Biofilm producing indigenous bacteria isolated from municipal sludge and their nutrient removal ability in moving bed biofilm reactor from the wastewater

Abstract

In the present study, improved moving bed biofilm reactor (MBBR) was applied to enhance the nutrient removal ability of the municipal wastewater. A total of 18 indigenous bacterial isolates were screened from the sewage sludge sample and nitrate reductase, nitrite reductase and hydroxylamine oxidase was analyzed. The strains Pseudomonas aeruginosa NU1 and Acinetobacter calcoaceticus K12 produced 0.87 ± 0.05 U/mg and 0.52 ± 0.12 U/mg hydroxylamine oxidase, 1.023 ± 0.062 U/mg and 1.29 ± 0.07 U/mg nitrite reductase, and 0.789 ± 0.031 U/mg and 1.07 ± 0.13 U/mg nitrate reductase. Nitrogen and phosphate removal improved by the addition of nutrient sources and achieved > 80% removal rate. pH and temperature of the medium also affected nutrient removal and improved removal was achieved at optimum level (p < 0.05). MBBR was designed with R1 (aerobic), R2 and R3 (anoxic) reactors. MBBR reactors removed acceptable level phosphorus removal properties up to 7.2 ± 3.8%, 42.4 ± 4.6%, and 84.2 ± 13.1% in the R1, R2, R3 and R4 reactors, respectively. Denitrification rate showed linear relationship at increasing concentrations nitrogen content in the reactor and denitrification rate was 1.43 g NO 2 -N /m 2 /day at 1.5 g NO 2 -N /m 2 /day. Dehydrogenase activity was assayed in all reactors and maximum amount was detected in the aerobic biofilm reactor. Based on the present findings, MBBRs and the selected bacterial strains are useful for the degradation domestic wastewater with minimum working area.

Growth Efficiency of Chlorella sorokiniana in Synthetic Media and Unsterilized Domestic Wastewater

“…Urban infrastructure creates conditions for the development of certain microbial communities in wastewater, which is rich in human waste. Representatives of the genera Pseudomonas , Acinetobacter , and Aeromonas are found in urban wastewaters and are capable of metabolizing various carbon and nitrogen compounds [ 60 , 61 ]. For example, Acinetobacter sp., isolated from municipal activated sludge, was able to actively remove inorganic nitrogen compounds by simultaneously performing nitrification and denitrification [ 62 ], similar to Pseudomonas spp., which is also actively involved in the nitrogen cycle [ 63 ].…”

Review of hospital effluents: special emphasis on characterization, impact, and treatment of pollutants and antibiotic resistance

“…Biological treatment uses a variety of microorganisms to biodegrade pollutants and eliminate EC (Nguyen et al, 2021). There are some techniques dedicated to the removal of BOD 5 , COD, and TSS, others for the elimination of bacteria, PhAC, and virus inactivation (Sá et al, 2021;Alam et al, 2021;Panneerselvam & Priya, 2021;Pourakbar et al, 2022) the performance of biological treatments that are widely used technologies for wastewater treatment in general and frequently implemented for HWW treatment such as ASP, MBR, constructed wetlands (CW), and moving bed bioreactors (MBBR) (Mir-Tutusaus et al, 2021;Alarjani, 2021;Sossalla et al, 2021;Leiviskä & Risteelä, 2022). ASP treatment is the most commonly applied process for wastewater treatment nowadays (Jari et al, 2022).…”

Structures and biological activity of three 2-(pyridin-2-yl)-1H-benzimidazole derivatives

“…Growing concern about antibiotic-resistant microorganisms is driving the search for new drugs for use against diseasecausing bacteria or yeasts (CDC, 2019). Hospital patients are at risk of infection from upper respiratory tract bacterial strains, such as Staphylococcus aureus, which can cause serious complications, including sepsis (Alarjani et al, 2021;Kholaseh et al, 2023). Candida yeasts can cause problems in transplant patients (Macias-Paz et al, 2023).…”