Biofilm: New insights in the biological control of fruits with Bacillus amyloliquefaciens B4

Nie, Lin-Jie; Ye, Wan-Qiong; Xie, Wan-Yue; Zhou, Wenwen

doi:10.1016/j.micres.2022.127196

Cited by 9 publications

(2 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These beneficial bacteria can serve as an important foundation for constructing SynComs to control root rot in C. chinensis. Many Bacillus species have been reported to act as biocontrol agents for plant diseases (Ribeiro et al, 2021;Nie et al, 2022;Baard et al, 2023;Hernández-Huerta et al, 2023;Kadiri et al, 2023). According to previous reports, lipopeptide antimicrobial compounds play a key role in Bacillus to inhibit of the growth and development of plant pathogens (Amaning Danquah et al, 2022;Kim et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Bacillus velezensis LT1: a potential biocontrol agent for southern blight on Coptis chinensis

Tang,

Wang,

Huang

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

IntroductionSouthern blight, caused by Sclerotium rolfsii, poses a serious threat to the cultivation of Coptis chinensis, a plant with significant medicinal value. The overreliance on fungicides for controlling this pathogen has led to environmental concerns and resistance issues. There is an urgent need for alternative, sustainable disease management strategies.MethodsIn this study, Bacillus velezensis LT1 was isolated from the rhizosphere soil of diseased C. chinensis plants. Its biocontrol efficacy against S. rolfsii LC1 was evaluated through a confrontation assay. The antimicrobial lipopeptides in the fermentation liquid of B. velezensis LT1 were identified using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS). The effects of B. velezensis LT1 on the mycelial morphology of S. rolfsii LC1 were examined using scanning electron microscopy (SEM) and transmission electron microscopy (TEM).ResultsThe confrontation assay indicated that B. velezensis LT1 significantly inhibited the growth of S. rolfsii LC1, with an inhibition efficiency of 78.41%. MALDI-TOF-MS analysis detected the presence of bacillomycin, surfactin, iturin, and fengycin in the fermentation liquid, all known for their antifungal properties. SEM and TEM observations revealed that the mycelial and cellular structures of S. rolfsii LC1 were markedly distorted when exposed to B. velezensis LT1.DiscussionThe findings demonstrate that B. velezensis LT1 has considerable potential as a biocontrol agent against S. rolfsii LC1. The identified lipopeptides likely contribute to the antifungal activity, and the morphological damage to S. rolfsii LC1 suggests a mechanism of action. This study underscores the importance of exploring microbial biocontrol agents as a sustainable alternative to chemical fungicides in the management of plant diseases. Further research into the genetic and functional aspects of B. velezensis LT1 could provide deeper insights into its biocontrol mechanisms and facilitate its application in agriculture.

show abstract

Section: Discussionmentioning

confidence: 99%

Bacillus velezensis LT1: a potential biocontrol agent for southern blight on Coptis chinensis

Tang,

Wang,

Huang

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Currently, various antagonistic Bacillus species have been isolated and screened from soil, plants, and other environments, including B. amyloliquefaciens (Zheng et al, 2018;Wu et al, 2019;Jia et al, 2023;Markovićet al, 2023), B. velezensis (Reyes-Estebanez et al, 2020Chen et al, 2022;Yan et al, 2022), B. subtilis (Bais et al, 2004;Ding et al, 2017;Qiao et al, 2023), among others. Previous investigations highlight the inhibitory and anti-infection effects of B. amyloliquefaciens against pathogenic fungi causing diseases in rice, tomato, potato, brinjal and fruit (Jiao et al, 2020;Nie et al, 2022;Markovićet al, 2023;Pradhan et al, 2023;Zhang et al, 2023). B. amyloliquefaciens has demonstrated potent capabilities in crop disease control, but at present, it is only reported that B. amyloliquefaciens can promote the root growth of replanted Malus hupehensis Rehd.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Bacillus amyloliquefaciens BA-4 and its biocontrol potential against Fusarium-related apple replant disease

Li,

He,

Guo

et al. 2024

Front. Plant Sci.

View full text Add to dashboard Cite

Apple replant disease (ARD), caused by Fusarium pathogens, is a formidable threat to the renewal of apple varieties in China, necessitating the development of effective and sustainable control strategies. In this study, the bacterial strain BA-4 was isolated from the rhizosphere soil of healthy apple trees in a replanted orchard, demonstrating a broad-spectrum antifungal activity against five crucial apple fungal pathogens. Based on its morphology, physiological and biochemical traits, utilization of carbon sources, and Gram stain, strain BA-4 was tentatively identified as Bacillus amyloliquefaciens. Phylogenetic analysis using 16S rDNA and gyrB genes conclusively identified BA-4 as B. amyloliquefaciens. In-depth investigations into B. amyloliquefaciens BA-4 revealed that the strain possesses the capacity to could secrete cell wall degrading enzymes (protease and cellulase), produce molecules analogous to indole-3-acetic acid (IAA) and siderophores, and solubilize phosphorus and potassium. The diverse attributes observed in B. amyloliquefaciens BA-4 underscore its potential as a versatile microorganism with multifaceted benefits for both plant well-being and soil fertility. The extracellular metabolites produced by BA-4 displayed a robust inhibitory effect on Fusarium hyphal growth and spore germination, inducing irregular swelling, atrophy, and abnormal branching of fungal hyphae. In greenhouse experiments, BA-4 markedly reduced the disease index of Fusarium-related ARD, exhibiting protective and therapeutic efficiencies exceeding 80% and 50%, respectively. Moreover, BA-4 demonstrated plant-promoting abilities on both bean and Malus robusta Rehd. (MR) seedlings, leading to increased plant height and primary root length. Field experiments further validated the biocontrol effectiveness of BA-4, demonstrating its ability to mitigate ARD symptoms in MR seedlings with a notable 33.34% reduction in mortality rate and improved biomass. Additionally, BA-4 demonstrates robust and stable colonization capabilities in apple rhizosphere soil, particularly within the 10-20 cm soil layer, which indicates that it has long-term effectiveness potential in field conditions. Overall, B. amyloliquefaciens BA-4 emerges as a promising biocontrol agent with broad-spectrum antagonistic capabilities, positive effects on plant growth, and strong colonization abilities for the sustainable management of ARD in apple cultivation.

show abstract

Kale (Brassica oleracea L. var. acephala) rhizosphere bacteria suppress Pythium aphanidermatum-induced damping-off of cabbage, produce biofilm and antimicrobial volatile compounds

Essa Al-Rubkhi,

Mohammed Al-Sadi,

Janke

et al. 2024

Australasian Plant Pathol.

View full text Add to dashboard Cite

Biofilm: New insights in the biological control of fruits with Bacillus amyloliquefaciens B4

Cited by 9 publications

References 65 publications

Bacillus velezensis LT1: a potential biocontrol agent for southern blight on Coptis chinensis

Bacillus velezensis LT1: a potential biocontrol agent for southern blight on Coptis chinensis

Characterization of Bacillus amyloliquefaciens BA-4 and its biocontrol potential against Fusarium-related apple replant disease

Kale (Brassica oleracea L. var. acephala) rhizosphere bacteria suppress Pythium aphanidermatum-induced damping-off of cabbage, produce biofilm and antimicrobial volatile compounds

Contact Info

Product

Resources

About