Biofilm formation and resistance

In vivocharacterization of electroactive biofilms inside porous electrodes with MR Imaging

“…For example, the cell count (not including EPS-matrix) or the total biomass in the electrode can be determined using qPCR assays or determination of the total nitrogen content. 61,71,72 The validation we applied by qPCR and correlation of current per biomass (see ESI† for details) cannot confirm quantitatively the biofilm density but show a similar trend of a higher biofilm density in the improved medium M*.…”

The protective role of caffeic acid on bovine mammary epithelial cells and the inhibition of growth and biofilm formation of Gram-negative bacteria isolated from clinical mastitis milk

“…It is notable that antimicrobial actions mainly occur when changing the permeability of cell membranes, enzyme activity, biofilm formation, or the structure of proteins and DNA, as well as blocking the adhesion or internalization of host cells. In the case of bovine mastitis infections caused by pathogenic bacteria, biofilm penetrating the teat and sticking to the internal surface of mammary tissue is suggested to help develop MDR in E. coli strains ( 42 , 43 ). Therefore, taking into consideration the blocking of biofilm formation to potentiate the elimination of troublesome pathogens is one way of finding alternatives to antibacterial agents.…”

Combining hyperspectral imaging and electrochemical sensing for detection of Pseudomonas aeruginosa through pyocyanin production

“…3,9 An alternative method to culture is to use molecular techniques for pathogen identification, but these do not discriminate between biofilm-constituting organisms and extracellular DNA or planktonic bacteria contained in a sample. 2,3,16 Recent fluorescence-based techniques and next-generation sequencing have not yet found their place in the clinic due to cost and expertise requirements, 3 and no consensus has been reached over standardisation or quantitative strategies. 10,17 Furthermore, low replicability both within and between laboratories does not facilitate comparisons and development of universal models, detection, treatments or point-of-care solutions.…”