Biofilm inspired fabrication of functional bacterial cellulose through ex-situ and in-situ approaches

Gilmour, Katie; Aljannat, Mahab; Markwell, Christopher; James, Paul; Jordan, Scott; Jiang, Yunhong; Torun, Hamdi; Dade‐Robertson, Martyn; Zhang, Meng

doi:10.1016/j.carbpol.2022.120482

Cited by 11 publications

(7 citation statements)

References 48 publications

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“…Experimental procedures were carried out to express recombinant BslA in E. coli and produce bacterial cellulose non‐woven fabric (Gilmour et al, 2023 ), which was then used to prepare various natural and synthetic textile samples. The textiles were treated with CFE solutions containing BslA with a double cellulose binding module (dCBM) and tested for fire retardancy according to a modified British standard protocol (Figure 1 ).…”

Section: Resultsmentioning

confidence: 99%

“…The cellulose producing bacterium Komagataeibacter xylinus DSM 2325 was used to produce non‐woven fabric in Hestrin–Schramm (HS) medium. A starter culture was prepared as previously described (Gilmour et al, 2023 ) and pellicles were grown in 300 mL of HS media in Microboxes (160 mm × 160 mm, SacO 2 , Belgium). After 14 days growth, pellicles were harvested and washed in dH 2 O for 3 h, then overnight.…”

Section: Methodsmentioning

confidence: 99%

“…Here we investigate the potential of a bacterial hydrophobic protein, BslA which stands for biofilm surface layer protein A (Kobayashi & Iwano, 2012 ; Morris et al, 2017 ), to infer fire retardancy on synthetic and natural fibres. The limitation that has been identified with using proteins on textiles for the purposes of functionalization has been their lack of adhesion to the textile particularly following washing, however, by using an engineered biology approach to produce BslA with an adhesive domain, cellulose binding module (CBM), the bind efficiency and binding durability of the protein to cellulosic textiles post‐washing can be increased (Florea et al, 2016 ; Gilbert et al, 2021 ; Gilmour et al, 2023 ; Griffo et al, 2019 ). We demonstrated that a wide range of fabrics treated with our engineered proteins have better fire retardancy.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Innovating fire safety with recombinant hydrophobic proteins for textile fire retardancy

Gilmour,

Arnadottir,

James

et al. 2023

Microbial Biotechnology

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Fire retardancy for textiles is important to prevent the rapid spread of fire and minimize damage to property and harm to human life. To infer fire‐resistance on textile materials such as cotton or nylon, chemical coatings are often used. These chemicals are usually toxic, and economically and environmentally unsustainable, however, some naturally produced protein‐based fire retardants could be an alternative. A biofilm protein from Bacillus subtilis (BslA) was identified and recombinantly expressed in Escherichia coli with a double cellulose binding domain. It was then applied to a range of natural and synthetic fabric materials. A flame retardancy test found that use of BslA reduced fire damage by up to 51% and would pass fire retardancy testing according to British standards. It is therefore a viable and sustainable alternative to current industrial fire‐retardant coatings.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Innovating fire safety with recombinant hydrophobic proteins for textile fire retardancy

Gilmour,

Arnadottir,

James

et al. 2023

Microbial Biotechnology

Self Cite

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“…Materials & Methods: 3. Expression of recombinant BslA in E. coli Recombinant BslA and green fluorescent protein (GFP) with a double CBM (dCBM) were produced in Escherichia coli as described previously [7]. In brief, the pET 28a containing corresponding sequences transformed into E. coli BL21 (DE3).…”

Section: Cfe Cell Free Extractmentioning

confidence: 99%

“…The cellulose producing bacterium Komagataeibacter xylinus DSM 2325 was used to produce non-woven fabric in Hestrin-Schramm (HS) medium. A starter culture was prepared as previously described [7] and pellicles were grown in 300 mL of HS media in Microboxes (160 mm x 160 mm, SacO2, Belgium). After 14 days growth, pellicles were harvested and washed in dH 2 O for 3 hours, then overnight.…”

Section: Bacterial Cellulose Non-woven Fabric Productionmentioning

confidence: 99%

Revolutionizing Textile Safety: Harnessing the Power of Recombinant Hydrophobic Proteins for Fire Retardancy

Gilmour

Arnardottir

James

et al. 2023

Preprint

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Fire retardancy for textiles is important to prevent the rapid spread of fire and minimize damage to property and harm to human life. To infer fire-resistance on textile materials such as cotton or nylon, chemical coatings are often used. These chemicals are usually toxic and economically and environmentally unsustainable, however, some naturally produced protein-based fire retardants could be an alternative. A biofilm protein from Bacillus subtilis (BslA) was identified and recombinantly expressed in Escherichia coli with a double cellulose binding domain. It was then applied to a range of natural and synthetic fabric materials. A flame retardancy test found that use of BslA reduced fire damage by up to 51% and would pass fire retardancy testing according to British standards. It is therefore a viable and sustainable alternative to current industrial fire-retardant coatings.

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Composites of 2D Materials and Bacterial Cellulose for Sustainable Energy Storage and Environmental Remediation

Gusain,

Kumar,

Seyedin

et al. 2024

Advanced Sustainable Systems

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The ever‐increasing demand for sustainable energy sources and environment protection is the focus of significant scientific scrutiny worldwide. Advanced composite materials have been developed to help meet the energy demand and environmental sustainability. Researchers are drawn to the study of two‐dimensional (2D) materials/bacterial cellulose (BC) composites for energy and environmental applications due to the intriguing electrical, mechanical, electrochemical, and catalytic properties of 2D materials combined with the sustainability and biocompatibility of BC. In this review, the key strategies are explained to develop 2D materials/BC composites and highlight unique properties of such fascinating systems. The recent progress on the application of advanced 2D materials/BC composites in energy storage (supercapacitors and batteries) and environmental remediation (water treatment, antimicrobial activity, and environmental gas sensing) are explained in detail highlighting future outlooks and challenges in the field. This review is intended to serve as a valuable resource for researchers currently engaged in the study of 2D materials and/or BC for different applications and is expected to shape the upcoming research and industrial applications of emerging 2D materials/BC composites.

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Biofilm inspired fabrication of functional bacterial cellulose through ex-situ and in-situ approaches

Cited by 11 publications

References 48 publications

Innovating fire safety with recombinant hydrophobic proteins for textile fire retardancy

Innovating fire safety with recombinant hydrophobic proteins for textile fire retardancy

Revolutionizing Textile Safety: Harnessing the Power of Recombinant Hydrophobic Proteins for Fire Retardancy

Composites of 2D Materials and Bacterial Cellulose for Sustainable Energy Storage and Environmental Remediation

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