Biofilm Formation Under In Vitro Conditions

Trappetti, Claudia; Oggioni, Marco R.

doi:10.1016/b978-0-12-410530-0.00013-2

Cited by 3 publications

(1 citation statement)

References 45 publications

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“…In the continuous flow operation mode, fresh medium is continuously added to the reactor, and the effluent is discarded at the same rate at which the fresh media is added. Trappetti et al [105] suggested continuous flow bioreactors as the most suitable for studying the mechanisms and growth of mature biofilms; specifically, they can help with developing an understanding of the spatial arrangements of the structure and the EPS of a biofilm. Continuous flow processes allow users to collect samples at different time points, thereby extending the productivity of the study, and due to the addition of fresh nutrients, the overall issue related to heat generation in the bioreactor can be eliminated, with the enabling of better temperature control.…”

Section: Continuous Flow Processmentioning

confidence: 99%

Multispecies Bacterial Biofilms and Their Evaluation Using Bioreactors

Prabhukhot,

Eggleton,

Patel

2023

Foods

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Pathogenic biofilm formation within food processing industries raises a serious public health and safety concern, and places burdens on the economy. Biofilm formation on equipment surfaces is a rather complex phenomenon, wherein multiple steps are involved in bacterial biofilm formation. In this review we discuss the stages of biofilm formation, the existing literature on the impact of surface properties and shear stress on biofilms, types of bioreactors, and antimicrobial coatings. The review underscores the significance of prioritizing biofilm prevention strategies as a first line of defense, followed by control measures. Utilizing specific biofilm eradication strategies as opposed to a uniform approach is crucial because biofilms exhibit different behavioral outcomes even amongst the same species when the environmental conditions change. This review is geared towards biofilm researchers and food safety experts, and seeks to derive insights into the scope of biofilm formation, prevention, and control. The use of suitable bioreactors is paramount to understanding the mechanisms of biofilm formation. The findings provide useful information to researchers involved in bioreactor selection for biofilm investigation, and food processors in surfaces with novel antimicrobial coatings, which provide minimal bacterial attachment.

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Section: Continuous Flow Processmentioning

confidence: 99%

Multispecies Bacterial Biofilms and Their Evaluation Using Bioreactors

Prabhukhot,

Eggleton,

Patel

2023

Foods

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Biological puzzles solved by using Streptococcus pneumoniae : a historical review of the pneumococcal studies that have impacted medicine and shaped molecular bacteriology

Hiller,

Orihuela

2024

J Bacteriol

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The major human pathogen Streptococcus pneumoniae has been the subject of intensive clinical and basic scientific study for over 140 years. In multiple instances, these efforts have resulted in major breakthroughs in our understanding of basic biological principles as well as fundamental tenets of bacterial pathogenesis, immunology, vaccinology, and genetics. Discoveries made with S. pneumoniae have led to multiple major public health victories that have saved the lives of millions. Studies on S. pneumoniae continue today, where this bacterium is being used to dissect the impact of the host on disease processes, as a powerful cell biology model, and to better understand the consequence of human actions on commensal bacteria at the population level. Herein we review the major findings, i.e., puzzle pieces, made with S. pneumoniae and how, over the years, they have come together to shape our understanding of this bacterium’s biology and the practice of medicine and modern molecular biology.

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Comparative Study of Garlic Extract Effect on the Expression of Genes Involved in Quorum Sensing in Pseudomonas aeruginosa and the Antibiotic Tobramycin

Zadeh

Bidhendi

Ashrafi

2021

Iran J Med Microbiol

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Background: The expression of most genes involved in pathogenesis in Pseudomonas aeruginosa is controlled and regulated by a gene system called quorum sensing (QS). The purpose of this study was to investigate the quorum quenching of garlic extract in preventing the expression of genes involved in QS system of P. aeruginosa.Methods: In the present cross-sectional study, 12 P. aeruginosa strains were collected from burn wounds, cultured on special media and then confirmed by differential tests. The PCR test was performed to detect the lasI and lasR genes. The expression level of lasI gene was assessed in the presence of garlic extract and tobramycin antibiotic by real-time PCR, and the active ingredient of garlic extract was analyzed by HPLC.Results: Examination of 12 strains cultured on special and differential media showed that all 12 strains were P. aeruginosa. The PCR results indicated that all strains had 100% lasI and lasR genes. The real-time PCR results also revealed that the garlic extract was able to reduce the expression level of lasI gene in P. aeruginosa. It was found that tobramycin has a greater ability to reduce the expression level of this gene compared with garlic extract. Conclusion:This study showed that the garlic extract could reduce the expression of lasI gene in P. aeruginosa isolates, and that the active ingredients of this extract could be used to treat infections as an alternative to antibiotic therapy.

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Biofilm Formation Under In Vitro Conditions

Cited by 3 publications

References 45 publications

Multispecies Bacterial Biofilms and Their Evaluation Using Bioreactors

Multispecies Bacterial Biofilms and Their Evaluation Using Bioreactors

Biological puzzles solved by using Streptococcus pneumoniae : a historical review of the pneumococcal studies that have impacted medicine and shaped molecular bacteriology

Comparative Study of Garlic Extract Effect on the Expression of Genes Involved in Quorum Sensing in Pseudomonas aeruginosa and the Antibiotic Tobramycin

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