Biofilm inhibition in oral pathogens by nanodiamonds

Zhang, Tongtong; Kalimuthu, Shanthini; Rajasekar, Vidhyashree; Xu, Feng; Yiu, Yau Chuen; Hui, Tony K.C.; Neelakantan, Prasanna; Chu, Zhiqin

doi:10.1039/d1bm00608h

Cited by 7 publications

(5 citation statements)

References 76 publications

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“…For instance, DBM dysbiosis could be managed by antimicrobial photodynamic therapy, which can eliminate pathogens with no risk of the emergence of drug-resistant strains (56). DBM dysbiosis could also be countervailed with the use of nanoparticles that alters DBM composition by interfering in fundamental biofilm properties such as adhesion and quorum-sensing (57,58). These innovative approaches will be instrumental to evaluate whether early interventions to correct DBM dysbiosis can prevent aGVHD onset.…”

Section: Discussionmentioning

confidence: 99%

Dental Biofilm Microbiota Dysbiosis Is Associated With the Risk of Acute Graft-Versus-Host Disease After Allogeneic Hematopoietic Stem Cell Transplantation

et al. 2021

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Acute graft-versus-host disease (aGVHD) is one of the major causes of death after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Recently, aGVHD onset was linked to intestinal microbiota (IM) dysbiosis. However, other bacterial-rich gastrointestinal sites, such as the mouth, which hosts several distinctive microbiotas, may also impact the risk of GVHD. The dental biofilm microbiota (DBM) is highly diverse and, like the IM, interacts with host cells and modulates immune homeostasis. We characterized changes in the DBM of patients during allo-HSCT and evaluated whether the DBM could be associated with the risk of aGVHD. DBM dysbiosis during allo-HSCT was marked by a gradual loss of bacterial diversity and changes in DBM genera composition, with commensal genera reductions and potentially pathogenic bacteria overgrowths. High Streptococcus and high Corynebacterium relative abundance at preconditioning were associated with a higher risk of aGVHD (67% vs. 33%; HR = 2.89, P = 0.04 and 73% vs. 37%; HR = 2.74, P = 0.04, respectively), while high Veillonella relative abundance was associated with a lower risk of aGVHD (27% vs. 73%; HR = 0.24, P < 0.01). Enterococcus faecalis bloom during allo-HSCT was observed in 17% of allo-HSCT recipients and was associated with a higher risk of aGVHD (100% vs. 40%; HR = 4.07, P < 0.001) and severe aGVHD (60% vs. 12%; HR = 6.82, P = 0.01). To the best of our knowledge, this is the first study demonstrating that DBM dysbiosis is associated with the aGVHD risk after allo-HSCT.

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Section: Discussionmentioning

confidence: 99%

Dental Biofilm Microbiota Dysbiosis Is Associated With the Risk of Acute Graft-Versus-Host Disease After Allogeneic Hematopoietic Stem Cell Transplantation

et al. 2021

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“…Clinically, basic periodontal treatment, periodontal surgery, and drug treatment (mostly antibiotics) are often used to improve pathogenic bacteria removal as well as periodontitis and peri-implantitis treatment [ 7 ]. However, traditional antibiotics are restricted by the induction of antibiotic resistance and their low efficiency to kill bacteria in biofilms, which imposes certain limitations on clinical applications [ 8 , 9 ]. Identifying a biological agent that does not produce bacterial resistance and inhibits biofilm formation is important.…”

Section: Introductionmentioning

confidence: 99%

Physicochemical, Antibacterial Properties, and Compatibility of ZnO-NP/Chitosan/β-Glycerophosphate Composite Hydrogels

Huang

Han

Lin

et al. 2022

J. Microbiol. Biotechnol.

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In this study we aimed to develop novel ZnO-NP/chitosan/β-glycerophosphate (ZnO-NP/CS/β-GP) antibacterial hydrogels for biomedical applications. According to the mass fraction ratio of ZnO-NPs to chitosan, mixtures of 1, 3, and 5% ZnO-NPs/CS/β-GP were prepared. Using the test-tube inversion method, scanning electron microscopy and Fourier-transform infrared spectroscopy, the influence of ZnO-NPs on gelation time, chemical composition, and cross-sectional microstructures were evaluated. Adding ZnO-NPs significantly improved the hydrogel's antibacterial activity as determined by bacteriostatic zone and colony counting. The hydrogel's bacteriostatic mechanism was investigated using live/dead fluorescent staining and scanning electron microscopy. In addition, crystal violet staining and MTT assay demonstrated that ZnO-NPs/CS/β-GP exhibited good antibacterial activity in inhibiting the formation of biofilms and eradicating existing biofilms. CCK-8 and live/dead cell staining methods revealed that the cell viability of gingival fibroblasts (L929) cocultured with hydrogel in each group was above 90% after 24, 48, and 72 h. These results suggest that ZnO-NPs improve the temperature sensitivity and bacteriostatic performance of chitosan/β-glycerophosphate (CS/β-GP), which could be injected into the periodontal pocket in solution form and quickly transformed into hydrogel adhesion on the gingiva, allowing for a straightforward and convenient procedure. In conclusion, ZnO-NP/CS/β-GP thermosensitive hydrogels could be expected to be utilized as adjuvant drugs for clinical prevention and treatment of peri-implant inflammation.

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“…Of particular interest is the antifungal activity of CNPs. Candida species cause various diseases of the oral cavity: caries, including in young children, and infections of the oropharyngeal mucosa [ 29 ]. C. glabrata is thought to have both innate and acquired resistance to antifungal drugs.…”

Section: Resultsmentioning

confidence: 99%

“…The antibacterial activity of nanodiamonds can be due to the negatively charged acid anhydride groups present on the surface [ 27 , 28 ]. Nanodiamonds are effective not only against planktonic cells, but also against biofilms [ 29 ]. For example, mannose-modified NDs have in particular shown a high potential in countering E. coli -biofilm formation [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

Fullerenes on a Nanodiamond Platform Demonstrate Antibacterial Activity with Low Cytotoxicity

et al. 2023

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Carbon nanoparticles with antimicrobial properties, such as fullerenes, can be distinguished among the promising means of combating pathogens characterized by resistance to commercial antibiotics. However, they have a number of limitations for their use in medicine. In particular, the insolubility of carbon nanoparticles in water leads to a low biocompatibility and especially strong aggregation when transferred to liquid media. To overcome the negative factors and enhance the action of fullerenes in an extended range of applications, for example, in antimicrobial photodynamic therapy, we created new water-soluble complexes containing, in addition to C60 fullerene, purified detonation nanodiamonds (AC960) and/or polyvinylpyrrolidone (PVP). The in vitro antibacterial activity and toxicity to human cells of the three-component complex C60+AC960+PVP were analyzed in comparison with binary C60+PVP and C60+AC960. All complexes showed a low toxicity to cultured human skin fibroblasts and ECV lines, as well as significant antimicrobial activity, which depend on the type of microorganisms exposed, the chemical composition of the complex, its dosage and exposure time. Complex C60+PVP+AC960 at a concentration of 175 µg/mL showed the most stable and pronounced inhibitory microbicidal/microbiostatic effect.

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Biofilm inhibition in oral pathogens by nanodiamonds

Abstract: Nanodiamonds were demonstrated to have great effects on inhibiting biofilm formation as well as disrupting the preformed biofilm in several selected orally and systemically important organisms.

Cited by 7 publications

References 76 publications

Dental Biofilm Microbiota Dysbiosis Is Associated With the Risk of Acute Graft-Versus-Host Disease After Allogeneic Hematopoietic Stem Cell Transplantation

Dental Biofilm Microbiota Dysbiosis Is Associated With the Risk of Acute Graft-Versus-Host Disease After Allogeneic Hematopoietic Stem Cell Transplantation

Physicochemical, Antibacterial Properties, and Compatibility of ZnO-NP/Chitosan/β-Glycerophosphate Composite Hydrogels

Fullerenes on a Nanodiamond Platform Demonstrate Antibacterial Activity with Low Cytotoxicity

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