Biofilm formation in plant–microbe associations

Ramey, Bronwyn E.; Koutsoudis, Maria D.; Bodman, Susanne B. von; Fuqua, Clay

doi:10.1016/j.mib.2004.10.014

Cited by 364 publications

(236 citation statements)

References 69 publications

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“…At this stage, filamentous structures were observed in the microscopic analysis, apparently promoting bacteria-to-bacteria and bacteria-to-plant surface cross-linking. Similar observations have been described in other endophytic interactions, resembling bacterial polysaccharide-like structures that may point to crucial stages of ligand-receptor apparatuses with significant potential to modulate the plant-bacteria interactions (Bacilio-Jiménez et al 2001, James et al 1994, Ramey et al 2004, Roncato-Maccari et al 2003. However, the nature and origin of these fibrils present on inoculated rice are unknown yet.…”

Section: Discussionsupporting

confidence: 56%

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Endophytic colonization of rice (Oryza sativa L.) by the diazotrophic bacterium Burkholderia kururiensis and its ability to enhance plant growth

Mattos

Pádua

Romeiro

et al. 2008

An. Acad. Bras. Ciênc.

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Burkholderia kururiensis is a diazotrophic bacterium originally isolated from a polluted aquifer environment and presents a high level of similarity with the rice endophyte "B. brasilensis" species. This work assessed the ability of B. kururiensis to endophytically colonize rice plantlets by monitoring different tissues of root-inoculated plants for the presence of bacterial growth in different media, electron microscopy and by 16S rDNA analysis. Observations of roots, stems and leaves of inoculated rice plantlets by electron microscopy revealed B. kururiensis colonization predominantly on root hair zones, demonstrating endophytic colonization primarily through the endodermis, followed by spreading into xylem vessels, a possible pathway leading to aerial parts. Although indifferent for the bacterial growth itself, addition of a nitrogen source was a limiting factor for endophytic colonization. As endophytic colonization was directly associated to an enhanced plant development, production of phytohormone auxin/indole-3-acetic acid by B. kururiensis was assayed with transgenic rice plantlets containing an auxin-responsive reporter (DR5-GUS). Our findings suggest the ability of auxin production by plant-associated B. kururiensis which may have a stimulatory effect on plant development, as evidenced by activation of DR5-GUS. We hereby demonstrate, for the first time, the ability of B. kururiensis to endophytically colonize rice, promoting both plant growth and rice grain yield.

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Section: Discussionsupporting

confidence: 56%

“…age) and nitrogen fertilization (FuentesRamírez et al 1993). Remarkably, sensitivity of many diazotrophic bacteria to nitrogen fertilizers could affect their endophytic ability (Fuentes-Ramírez et al 1993, Munõz and Caballero-Mellado 2003, Muthukumarasamy et al 2002, Ramey et al 2004, Tan et al 2003.…”

Section: Discussionmentioning

confidence: 99%

Endophytic colonization of rice (Oryza sativa L.) by the diazotrophic bacterium Burkholderia kururiensis and its ability to enhance plant growth

Mattos

Pádua

Romeiro

et al. 2008

An. Acad. Bras. Ciênc.

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“…T he recent research emphasis in bacterial cell-cell communication (1)(2)(3)(4)(5) and microbial biofilms (6)(7)(8)(9) has reinforced the concept that bacteria are social organisms that exist as multicellular entities in nature. Multicellular bacterial development and morphogenesis is well described in species of Myxobacterium, Caulobacter, and Bacillus (10)(11)(12), and similar, though perhaps less dramatic, coordinate behaviors exist in many other bacteria.…”

mentioning

confidence: 99%

“…Both bacterial groups frequently produce a second class of furanone-based QS signals that are implicated in interspeciesspecific communication (18). Many bacteria depend on QS regulated gene systems to establish symbiotic or pathogenic interactions with eukaryotic hosts or to colonize inanimate natural habitats (8,(19)(20)(21)(22)(23)(24)(25). It is also recognized as a key mechanism for governing various aspects of biofilm development, including adhesion, motility, maturation, and dispersion (7,9,26,27).…”

mentioning

confidence: 99%

Quorum-sensing regulation governs bacterial adhesion, biofilm development, and host colonization in Pantoea stewartii subspecies stewartii

Koutsoudis¹,

Tsaltas

Minogue

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

221

166

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The phytopathogenic bacterium Pantoea stewartii subsp. stewartii synthesizes stewartan exo͞capsular polysaccharide (EPS) in a cell density-dependent manner governed by the EsaI͞EsaR quorumsensing (QS) system. This study analyzes biofilm development and host colonization of the WT and QS regulatory mutant strains of P. stewartii. First, we show that the cell density-dependent synthesis of stewartan EPS, governed by the EsaI͞EsaR QS system, is required for proper bacterial adhesion and development of spatially defined, 3D biofilms. Second, a nonvirulent mutant lacking the esaI gene adheres strongly to surfaces and develops densely packed, less structurally defined biofilms in vitro. This strain appears to be arrested in a low cell density developmental mode. Exposure of this strain to exogenous N-acyl-homoserine lactone counteracts this adhesion phenotype. Third, QS mutants lacking the EsaR repressor attach poorly to surfaces and form amorphous biofilms heavily enmeshed in excess EPS. Fourth, the WT strain disseminates efficiently within the xylem, primarily in a basipetal direction. In contrast, the two QS mutant strains remain largely localized at the site of infection. Fifth, and most significantly, epifluorescence microscopic imaging of infected leaf tissue and excised xylem vessels reveals that the bacteria colonize the xylem with unexpected specificity, particularly toward the annular rings and spiral secondary wall thickenings of protoxylem, as opposed to indiscriminate growth to fill the xylem lumen. These observations are significant to bacterial plant pathogenesis in general and may reveal targets for disease control.maize ͉ xylem ͉ pathogenesis ͉ dissemination

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“…Similar to our results, Fujiwara et al (2013) reported that rhizosphere microbes in organic hydroponics successfully suppressed FOL. Furthermore, the colonization of microbes on the plant root surface, also known as biofilm (Bogino et al 2013;Ramey et al 2014), has protective effects against pathogens (Ramamoorthy et al 2001;Haggag 2010;Saharan & Nehra 2011).…”

Section: Research Articlementioning

confidence: 99%

Organic hydroponics induces systemic resistance against the air-borne pathogen,Botrytis cinerea(gray mould)

Chinta

Eguchi

Widiastuti

et al. 2015

Journal of Plant Interactions

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Here, we propose that organic hydroponics trigger induced systemic resistance (ISR) in lettuce against air-borne Botrytis cinerea, which causes gray mold. We compared effects of organic and chemical hydroponics, assessed presence of ISR elicitors in the hydroponic nutrient solution, and investigated molecular mechanism of ISR. Organic hydroponics significantly reduced gray mold lesions in lettuce (cultivated hydroponically) and cucumber (cultivated in soil and foliar sprayed with nutrient solution). The 1-aminocyclopropane-1-carboxylic acid synthase gene in lettuce and lipoxygenase and ethylene receptor-related gene in cucumber showed heightened expression, suggesting that the jasmonic acid/ethylene (JA/ET)-signaling pathway was involved in ISR for both crops. Low salicylic acid β-glucoside levels confirmed role of the ISR signaling pathway. ISR in both lettuce and cucumbers indicated that elicitors in organic hydroponics were nonhost-specific and that the JA/ET pathway was activated without microbe-root interaction. Thus, organic hydroponics can be an effective method for both soil-borne and air-borne disease control.

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Biofilm formation in plant–microbe associations

Cited by 364 publications

References 69 publications

Endophytic colonization of rice (Oryza sativa L.) by the diazotrophic bacterium Burkholderia kururiensis and its ability to enhance plant growth

Endophytic colonization of rice (Oryza sativa L.) by the diazotrophic bacterium Burkholderia kururiensis and its ability to enhance plant growth

Quorum-sensing regulation governs bacterial adhesion, biofilm development, and host colonization in Pantoea stewartii subspecies stewartii

Organic hydroponics induces systemic resistance against the air-borne pathogen,Botrytis cinerea(gray mould)

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