Biofilm eradication ability of phage cocktail against Listeria monocytogenes biofilms formed on food contact materials and effect on virulence-related genes and biofilm structure

Byun, Kye-Hwan; Han, Sang Ha; Choi, Min Woo; Kim, Byoung-Hu; Park, Si Hong; Ha, Sang‐Do

doi:10.1016/j.foodres.2022.111367

Cited by 22 publications

(3 citation statements)

References 50 publications

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“…While numerous studies have emphasized the potential of phages as alternatives to antibiotics and biocides for biofilm treatment, they fall short of fully detailing the dynamics of virus-host interactions. Existing research [44][45][46] typically relies on measurements at daily or hourly intervals, involving labor-intensive cell counts. This approach, constrained by its fixed-time nature, often overlooks critical information in the rapidly evolving host-virus relationship, particularly for fast-metabolizing human pathogenic bacteria.…”

Section: Bacterial Development Monitoringmentioning

confidence: 99%

Tracking of Bacteriophage Predation on Pseudomonas aeruginosa Using a New Radiofrequency Biofilm Sensor

Longo,

Lelchat,

Le Baut

et al. 2024

Sensors

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Confronting the challenge of biofilm resistance and widespread antimicrobial resistance (AMR), this study emphasizes the need for innovative monitoring methods and explores the potential of bacteriophages against bacterial biofilms. Traditional methods, like optical density (OD) measurements and confocal microscopy, crucial in studying biofilm–virus interactions, often lack real-time monitoring and early detection capabilities, especially for biofilm formation and low bacterial concentrations. Addressing these gaps, we developed a new real-time, label-free radiofrequency sensor for monitoring bacteria and biofilm growth. The sensor, an open-ended coaxial probe, offers enhanced monitoring of bacterial development stages. Tested on a biological model of bacteria and bacteriophages, our results indicate the limitations of traditional OD measurements, influenced by factors like sedimented cell fragments and biofilm formation on well walls. While confocal microscopy provides detailed 3D biofilm architecture, its real-time monitoring application is limited. Our novel approach using radio frequency measurements (300 MHz) overcomes these shortcomings. It facilitates a finer analysis of the dynamic interaction between bacterial populations and phages, detecting real-time subtle changes. This method reveals distinct phases and breakpoints in biofilm formation and virion interaction not captured by conventional techniques. This study underscores the sensor’s potential in detecting irregular viral activity and assessing the efficacy of anti-biofilm treatments, contributing significantly to the understanding of biofilm dynamics. This research is vital in developing effective monitoring tools, guiding therapeutic strategies, and combating AMR.

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Section: Bacterial Development Monitoringmentioning

confidence: 99%

Tracking of Bacteriophage Predation on Pseudomonas aeruginosa Using a New Radiofrequency Biofilm Sensor

Longo,

Lelchat,

Le Baut

et al. 2024

Sensors

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“…Most significant was the low-percentage-of-lysis characterization of these phages, as that was selected to reduce the chances of resistance development and minimize the potential harm to important normal flora [ 25 ]. Overall, however, bacteriophage studies point out that the success of bacteriophage treatment of biofilms is highly variable depending on factors, such as strains, temperature, multiplicity of infection (MOI), biofilm content or age, and the surfaces or foods involved [ 26 ]. For instance, a study using bacteriophage cocktails (LMPC01+02+03) to target and destroy L. monocytogenes foodborne biofilms demonstrated that, with these particular phages, biofilm maturity had a greater impact on the antibiofilm activities than temperature or surface material, whereas with other phage and biofilms, different factors can become the most significant [ 26 ].…”

Section: Bacteria Viruses and Fungi To Control Biofilmsmentioning

confidence: 99%

“…Overall, however, bacteriophage studies point out that the success of bacteriophage treatment of biofilms is highly variable depending on factors, such as strains, temperature, multiplicity of infection (MOI), biofilm content or age, and the surfaces or foods involved [ 26 ]. For instance, a study using bacteriophage cocktails (LMPC01+02+03) to target and destroy L. monocytogenes foodborne biofilms demonstrated that, with these particular phages, biofilm maturity had a greater impact on the antibiofilm activities than temperature or surface material, whereas with other phage and biofilms, different factors can become the most significant [ 26 ]. Despite the variability and potential limiting factors, bacteriophage therapy is ultimately a very valuable tool in microbial control [ 27 ].…”

Section: Bacteria Viruses and Fungi To Control Biofilmsmentioning

confidence: 99%

The Use of Natural Methods to Control Foodborne Biofilms

Esposito

Turku

2022

Pathogens

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Biofilms are large aggregates of various species of bacteria or other microorganisms tightly attached to surfaces through an intricate extracellular matrix. These complex microbial communities present quite the challenge in the food processing industry, as conditions such as raw meats and diverse food product content in contact with workers, drains, machinery, and ventilation systems, make for prime circumstances for contamination. Adding to the challenge is the highly resistant nature of these biofilm growths and the need to keep in mind that any antimicrobials utilized in these situations risk health implications with human consumption of the products that are being processed in these locations. For that reason, the ideal means of sanitizing areas of foodborne biofilms would be natural means. Herein, we review a series of innovative natural methods of targeting foodborne biofilms, including bacteriocins, bacteriophages, fungi, phytochemicals, plant extracts, essential oils, gaseous and aqueous control, photocatalysis, enzymatic treatments, and ultrasound mechanisms.

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Survival strategies of Listeria monocytogenes to environmental hostile stress: biofilm formation and stress responses

Byun,

Kim

2023

Food Sci Biotechnol

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Biofilm eradication ability of phage cocktail against Listeria monocytogenes biofilms formed on food contact materials and effect on virulence-related genes and biofilm structure

Cited by 22 publications

References 50 publications

Tracking of Bacteriophage Predation on Pseudomonas aeruginosa Using a New Radiofrequency Biofilm Sensor

Tracking of Bacteriophage Predation on Pseudomonas aeruginosa Using a New Radiofrequency Biofilm Sensor

The Use of Natural Methods to Control Foodborne Biofilms

Survival strategies of Listeria monocytogenes to environmental hostile stress: biofilm formation and stress responses

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