Biofilm Detection With Hematoxylin-Eosin Staining

Abstract

Hematoxylin-eosin staining of surgical specimens is a reliable and available method for the detection of bacterial biofilm in chronic infectious disease.

Optical coherence tomography for biofilm detection in chronic rhinosinusitis with nasal polyposis

“…According to recently published data of Hochstim et al, we have also conWrmed that the wide availability of H.E. and Gram staining through routine histopathology laboratories makes this a reliable method for detection of bioWlms in the clinical practice [15,16].…”

“…Nevertheless, bioWlms can be detected by diYcult, expensive, and time-consuming protocols of scanning electron microscopy (SEM), transmission electron microscopy (TEM), confocal laser scanning microscopy (CLSM), and recently by microbe-speciWc Xuorescent in situ hybridization (FISH) [8,13,14]. Hochstim et al [15] have reported a robust method for bioWlm detection that was based on the conventional hematoxylin-eosin (H.E.) staining of fresh surgical specimens obtained from patients with CRSwNP.…”

Critical review on biofilm methods

“…Hematoxylin and eosin (H&E), periodic acid-Schiff (PAS), and Brown and Brenn Gram staining have been recently proposed as practical, cheap and reliable methods for detection of bacterial biofilms in different infection foci (Akiyama et al, 2003;Bulut et al, 2014;Davis et al, 2008;Hochstim et al, 2010;Oates et al, 2014;Toth et al, 2011;Winther et al, 2009;Zhang et al, 2009). The detection of biofilm by these practical and cost-effective staining methods has been used for a quantitative assessment of biofilm biomass (Bakke et al, 2001;Bulut et al, 2014;de Carvalho & da Fonseca, 2007) and could have a significant prognostic value (Hong et al, 2014).…”

Current in vitro assay to determine bacterial biofilm formation of clinical isolates

“…Moreover this technique provides highly specific identification of different bacterial species but semiquantitative results. 28 Another technique to visualize biofilm's presence directly from clinical sampel is reported by Hochstim et al 35 by using hematoxylin-eosin (HE) staining. It is a reliable and available method for the detection of bacterial biofilm in chronic infectious disease.…”

“…In particular, the wide availability of HE staining of surgical specimens through clinical pathology laboratories makes this a highly practical method for detecting biofilm in clinical practice. 35 This assay can not identifiy and differentiate the bacterial composition of biofilm and just present an early screening of biofilm-related infection in clinical sampel.…”