purine metabolism were down-regulated. The findings can provide target genes for subsequent genetic modification of E. faecium, and provide indications for screening heat-resistant bacteria, so as to improve the heat-resistant ability of E. faecium for production.

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Section: Discussionmentioning

confidence: 99%

Integrative Multiomics Analysis of the Heat Stress Response of Enterococcus faecium

Wang

Qiao

et al. 2023

Biomolecules

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“…Spores, one of the special structures of Bacillus spp., can withstand adverse environments and make later virulence release possible [ 34 ]. Biofilms increase the resistance of spores or vegetative cells, which can protect B. cereus to different degrees [ 35 ]. In total, 49 low-redundancy protein-encoding genes were obtained by Swiss-Prot database annotation to determine exactly which genes regulate spore and biofilm formation.…”

Section: Discussionmentioning

confidence: 99%

Functional Analysis of Stress Resistance of Bacillus cereus SCL10 Strain Based on Whole-Genome Sequencing

Mao,

Yang,

Lin

et al. 2024

Microorganisms

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A Gram-positive, rod-shaped, aerobic, motile, and spore-forming bacterium, designated SCL10, was isolated from Acaudina molpadioides exposure to Co-60 radiation. In this study, whole-genome sequencing was performed to identify the strain as Bacillus cereus and functional characterization, with a focus on stress resistance. The genome of the B. cereus SCL10 strain was sequenced and assembled, revealing a size of 4,979,182 bp and 5167 coding genes. The genes involved in biological functions were annotated by using the GO, COG, KEGG, NR, and Swiss-Prot databases. The results showed that genes related to alkyl hydroperoxide reductase (ahpC, ahpF), DNA-binding proteins from starved cells (dps), spore and biofilm formation (spoVG, spo0A, gerP), cold shock-like protein (cspC, cspE), ATP-dependent chaperone (clpB), and photolyase, small, acid-soluble spore protein (SASP) and DNA repair protein (recA, radD) could explain the stress resistance. These findings suggest that antioxidant activity, sporulation, biofilm formation, and DNA protection may be considered as the main resistance mechanisms under exposure to radiation in the B. cereus SCL10 strain.

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“…Bacillus bacteria are ubiquitous in the environment and they can slightly contaminate all foodstuffs, starting with the raw materials. Their spores are able to adhere to any work surface within the food industries [19,20], enhancing their resistance to adverse environmental conditions if incorporated in the biofilms that many of the B. cereus strains are able to produce [21,22]. B. cereus causes two forms of food poisoning in humans via two different types of toxins.…”

Section: The Production Process and Its Influence On The Soup Microbiotamentioning

confidence: 99%

The Growth Potential of Bacillus cereus in Ready-to-Reheat Vegetable Soups

Alberghini,

Fabbian,

Ferioli

et al. 2023

Hygiene

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Bacillus cereus (hereafter, B. cereus) poisoning often arises from the consumption of Ready-To-Reheat vegetable soups in which an intensive growth of the vegetative cells of B. cereus take place. The market for these soups is increasing significantly worldwide. For the producer it is important to determine if soups can promote the growth of B. cereus, by calculating its growth potential. We can achieve this goal by carrying out an efficient challenge test. In our study we have designed and performed a challenge test in three batches of an emmer (Triticum monococcum) and vegetable soup that undergo a second pasteurization treatment after packaging. We found out that under refrigeration conditions B. cereus is unable to multiply in the soup, instead, under conditions of thermal abuse, B. cereus can grow during 90 days of shelf life with a growth potential of 0.82 logarithms. It is essential to keep the entire production phase under control using effective GMP (Good Manufacturing Practices) and GHP (Good Hygiene Practices) measures, to ensure that the freshly produced soups contain low loads of the spores of B. cereus. In this way, the vegetative cells born from the germination of the spores cannot reach the infectious dose necessary to induce the food poisoning.

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Biofilm-associated heat resistance of Bacillus cereus spores in vitro and in a food model, Cheonggukjang jjigae

Cited by 10 publications

References 60 publications

Integrative Multiomics Analysis of the Heat Stress Response of Enterococcus faecium

Integrative Multiomics Analysis of the Heat Stress Response of Enterococcus faecium

Functional Analysis of Stress Resistance of Bacillus cereus SCL10 Strain Based on Whole-Genome Sequencing

The Growth Potential of Bacillus cereus in Ready-to-Reheat Vegetable Soups

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