Biofilm Formation and Virulence Determinants of Klebsiella oxytoca Clinical Isolates from Patients with Colorectal Cancer

Abbas, Aalaa Fahim; Al-Saadi, Aamal Ghazi Mahdi; Alkhudhairy, Miaad K.

doi:10.1007/s12029-019-00317-7

Cited by 8 publications

(8 citation statements)

References 31 publications

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“…Given this expectation, as well as the efficiency with which the V. cholerae machinery directs RNA-guided transposition in E. coli, we set out to evaluate INTEGRATE activity in other Gram-negative bacteria. We selected Klebsiella oxytoca, a clinically relevant pathogen implicated in drug-resistant infections 50 and emerging model organism for biorefinery 51 , and Pseudomonas putida, an important bacterial platform for biotechnological and industrial applications 52,53 (Fig. 5a).…”

Section: Broad Host-range Activity Of Rna-guided Integrasesmentioning

confidence: 99%

CRISPR RNA-guided integrases for high-efficiency, multiplexed bacterial genome engineering

et al. 2020

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Section: Broad Host-range Activity Of Rna-guided Integrasesmentioning

confidence: 99%

CRISPR RNA-guided integrases for high-efficiency, multiplexed bacterial genome engineering

et al. 2020

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“…Antibiotic-associated hemorrhagic colitis (AAHC) is a disease caused by the expansion of colitogenic strains of Klebsiella oxytoca in some patients after antibiotics such as penicillin treatment [158]. Recently biofilms of K. oxytoca were identified in patients with CRC [159].…”

Section: Klebsiella Oxytoca Enterotoxinsmentioning

confidence: 99%

The Role of DNA Damage Response in Dysbiosis-Induced Colorectal Cancer

Rivas-Domínguez

Pastor

Martínez-López

et al. 2021

Cells

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The high incidence of colorectal cancer (CRC) in developed countries indicates a predominant role of the environment as a causative factor. Natural gut microbiota provides multiple benefits to humans. Dysbiosis is characterized by an unbalanced microbiota and causes intestinal damage and inflammation. The latter is a common denominator in many cancers including CRC. Indeed, in an inflammation scenario, cellular growth is promoted and immune cells release Reactive Oxygen Species (ROS) and Reactive Nitrogen Species (RNS), which cause DNA damage. Apart from that, many metabolites from the diet are converted into DNA damaging agents by microbiota and some bacteria deliver DNA damaging toxins in dysbiosis conditions as well. The interactions between diet, microbiota, inflammation, and CRC are not the result of a straightforward relationship, but rather a network of multifactorial interactions that deserve deep consideration, as their consequences are not yet fully elucidated. In this paper, we will review the influence of dysbiosis in the induction of DNA damage and CRC.

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“…The evolution of carbapenemase-producing (CP) strains has limited last-line treatment resorts. These strains may develop the resistance through various mechanisms such as production of carbapenemase enzymes [ 2 , 3 ]. Some of these enzymes include imipenemases (IMPs), OXA-type beta-lactamase, and New Delhi metallo-β-lactamase 1 (NDM-1) [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

“…Biofilm formation is another strategy of K. oxytoca for cell attachment and colonization. In K. oxytoca , fimA, mrkA, pilQ and matB play substantial role in bacterial attachment and colonization [ 3 , 8 ]. FimA, MrkA and MatB are the major protein subunits of type 1 ( fimBEAICDFGH operon), type 3 ( mrkABCDF operon) and Mat fimbriae, respectively which recognized in uropathogenic Escherichia coli and can mediate adhesion and biofilm formation [ 9 – 11 ].…”

Section: Introductionmentioning

confidence: 99%

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Synergistic effects of silybin and curcumin on virulence and carbapenemase genes expression in multidrug resistant Klebsiella oxytoca

et al. 2022

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Objective Silybin and curcumin have potential antimicrobial effects. This study aimed to evaluate the synergistic antimicrobial effects of silybin and curcumin on virulence and carbapenemase genes expression among multidrug-resistant (MDR) Klebsiella oxytoca. Results A total of 70 MDR K. oxytoca (carrying blaIMP and blaOXA-48-like genes) were included. The antibiotic susceptibility and biofilm production of isolates were determined. The silybin and curcumin at concentrations 10–500 mg/mL alone and in combination were exposed to bacterial isolates in Mueller Hinton broth medium for 24 h. The expression of blaIMP, blaOXA-48-like, mrkA, pilQ, matB and fimA genes was evaluated using quantitative real-time polymerase chain reaction (qRT-PCR). The mean minimum inhibitory concentration (MIC) of curcumin and silybin were 250 mg/mL and 500 mg/mL, respectively. The anti-virulent effect of 100 mg/mL of silybin and curcumin was shown by significant reduction in the expression of fimA (2.1-fold, P < 0.0001) and mrkA (2.1 fold, P < 0.0001) genes. Moreover, these compounds significantly decreased the expression of blaIMP1 (3.2-fold, P < 0.0001) gene. Notably, there was no significant effect on pilQ, matB and blaOXA-48-like genes. The results showed that silybin and curcumin can be candidate as natural way for control the MDR virulent strains of K. oxytoca.

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Biofilm Formation and Virulence Determinants of Klebsiella oxytoca Clinical Isolates from Patients with Colorectal Cancer

Cited by 8 publications

References 31 publications

CRISPR RNA-guided integrases for high-efficiency, multiplexed bacterial genome engineering

CRISPR RNA-guided integrases for high-efficiency, multiplexed bacterial genome engineering

The Role of DNA Damage Response in Dysbiosis-Induced Colorectal Cancer

Synergistic effects of silybin and curcumin on virulence and carbapenemase genes expression in multidrug resistant Klebsiella oxytoca

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