Biofilm formation by Porphyromonas gingivalis and Streptococcus gordonii

Abstract

Confocal scanning laser microscopy (CSLM) was used to visualize and quantify biofilm formation by the oral bacteria Streptococcus gordonii and Porphyromonas gingivalis. A saliva‐coated glass coverslip under continuous bacterial challenge and conditions of low shear force was used to investigate attachment to the salivary pellicle and also the effect of cell‐cell interactions on the extent of colonization and biofilm development. S. gordonii bound to the salivary pellicle and outcompeted P. gingivalis for attachment sites. Both P. gingivalis and S. gordonii failed to establish substantial biofilm formation independently. However, biofilm formation did occur subsequent to initial adherence of P. gingivalis to S. gordonii cells deposited on the salivary pellicle. The commensal species S. gordonii may, therefore, provide an attachment substrate for colonization and biofilm accretion by the potential pathogen, P. gingivalis.

Platelet aggregation promoted by biofilms of oral bacteria and the effect of mouth rinses in vitro

“…P. gingivalis adherence to oral streptococci is multimodal and involves at least two distinct sets of adhesins and receptors [27]. In vitro, P. gingivalis adheres avidly to sessile streptococci, and once attached, it rapidly forms a biofilm comprising towering microcolonies separated by fluid-filled channels [28]. S. sanguinis has the ability to generate hydrogen peroxide, which is a key factor in the antagonistic interaction between S. sanguinis and P. gingivalis [29].…”

Novel Model for Multispecies Biofilms That Uses Rigid Gas-Permeable Lenses

“…Genetically distinct oral bacteria have been shown to adhere to tooth enamel and to one another by several specific adhesinreceptor interactions. Research from various groups demonstrated that members of the Streptococcus genus are the early colonizers that anchor the biofilms to saliva-coated substratum in vitro and reflect in vivo biofilm initiation (11,34,35), and, in particular, S. gordonii demonstrates this property (8,17). Intraspecies coaggregation of streptococci has been shown by extensive coaggregation analysis (23); however, in our study, unique sequential deposition of taxonomically related coaggregation partners such as S. gordonii, S. oralis, and S. sanguinis was also observed.…”

Capacidade de dissolução do hipoclorito de sódio e da clorexidina sobre biofilme oral formado 'in situ'

“…Autoclave utilizada para esterilização dos blocos (C) A periodontite apical é a principal patologia associada com o fracasso do tratamento endodôntico, se encontrando nela uma vasta variedade de espécies bacterianas (SKUCAITE et al, 2010;ZIJNGE et al, 2010). No entanto, a maioria dos trabalhos analisa o poder antimicrobiano dos irrigantes endodônticos contra espécies bacterianas isoladas (JACINTO et al, 2003;EICK, SELTMANN, PFISTER, 2004;DUNAVANT et al, 2006;CLEGG et al, 2006;DUGGAN, SEDGLEY, 2007;GIARDINO et al, 2007;OZOK et al, 2007;SEDLACEK, WALKER, 2007;CAMARA et al, 2009;ARIAS-MOLIZ et al, 2010;CHAVEZ DE PAZ, BERGENHOLTZ, SVENSATER, 2010), não permitindo as interações bacterianas características dos biofilmes multi-espécie que os fazem mais resistentes (COOK, COSTERTON, LAMONT, 1998;STANDAR et al, 2010;ZIJNGE et al, 2010).…”

“…Os estudos in vitro são muito importantes para determinar as interações bacterianas que ocorrem em um biofilme (KOLENBRANDER et al, 1993;COOK;LAMONT, 1998;PALMER et al, 2001;DIAZ et al, 2006;STANDAR et al, 2010), mas não são capazes de simular de uma forma exata as condições de crescimento bacteriano na boca, como por exemplo, a diversidade de nutrientes, mudanças de humidade e pH, temperatura, saliva; etc.…”