Biofilm formation by Staphylococcus aureus strains and their control by selected phytochemicals

Abstract

Biofilm formation by 30 Staphylococcus aureus dairy isolates and their control by phytochemicals were investigated. The majority of strains were biofilm positive by phenotypic analysis. The nuc and icaA genes were present in 30 and 27 strains, respectively. In addition, 13 strains were positive for all nuc, clfA, fnbA and icaA genes. The antimicrobial and antibiofilm activities of citral, cinnamaldehyde, eugenol, farnesol, limonene and terpinen‐4‐ol were also evaluated for seven strains. It was shown that the use of farnesol, cinnamaldehyde or terpinen‐4‐ol at a concentration of 2 mg/mL could be at least 80% effective on S. aureus strains and their biofilms.

Terpinen-4-ol as an Antibacterial and Antibiofilm Agent against Staphylococcus aureus

“…The activity of terpinen-4-ol has been described in the literature, and it is believed that the substance is primarily responsible for the antibacterial activity of the essential oil of M. alternifolia , as there is a similarity in the antibacterial efficacy of the essential oil and the isolated compound [ 9 , 10 , 11 , 12 ]. In addition to the antibacterial activity of terpinen-4-ol, good antibiofilm activity of the substance was observed against some biofilm-forming species, which highlights its potential for application in clinical practice [ 10 , 13 , 14 ]. However, there are still few studies on the activity of terpinen-4-ol against biofilms formed by S. aureus .…”

Impact of ultrasound treatment on viability of Staphylococcus aureus and the human milk antioxidant activity

“…The S.aureus strain can contaminate dairy products during processing and infect humans, so this bacterium plays a significant role in public health (Unlu et al, 2018). In addition, diseases caused by the presence of pathogens in dairy products due to inadequate hygiene practices are the greatest concerns of consumers, researchers and food regulatory agencies (Zavareh & Ardestani, 2020).…”

Enzyme-based approaches to control microbial biofilms in dairy processing environments: A review

“…The ability of S. aureus to form biofilms is also an important virulence factor since this mechanism guarantees its survival in a new environment (Watters et al, 2016). Materials such as stainless steel, glass and polypropylene have proven to be the sources of contamination by S. aureus after undergoing adhesion and subsequently forming biofilms (Lee et al, 2014;Unlu et al, 2018). L. monocytogenes is another pathogenic bacterium whose biofilms represent a significant problem in dairy processing areas, mainly because of the contamination of food handling surfaces, equipment and pipes (Kadam et al, 2013).…”