Biofilm formation and interspecies interactions in mixed cultures of thermo-acidophilic archaea Acidianus spp. and Sulfolobus metallicus

Castro, Camila; Zhang, Ruiyong; Liu, Jing; Bellenberg, Sören; Neu, Thomas R.; Donati, Edgardo R.; Sand, Wolfgang; Vera, Mario

doi:10.1016/j.resmic.2016.06.005

Cited by 18 publications

(13 citation statements)

References 22 publications

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“…They assume interactions between these acidophiles, because they found that different combinations of these acidophiles had either positive or negative effects on the dissolution of pyrite. Castro et al (Castro et al, 2016) showed that Sulfolobus metallicus and Acidianus copahuensis negatively influenced each other during initial attachment and pyrite dissolution, and physical contact between the two species was detected. Maulani et al (2016) found that pre-established biofilms of Leptospirillum ferriphilum inhibited the initial attachment to pyrite by cells of Ferroplasma acidiphilum and did not promote pyrite leaching by F. acidiphilum.…”

Section: Introductionmentioning

confidence: 99%

Interactions Between Cells of Sulfobacillus thermosulfidooxidans and Leptospirillum ferriphilum During Pyrite Bioleaching

Zhu

et al. 2020

Front. Microbiol.

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Sulfobacillus and Leptospirillum occur frequently in leaching systems. Here we investigated the effects of cells of L. ferriphilum on biofilm formation and leaching performance by S. thermosulfidooxidans. The effects were caused by the presence of L. ferriphilum or an addition of pyrite leach liquor from L. ferriphilum. Data show that the number of attached S. thermosulfidooxidans on pyrite increases, if the pyrite had been pre-colonized by living biofilms of L. ferriphilum, while it decreases if the pre-colonized biofilms had been inactivated. Coaggregation between S. thermosulfidooxidans and L. ferriphilum occurs during the dual-species biofilm formation, but different effects on bioleaching were noted, if the preculture of L. ferriphilum had been different. If L. ferriphilum had been pre-colonized on a pyrite, significantly negative effect was shown. However, if the two species were simultaneously inoculated into a sterile leaching system, the bioleaching efficiency was better than that of a pure culture of S. thermosulfidooxidans. The effect might be related to a metabolic preference of S. thermosulfidooxidans. If S. thermosulfidooxidans performed leaching in a filtered pyrite leachate from L. ferriphilum, the cells preferred to oxidize RISCs instead of ferrous ion and the number of attached cells decreased compared with the control. This study gives an indication that in a short-term multi-species leaching system the role of S. thermosufidooxidans may be related to the time of its introduction.

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Section: Introductionmentioning

confidence: 99%

Interactions Between Cells of Sulfobacillus thermosulfidooxidans and Leptospirillum ferriphilum During Pyrite Bioleaching

Zhu

et al. 2020

Front. Microbiol.

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“…Also, these microbial footprints were detected in the interaction of Acidianus spp . and Sulfolobus metallicus with pyrite [ 21 ]. These results reveal that detachment processes play an important role during biofilm formation of archaeal species on mineral surfaces such as pyrite and chalcopyrite.…”

Section: Resultsmentioning

confidence: 99%

“…Several studies have provided significant knowledge about these processes for mesophilic microorganisms [ 19 , 20 ]. Recently, studies on the adhesion to mineral substrates, EPS production, and biofilm formation by thermoacidophilic archaea such as the genera Acidianus and Sulfolobus have been reported [ 21 , 22 , 23 , 24 ]. However, only a few of them refer to chalcopyrite.…”

Section: Introductionmentioning

confidence: 99%

Importance of Initial Interfacial Steps during Chalcopyrite Bioleaching by a Thermoacidophilic Archaeon

2020

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Studies of thermophilic microorganisms have shown that they have a considerable biotechnological potential due to their optimum growth and metabolism at high temperatures. Thermophilic archaea have unique characteristics with important biotechnological applications; many of these species could be used in bioleaching processes to recover valuable metals from mineral ores. Particularly, bioleaching at high temperatures using thermoacidophilic microorganisms can greatly improve metal solubilization from refractory mineral species such as chalcopyrite (CuFeS2), one of the most abundant and widespread copper-bearing minerals. Interfacial processes such as early cell adhesion, biofilm development, and the formation of passive layers on the mineral surface play important roles in the initial steps of bioleaching processes. The present work focused on the investigation of different bioleaching conditions using the thermoacidophilic archaeon Acidianus copahuensis DSM 29038 to elucidate which steps are pivotal during the chalcopyrite bioleaching. Fluorescent in situ hybridization (FISH) and confocal laser scanning microscopy (CLSM) were used to visualize the microorganism–mineral interaction. Results showed that up to 85% of copper recovery from chalcopyrite could be achieved using A. copahuensis. Improvements in these yields are intimately related to an early contact between cells and the mineral surface. On the other hand, surface coverage by inactivated cells as well as precipitates significantly reduced copper recoveries.

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“…S. metallicus is a thermophilic obligate autotrophic archaeon which grows by oxidizing ferrous iron, S 0 , and reductive inorganic sulfur compounds at temperatures between 65 and 80 • C [20,21]. It was activated on a rotary shaker at 65 • C in 500 mL Erlenmeyer flasks with 200 mL basal medium with 1% chalcopyrite as the sole energy source [22]. For microscopy studies, cells were cultivated in the basal medium 88 (M88) with chalcopyrite slices.…”

Section: Strain and Culture Mediummentioning

confidence: 99%

“…The initial adsorbed cells on the mineral surface were measured in carbon atom amount (at %). The formation of biofilm was visualized by staining with DAPI (4 6-diamidino-2-phenylindole) that was dissolved in phosphate buffered saline (PBS) to a concentration of 10 µg/mL for 10 min [22]. For each EDS result, at least six spots were used for statistical analysis.…”

Section: Adsorption Behaviors Analysismentioning

confidence: 99%

The Evidence of Decisive Effect of Both Surface Microstructure and Speciation of Chalcopyrite on Attachment Behaviors of Extreme Thermoacidophile Sulfolobus metallicus

et al. 2018

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The effect of the surface microstructure and chemical speciation of chalcopyrite on the attachment behaviors of thermoacidophilic archaeon Sulfolobus metallicus was evaluated for the first time by using integrated techniques including epifluorescence microscopy (EFM) and sulfur K-edge X-ray absorption near edge structure (S K-edge XANES) spectroscopy, as well as scanning electron microscopy with energy dispersive spectrometry (SEM/EDS) and Fourier transform infrared (FT-IR) spectroscopy. In order to obtain the specific surface, the chalcopyrite slices were electrochemically oxidized at 0.87 V and reduced at −0.54 V, respectively. The EFM analysis showed that the quantity of cells attaching on the mineral surface increased with time, and the biofilm formed faster on the electrochemically treated slices than on the untreated ones. The SEM-EDS analysis indicated that the deficiency in energy substrate elemental sulfur (S 0) in the specific microsize of local defect sites was disadvantageous to the initial attachment of cells. The XANES and FT-IR data suggested that the elemental sulfur (S 0) could be in favor of initial attachment, and surface jarosites inhibited the adsorption and growth of S. metallicus. These results demonstrated that not only the surface microstructure but also the chemical speciation defined the initial attachment behaviors and biofilm growth of the extreme thermophilic archaeon S. metallicus.

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Biofilm formation and interspecies interactions in mixed cultures of thermo-acidophilic archaea Acidianus spp. and Sulfolobus metallicus

Cited by 18 publications

References 22 publications

Interactions Between Cells of Sulfobacillus thermosulfidooxidans and Leptospirillum ferriphilum During Pyrite Bioleaching

Interactions Between Cells of Sulfobacillus thermosulfidooxidans and Leptospirillum ferriphilum During Pyrite Bioleaching

Importance of Initial Interfacial Steps during Chalcopyrite Bioleaching by a Thermoacidophilic Archaeon

The Evidence of Decisive Effect of Both Surface Microstructure and Speciation of Chalcopyrite on Attachment Behaviors of Extreme Thermoacidophile Sulfolobus metallicus

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