Biofilm Forming Multi Drug Resistant Staphylococcus spp. among Patients with Conjunctivitis

Murugan, Kasi; Usha, MG; Malathi, P.; Al-Sohaibani, A Saleh; Chandrasekaran, M.

doi:10.33073/pjm-2010-036

Cited by 30 publications

(22 citation statements)

References 48 publications

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“…The biofilm formation ability among CNS isolates appears to be unrelated to the clinical stage of the conjunctiva; these results in cats contrast with the results obtained in human medicine (Hou et al 2012 ; Murugan et al 2010 ). However, it should be noted that the feline conjunctivitis is usually caused by primary infection with viruses (FHV-1), Mycoplasma spp., or Chlamydophila spp.…”

Section: Discussioncontrasting

confidence: 58%

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Identification of bap and icaA genes involved in biofilm formation in coagulase negative staphylococci isolated from feline conjunctiva

et al. 2014

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Bap and icaA genes are commonly known to be involved in the biofilm formation. The prevalence of bap and icaA genes and biofilm formation was determined in conjunctival isolates of coagulase negative staphylococci (CNS) collected from cats. The study was conducted on 90 archival CNS isolates collected from feline conjunctiva obtained from clinically healthy cats and cats with ocular problems. Biofilm formation was examined using the microtiter plate (MTP) method. The prevalence of icaA and bap genes was determined using polymerase chain reaction (PCR). Genetic profiles of the bap-positive isolates were examined using the modified random amplified polymorphic DNA (RAPD) method. Of the 90 CNS isolates investigated, 58.9 % (53/90) were confirmed to form biofilms on a polystyrene plate after 24 h, and the intensity of the biofilm production varied strongly between positive strains. Among the biofilm-producing isolates, 24.5 % (13/53) carried the icaA gene and 3.8 % (2/53) carried the bap gene. Among the isolates that did not produce biofilms, the icaA gene and bap gene were detected in 8.1 % (3/37) and 2.7 % (1/37) of isolates, respectively. This is the first report demonstrating that CNS isolated from feline conjunctiva can potentially be a bap gene reservoir. Preliminary comparison of the genetic profiles of three bap-positive isolates collected from cats showed that each of the isolates has a different genetic background with a high similarity with the human strain of S. epidermidis.

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Section: Discussioncontrasting

confidence: 58%

“…Murugan et al have isolated multi-drug resistant Staphylococcus spp. from clinical cases of conjunctivitis and have confirmed that approximately 90 % of them can produce biofilms (Murugan et al 2010 ). Biofilms are also implicated in the pathogenesis of acute conjunctivitis related to punctal plugs (Yokoi et al 2000 ).…”

Section: Discussionmentioning

confidence: 92%

Identification of bap and icaA genes involved in biofilm formation in coagulase negative staphylococci isolated from feline conjunctiva

et al. 2014

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“…The tested bacteria must always be able to adhere on the surface of electrode at the bottom in the measuring well [ 7 , 38 ]. The ability to destroy the biofilm formation is one of the virulence factors in bacteria with low sensitivity to ATBs [ 39 ].…”

Section: Resultsmentioning

confidence: 99%

Staphylococcus aureus and MRSA Growth and Biofilm Formation after Treatment with Antibiotics and SeNPs

Číhalová

Chudobová

Michálek

et al. 2015

IJMS

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Methicillin-resistant Staphylococcus aureus (MRSA) is a dangerous pathogen resistant to β-lactam antibiotics. Due to its resistance, it is difficult to manage the infections caused by this strain. We examined this issue in terms of observation of the growth properties and ability to form biofilms in sensitive S. aureus and MRSA after the application of antibiotics (ATBs)—ampicillin, oxacillin and penicillin—and complexes of selenium nanoparticles (SeNPs) with these ATBs. The results suggest the strong inhibition effect of SeNPs in complexes with conventional ATBs. Using the impedance method, a higher disruption of biofilms was observed after the application of ATB complexes with SeNPs compared to the group exposed to ATBs without SeNPs. The biofilm formation was intensely inhibited (up to 99% ± 7% for S. aureus and up to 94% ± 4% for MRSA) after application of SeNPs in comparison with bacteria without antibacterial compounds whereas ATBs without SeNPs inhibited S. aureus up to 79% ± 5% and MRSA up to 16% ± 2% only. The obtained results provide a basis for the use of SeNPs as a tool for the treatment of bacterial infections, which can be complicated because of increasing resistance of bacteria to conventional ATB drugs.

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“…The absence of icaA gene detected in icaD gene positive S. aureus isolates, the same authors explained with the possibility of mutation in the icaA gene. Using the sequencing analysis, Murugan et al [27] have found a high similarity, but some differences in the icaA genes sequences of the 24 S. aureus isolates from conjunctivitis patients. Szweda et al [28] pointed out that some mutations in ica genes are possible and this polymorphism may be the reason for the failed amplifi cation of these genes.…”

Section: Discussionmentioning

confidence: 99%

Detection of icaA and icaD genes of Staphylococcus aureus isolated in cases of bovine mastitis in the Republic of Serbia

et al. 2017

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Staphylococcus aureus (S. aureus) is the most common agent of contagious mastitis that causes serious health and economic problems. The ability to form biofilms is an important virulence factor of S. aureus for the establishment of persistent infections. This study is aimed to investigate the presence of icaA and icaD, two genes of importance for the biofilm formation in S. aureus bovine mastitis isolates. In order to isolate and identify S. aureus, 1555 milk samples were collected from 401 cows, located in different regions of the Republic of Serbia. Using the conventional microbiological methods 100 isolates were characterized as coagulase-positive staphylococci. After primary biochemical identification, the species confirmation of 44 S. aureus isolates was done using MALDI-TOF Mass Spectrometry and PCR technique, targeting the S. aureus-specific nuc gene. Among all investigated S. aureus isolates 25.0% harbored both icaA gene and icaD genes. The presence of icaD gene alone was confirmed in 40.9% of cases totaling icaD positive isolates to 65.9%. The remaining 34.1% of S. aureus isolates were negative for the presence of both genes. The results of the present study indicate the existence of potential biofilm-producer strains in different regions of the Republic of Serbia, both under intensive and semi-extensive cows breeding.

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Biofilm Forming Multi Drug Resistant Staphylococcus spp. among Patients with Conjunctivitis

Cited by 30 publications

References 48 publications

Identification of bap and icaA genes involved in biofilm formation in coagulase negative staphylococci isolated from feline conjunctiva

Identification of bap and icaA genes involved in biofilm formation in coagulase negative staphylococci isolated from feline conjunctiva

Staphylococcus aureus and MRSA Growth and Biofilm Formation after Treatment with Antibiotics and SeNPs

Detection of icaA and icaD genes of Staphylococcus aureus isolated in cases of bovine mastitis in the Republic of Serbia

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