Biofilm Inactivation and Prevention on Common Implant Material Surfaces by Nonthermal DBD Plasma Treatment

Ibis, Fatma; Oflaz, Hakan; Ercan, Utku Kürşat

doi:10.1615/plasmamed.2016015846

Cited by 25 publications

(25 citation statements)

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“…For XTT assay, 2 ml of 0.5‐mg/ml XTT was added to each ET fragment and incubated at 37°C for 4 hr in a stationary incubator. The 4‐hr incubation time in XTT assay was selected according to manufacturers' protocol, our previous work, [ 31 ] and a brief optimization experiment where 4‐hr incubation was found to be needed for complete color development of XTT. After incubation, 100 µl of reacted XTT was transferred to a 96‐well plate and absorbance was measured at 496 nm.…”

Section: Methodsmentioning

confidence: 99%

Inactivation of biofilms in endotracheal tube by cold atmospheric plasma treatment for control and prevention of ventilator‐associated pneumonia

Ibis

Ercan

2020

Plasma Processes & Polymers

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Ventilator-associated pneumonia (VAP) is a type of hospital-acquired infection with high mortality and morbidity rates that is seen in critically ill patients who are connected to mechanical ventilators in intensive care units. Conventional infection control measures may remain insufficient for control and prevention of VAP. In the present study, biofilms of most common pathogens, which are responsible for VAP, namely Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcus aureus, and Candida albicans, were grown in the lumen of endotracheal tubes (ETs) under low shear to mimic the biofilm formation in clinical settings using the drip flow reactor system. Antibiofilm efficacies of two different plasma treatment methods were tested on the biofilms that were grown in ETs. In the first method, deionized water (DIW), phosphate-buffered saline (PBS) solution, and N-acetylcysteine (NAC) solution were treated with air dielectric barrier discharge (DBD) plasma and then nebulized on the biofilms. In the second method, air plasma afterglow (APA), which was generated using a DBD jet electrode, was flown through the lumen of ETs. Effects of two different plasma treatment methods on biofilms were evaluated using colony-counting and XTT assays. The quantity of extracellular polymeric substance (EPS) was evaluated with the safranin assay. Scanning electron microscopy (SEM) was used to visualize the effect of various plasma treatment methods on biofilms. Cytotoxic effects of two different plasma treatment methods were tested on the human tracheal epithelial cell line. Our results suggest that the nebulized plasma-treated NAC solution had a superior antibiofilm effect as compared with DIW and PBS. Two different plasma treatment methods used effectively inactivated biofilms and significantly reduced the quantity of EPS without a significant damage to the human tracheal epithelial cell line. Furthermore, SEM images showed the inactivation of microbial cells mainly through membrane damage. K E Y W O R D S cold plasma, dielectric barrier discharges, endotracheal tube, plasma medicine, ventilatorassociated pneumonia Fatma İbiş and Utku K. Ercan contributed equally to this study.

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Section: Methodsmentioning

confidence: 99%

Inactivation of biofilms in endotracheal tube by cold atmospheric plasma treatment for control and prevention of ventilator‐associated pneumonia

Ibis

Ercan

2020

Plasma Processes & Polymers

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“…However, it is well known that CAP has also shown tremendous effect on eradication and prevention of bacterial biofilms 191. While CAP and NP combination is attributed as an attractive strategy inefficient inactivation of MDR pathogens, similar strategies have not been tested for the elimination of bacterial biofilms 192. The field is lack of findings on combinatory CAP and NP to fight against biofilm‐associated bacterial infections.…”

Section: Nanomaterial‐integrated Diagnosis Prevention and Therapy Omentioning

confidence: 99%

Evolving Technologies and Strategies for Combating Antibacterial Resistance in the Advent of the Postantibiotic Era

Karaman

Ercan

Bakay

et al. 2020

Adv Funct Materials

132

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The threats posed by the impending "postantibiotic era" have put forward urgent challenges to be overcome by providing new diagnostic and therapeutic regimes for improved diagnosis and treatment of bacterial infections. Antibiotic resistance and incurable bacterial infections are especially important in a society faced with rapid demographic changes. With very few new antibiotics in the drug development pipeline, not being able to match the pace of antimicrobial resistance evolution, developments within other fields such as materials sciences and medical technologies are required to realize innovative antibacterial approaches. This progress report presents recent advances in especially nanotechnology-based approaches and their concomitant use with complementary antibacterial treatments. Synergistically improved antibacterial activity can be reached by considering novel, promising approaches such as photodynamic and photothermal therapy as well as cold atmospheric pressure treatments as complementary strategies to fight against antibacterial resistance. Moreover, this report describes how these novel technologies can be further improved especially by integration of nanomaterials into the currently applied single modal strategies against bacterial infections.

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“…titanyum malzemelerin plazma ön muamelesi ile hidrofilisitenin artırılmasına bağlı olarak implant yüzeyinde biyofilm oluşumunun azaltılabileceğini göstermiş olup, böyle bir plazma ön muamelesi sonucunda titanyum implantların yerleştirilmesi ardından peri-implantitisin engellenebileceği veya azaltılabileceği ortaya atılmıştır. 94…”

Section: Atmosferik Basınçlı Soğuk Plazma Aracılı Yüzey Biyomodifikaunclassified

The fourth state of matter: Plasma and applications of atmospheric pressure cold plasmas ın dentistry

Küçük¹,

Ercan²,

Köseoğlu³

2018

Yeditepe J Dent

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Maddenin 4. hali olan plazma içerdiği aktif plazma bileşenleri; pozitif ve negatif iyonlar, elektronlar, fotonlar, ultraviyole, uyarılmış atomlar veya moleküller, radikaller, nötral atom veya moleküller sayesinde sanayide, endüstride, gıda mühendisliği ve tekstil gibi birçok alanda kullanılmaktadır. Tıp ve diş hekimliğinde canlı dokularda zarar oluşturmaksızın dezenfeksiyon sağlanması ve yara iyileşmesinin hızlandırılması hala güncel ve önemli bir konudur. Plazmanın bir çeşidi olan atmosferik basınçlı soğuk plazmalar ısıl etki oluşturmaksızın dezenfeksiyon, hücre-doku biyostimülasyonu, yüzey modifikasyon ve/ veya aktivasyon etkinliklerini gerçekleştirmektedir. Bu derleme yazısında plazma hakkında genel bilgiler verildikten sonra tıpta, diş hekimliğinde ve periodontolojide atmosferik basınçlı soğuk plazmaların kullanımı anlatılmıştır. Anahtar kelimeler: Plazma gazları, termal olmayan atmosferik basınçlı plazma, diş hekimliği, dezenfeksiyon, yara iyileşmesi SUMMARY The fourth state of matter, plasma, contains active components such as: positive and negative ions, electrons, photons, ultraviolet, stimulated atoms/molecules, radicals and neutral atoms/molecules. Plasmas are used in many different industries including food and textile engineering. In medicine and dentistry, disinfecting and ensuring rapid regeneration of living tissue without harm is an emerging isssue. One variety of plasma referred as atomospheric pressure cold plasma, accomplishes disinfection, cell-tissue biostimulation, surface modification and/or activation without producing thermal effects. In this review we aim to provide general information about plasma, and how it is essential in understanding the utilization of atmospheric pressure cold plasma in medicine, dentistry and periodontology.

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Biofilm Inactivation and Prevention on Common Implant Material Surfaces by Nonthermal DBD Plasma Treatment

Cited by 25 publications

References 0 publications

Inactivation of biofilms in endotracheal tube by cold atmospheric plasma treatment for control and prevention of ventilator‐associated pneumonia

Inactivation of biofilms in endotracheal tube by cold atmospheric plasma treatment for control and prevention of ventilator‐associated pneumonia

Evolving Technologies and Strategies for Combating Antibacterial Resistance in the Advent of the Postantibiotic Era

The fourth state of matter: Plasma and applications of atmospheric pressure cold plasmas ın dentistry

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