Biofilm cue for larval settlement in Hydroides elegans (Polychaeta): is contact necessary?

Hadfield, Michael G.; Nedved, Brian T.; Wilbur, Sean L.; Koehl, M. A. R.

doi:10.1007/s00227-014-2529-0

Cited by 38 publications

(26 citation statements)

References 47 publications

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“…This knowledge combined with the non-specificity of amino acids strongly indicates they are unlikely to be the true inducer produced by C. lytica. Certainly, amino acids cannot explain the selective choices of settlement locations observed in the wild or the requirement for physical contact between the larvae and the biofilm (23). For these reasons, we concluded that none of the low-activity metabolites from the bioassay guided fractionations were involved in natural metamorphic induction.…”

Section: Discussionmentioning

confidence: 83%

“…As is the case for a vast majority of marine invertebrates, an external stimulant induces an extremely rapid series of morphogenetic events that quickly transform the swimming larva of H. elegans into a bottom-living, tube-dwelling juvenile that will grow rapidly into a reproductive adult (20). Larvae of H. elegans typically do not settle in the absence of a biofilm, and this response follows direct contact with surface biofilms (14,(20)(21)(22)(23)(24)(25). Investigations of the complex biofilm communities that induce settlement and metamorphosis in H. elegans and the cues that they produce must take place in the context of this ecology.…”

Section: Main Text Introductionmentioning

confidence: 99%

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Bacterial lipopolysaccharide induces settlement and metamorphosis in a marine larva

Freckelton

Nedved

Cai

et al. 2019

Preprint

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Bacterially induced metamorphosis has been observed in marine invertebrate larvae from nearly every major marine phylum. Despite the widespread nature of this phenomenon the mechanism of this process remains poorly understood. The serpulid polychaete Hydroides elegans is a well-established model system for understanding bacteria-mediated larval development. A broad range of bacterial biofilm species elicit larval metamorphosis in this species via at least two mechanisms, including outer membrane vesicles and phage-tail bacteriocins. Here, we investigated the interaction between larvae of H. elegans and the inductive bacterium Cellulophaga lytica, which produces an abundance of OMVs but not phage-tail bacteriocins. We asked whether the OMVs of C. lytica induce larval settlement due to cell membrane components or through delivery of specific cargo. Employing a biochemical structure-function approach, and with a strong ecological focus, the cells and outer membrane vesicles produced by C. lytica were interrogated to determine the structure of the inductive molecule. Here we report that lipopolysaccharide is the inductive molecule produced by C. lytica that induces larvae of H. elegans to metamorphose. The widespread prevalence of LPS and its associated taxonomic and structural variability suggest that it could be a broadly employed cue to bacterially induced larval settlement of marine invertebrates.Significance StatementWhenever new surfaces are created in the sea, they are quickly populated by dense communities of invertebrate animals, whose establishment and maintenance requires site-specific settlement of larvae from the plankton. Although it is recognized that larvae selectively settle in sites where they can metamorphose and thrive and that the bacteria residing in biofilms on these surfaces are important suppliers of cues, the nature of the cues used to identify the ‘right places’ has remained enigmatic. In this paper, we reveal that lipopolysaccharide (LPS) molecules from a marine Gram-negative bacterium are the cuing molecules for a tropical marine worm and demonstrate the likelihood that LPS provides the variation necessary to be the settlement cue for the majority of bottom-living invertebrate animals.

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Section: Discussionmentioning

confidence: 83%

Section: Main Text Introductionmentioning

confidence: 99%

Bacterial lipopolysaccharide induces settlement and metamorphosis in a marine larva

Freckelton

Nedved

Cai

et al. 2019

Preprint

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“…(Lee et al, 2014) Increasing bacterial load of different genera in biofilms seems to be responsible for the primary colonization of submerged surfaces by the tubeworm Hydroides elegans (Olivier et al, 2000;Harder et al, 2002;Lau et al, 2002;Huang and Hadfield, 2003;Shikuma and Hadfield, 2005). Contact between larvae and biofilm appears to be necessary for this interaction to occur (Hadfield et al, 2014), and there is also the mediation of AHLs's such N-hexanoyl-Lhomoserine lactone (Fig. 4-5), N-dodecanoyl-L-homoserine lactone ( Fig.…”

Section: Marine Biofilms and Chemical Cuesmentioning

confidence: 99%

Marine biofilms: diversity of communities and of chemical cues

Antunes

Leão

Vasconcelos

2018

Environ Microbiol Rep

102

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Summary Surfaces immersed in seawater are rapidly colonized by various microorganisms, resulting in the formation of heterogenic marine biofilms. These communities are known to influence the settlement of algae spores and invertebrate larvae, triggering a succession of fouling events, with significant environmental and economic impacts. This review covers recent research regarding the differences in composition of biofilms isolated from different artificial surface types and the influence of environmental factors on their formation. One particular phenomenon – bacterial quorum sensing (QS) – allows bacteria to coordinate swarming, biofilm formation among other phenomena. Some other marine biofilm chemical cues are believed to modulate the settlement and the succession of macrofouling organisms, and they are also reviewed here. Finally, since the formation of a marine biofilm is considered to be an initial, QS‐dependent step in the development of marine fouling events, QS inhibition is discussed on its potential as a tool for antibiofouling control in marine settings.

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“…Fouling biofilm is considered as an important factor for the formation of subsequent fouling communities and has been extensively studied (Qian et al, 2007;Ganesan et al, 2010;Hadfield et al, 2014). However, very few reports addressed the relationship between diatoms and bacteria in biofilm (Bruckner et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

The possible role of bacterial signal molecules N-acyl homoserine lactones in the formation of diatom-biofilm ( Cylindrotheca sp.)

Yang

Fang

Chen

et al. 2016

Marine Pollution Bulletin

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Biofilm cue for larval settlement in Hydroides elegans (Polychaeta): is contact necessary?

Cited by 38 publications

References 47 publications

Bacterial lipopolysaccharide induces settlement and metamorphosis in a marine larva

Bacterial lipopolysaccharide induces settlement and metamorphosis in a marine larva

Marine biofilms: diversity of communities and of chemical cues

The possible role of bacterial signal molecules N-acyl homoserine lactones in the formation of diatom-biofilm ( Cylindrotheca sp.)

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