Biofilm formation and molecular analysis of intercellular adhesion gene cluster (icaABCD) among Staphylococcus aureus strains isolated from children with adenoiditis

Ghaioumy, Rasoul; Tabatabaeifar, Fatemehalsadat; Mozafarinia, Karamat; Mianroodi, Aliasghar Arabi; Isaei, Elham; Morones‐Ramírez, José Rubén; Afshari, Setareh Agha Kuchak; Kalantar-Neyestanaki, Davood

doi:10.18502/ijm.v13i4.6969

Cited by 8 publications

(3 citation statements)

References 24 publications

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“…The icaABCD operation in isolates of S. aureus was associated with the formation of biofilms. Ghaioumy and col. (2021) [ 72 ] reported that from 46 clinical samples, 41% expressed icaA and icaD (6.3% and 59.4%, respectively), although icaC and icaB were not detected, and 100% of the isolates of S. aureus were biofilm producers. In our study, the expression of the genes icaA , icaB , icaC , and icaD (51.3%, 32.4%, 24.3%, and 43.24%, respectively), were detected in S. aureus , while in SOSA strains, the genes icaA and icaB (44.1% and 32.5%) were detected, and all the strains were biofilm producers.…”

Section: Discussionmentioning

confidence: 99%

Detection of mecA Genes in Hospital-Acquired MRSA and SOSA Strains Associated with Biofilm Formation

González-Vázquez,

Córdova-Espinoza,

Escamilla-Gutiérrez

et al. 2024

Pathogens

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Methicillin-resistant (MR) Staphylococcus aureus (SA) and others, except for Staphylococcus aureus (SOSA), are common in healthcare-associated infections. SOSA encompass largely coagulase-negative staphylococci, including coagulase-positive staphylococcal species. Biofilm formation is encoded by the icaADBC operon and is involved in virulence. mecA encodes an additional penicillin-binding protein (PBP), PBP2a, that avoids the arrival of β-lactams at the target, found in the staphylococcal cassette chromosome mec (SCCmec). This work aims to detect mecA, the bap gene, the icaADBC operon, and types of SCCmec associated to biofilm in MRSA and SOSA strains. A total of 46% (37/80) of the strains were S. aureus, 44% (35/80) S. epidermidis, 5% (4/80) S. haemolyticus, 2.5% (2/80) S. hominis, 1.25% (1/80) S. intermedius, and 1.25% (1/80) S. saprophyticus. A total of 85% were MR, of which 95.5% showed mecA and 86.7% β-lactamase producers; thus, Staphylococcus may have more than one resistance mechanism. Healthcare-associated infection strains codified type I-III genes of SCCmec; types IV and V were associated to community-acquired strains (CA). Type II prevailed in MRSA mecA strains and type II and III in MRSOSA (methicillin-resistant staphylococci other than Staphylococcus aureus). The operon icaADBC was found in 24% of SA and 14% of SOSA; probably the arrangement of the operon, fork formation, and mutations influenced the variation. Methicillin resistance was mainly mediated by the mecA gene; however, there may be other mechanisms that also participate, since biofilm production is related to genes of the icaADBC operon and methicillin resistance was not associated with biofilm production. Therefore, it is necessary to strengthen surveillance to prevent the spread of these outbreaks both in the nosocomial environment and in the community.

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Section: Discussionmentioning

confidence: 99%

Detection of mecA Genes in Hospital-Acquired MRSA and SOSA Strains Associated with Biofilm Formation

González-Vázquez,

Córdova-Espinoza,

Escamilla-Gutiérrez

et al. 2024

Pathogens

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“…The ica locus ( ica ADBC) encodes the enzyme N -acetyl-glucosaminyltranferase, which participates in the production of polysaccharide intercellular adhesion (PIA), facilitating the biofilm formation of S. aureus ( Cue et al, 2012 ). A previous study has reported differences in the detection rate of ica ADBC in the biofilm formation process of S. aureus, and the absence of a certain ica ADBC may not necessarily affect the biofilm formation ( Ghaioumy et al, 2021 ). This also explains the slightly higher detection rates of ica A and ica D (both 100%) than those of ica B and ica C (both 96.7%) in this study.…”

Section: Discussionmentioning

confidence: 99%

Molecular typing and characterization of Staphylococcus aureus isolates from burn wound infections in Fujian, China

Hong,

Zhou,

Dai

et al. 2023

Front. Microbiol.

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BackgroundStaphylococcus aureus (S. aureus) is the most common causative agent of burn wound infection, that often leads to high morbidity and mortality. However, there is not enough knowledge about the molecular epidemiology and antimicrobial susceptibility of S. aureus isolates from burn wound infections in Fujian, China.MethodsBetween 2016 and 2021, 90 S. aureus isolates were collected from burn wound infections in Fujian, China, including 59 methicillin-resistant (MRSA) strains and 31 methicillin-susceptible (MSSA) strains. These were investigated for molecular characteristics, virulence genes, biofilms, and antimicrobial susceptibility. All the isolates were genotyped by multilocus sequence typing (MLST), spa typing, agr typing, and SCCmec typing. Conventional PCR was performed for the detection of virulence genes. Biofilm formation capacity was assessed by tissue culture plate assay (TCP). The antimicrobial susceptibility of the isolates was evaluated using the dilution method.ResultsIn total, 37 sequence types (ST) and 34 Staphylococcal protein A (spa) types (including a new type named spa-t20720) were identified based on multilocus sequence typing (MLST) and spa typing, respectively. CC8-ST239-t030-agrI-SCCmecIII (57.6%,34/59) and CC7-ST7-t091-agrI (16.1%, 5/31) represented the main clone of MRSA and MSSA isolates, respectively. Antibiotic susceptibility testing identified a significant difference in resistance rates between ST239 and non-ST239 isolates (p < 0.05). Twelve virulence genes were detected, of which the most common were icaA and icaD (both 100%), followed by icaB and icaC (both 96.7%), icaR (95.6%), lukED (81.1%), lukAB (62.2%), pvl (50%), hlgBC (26.7%), and eta (4.4%). Moreover, lukAB, hlgBC, agrI, and agrIII were significantly correlated with burn severity (p < 0.05). MRSA isolates were less likely, compared with MSSA isolates, to carry pvl, lukAB, and hlgBC (p < 0.05). A new spa type, t20720, was identified that contains pvl, lukED, lukAB, hlgBC, icaA, icaB, icaC, icaD, and icaR genes and has strong biofilm formation ability.ConclusionCC8-ST239-t030-agrI-SCCmecIII and CC7-ST-7-t091-agrI were the prevalent molecular signatures of MRSA and MSSA isolates from burn wound infections in Fujian, China, respectively. The newly identified spa-t20720 isolate, which carries a wide range of virulence genes and has strong biofilm formation ability, requires special clinical attention.

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“…The effects of proteins and EPS on staphylococci biofilms should be clarified. Gene expression linked with biofilm production in S. aureus has received minimal attention [9][10][11]. The present study aims to understand how proteins affect staphylococci biofilms.…”

Section: Introductionmentioning

confidence: 99%

Gene Expression Evaluation of Intracellular Adhesins and Regulatory Genes among Biofilm Producing MRSA Isolates

Abdrabaa

Aburesha

2023

Iraqi Journal of Science

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The gene expression of the most important structural genes ica A and D of biofilm, sarA, and sigB regulatory genes of some methicillin-resistant Staphylococcus aureus (MRSA) isolates were examined using the real-time polymerase chain reaction after 24 hours of growth. The results revealed that the isolates with strong biofilm production had the highest gene expression of the structural icaA and D genes. Whereas the isolates that showed moderate and weak biofilm production, recorded the lowest gene expression. The results of the regulatory genes sarA, and sigB fluctuated among all MRSA isolates. Isolate No. 64 recorded the highest gene expression, while isolate 50 recorded the lowest gene expression. The purpose of this study was to determine the gene expression of structural genes ica A and D, and regulator genes sigB and sarA in some MRSA isolates with different abilities of biofilm formation where the sarA and sigB play important role in positive regulation of biofilm formation.

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Biofilm formation and molecular analysis of intercellular adhesion gene cluster (icaABCD) among Staphylococcus aureus strains isolated from children with adenoiditis

Cited by 8 publications

References 24 publications

Detection of mecA Genes in Hospital-Acquired MRSA and SOSA Strains Associated with Biofilm Formation

Detection of mecA Genes in Hospital-Acquired MRSA and SOSA Strains Associated with Biofilm Formation

Molecular typing and characterization of Staphylococcus aureus isolates from burn wound infections in Fujian, China

Gene Expression Evaluation of Intracellular Adhesins and Regulatory Genes among Biofilm Producing MRSA Isolates

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