Biofilm Production and Antibiofilm Activity of Echinocandins and Liposomal Amphotericin B in Echinocandin-Resistant Yeast Species

Marcos-Zambrano, Laura Judith; Gómez-Perosanz, Marta; Escribano, Pilar; Zaragoza, Óscar; Bouza, Emilio; Guinea, Jesús

doi:10.1128/aac.03065-15

Cited by 18 publications

(5 citation statements)

References 29 publications

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“…Additionally, the measured concentrations were higher than ones needed for reduction in 50% of metabolic biofilm activity and most of the measured concentrations were higher than needed for reduction in 80% of metabolic biofilm activity for Candida spp. (0.29 and 7.89 mg/L respectively) [20]. The concentrations were also higher than reported after release from PMMA [9–11].…”

Section: Discussionmentioning

confidence: 84%

Joint fluid concentrations of amphotericin B after local application with calcium sulphate—report of 2 cases

Pozzi

Schläppi

Livio

et al. 2023

APMIS

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Fungal periprosthetic joint infections (PJI) are difficult to treat, due to important biofilm formation and limited local penetration of systemically administered antifungals. Calcium sulphate (CaSO 4 ) might be a promising carrier to increase local concentration of antifungals. We hypothesized that local amphotericin B release from CaSO 4 is high enough to significantly contribute to treatment of fungal PJI. We report joint fluid and serum concentrations of amphotericin B after local application with CaSO 4 as an implanted resorbable carrier material as adjunct to standard surgical and systemic antifungal treatment in two cases of PJI with Candida spp. Maximal joint fluid amphotericin B concentration was 14.01 mg/L 5 days after the second local administration of liposomal amphotericin in Case One and 25.77 mg/L 14 days after the second local administration in Case Two. Concentrations higher than minimal inhibitory concentrations (MIC) could be measured for 21 days and 17 days after local administration in Case One and Two, respectively. In Case Two, serum concentration of amphotericin B was <0.01 mg/L 3 days after local administration of 450 mg liposomal amphotericin B. No local or systemic adverse reaction was observed. Fungal PJI was successfully eradicated in both cases with a follow-up of 12 months in Case One and 20 months in Case Two. Application of amphotericin B-loaded CaSO 4 was associated with joint fluid concentrations higher than minimal inhibitory concentrations for Candida spp. for approximately 3 weeks, with the advantage that the carrier material dissolves spontaneously and does not require secondary removal. Relapse of fungal infections did not occur in these two patients.

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Section: Discussionmentioning

confidence: 84%

Joint fluid concentrations of amphotericin B after local application with calcium sulphate—report of 2 cases

Pozzi

Schläppi

Livio

et al. 2023

APMIS

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“…have been studied since the mid-1990s. In vitro experiments [ 3 , 18 , 22 , 23 , 24 , 25 , 26 , 27 , 28 ] are predominant compared to in vivo experiments [ 29 , 30 ] and confirmed the heterogeneity of these biofilms composed of dense layers of yeast blastopores, hyphal, pseudohyphae, and ECM [ 31 , 32 ]. Several genes are involved in the adhesion, ECM production, quorum sensing, and morphogenesis of biofilms, particularly in C. albicans [ 15 , 33 , 34 ].…”

Section: Yeasts and Filamentous Fungi Biofilmsmentioning

confidence: 85%

Fungal Biofilms and Polymicrobial Diseases

Costa-Orlandi

Sardi

Pitangui

et al. 2017

JoF

173

114

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Biofilm formation is an important virulence factor for pathogenic fungi. Both yeasts and filamentous fungi can adhere to biotic and abiotic surfaces, developing into highly organized communities that are resistant to antimicrobials and environmental conditions. In recent years, new genera of fungi have been correlated with biofilm formation. However, Candida biofilms remain the most widely studied from the morphological and molecular perspectives. Biofilms formed by yeast and filamentous fungi present differences, and studies of polymicrobial communities have become increasingly important. A key feature of resistance is the extracellular matrix, which covers and protects biofilm cells from the surrounding environment. Furthermore, to achieve cell–cell communication, microorganisms secrete quorum-sensing molecules that control their biological activities and behaviors and play a role in fungal resistance and pathogenicity. Several in vitro techniques have been developed to study fungal biofilms, from colorimetric methods to omics approaches that aim to identify new therapeutic strategies by developing new compounds to combat these microbial communities as well as new diagnostic tools to identify these complex formations in vivo. In this review, recent advances related to pathogenic fungal biofilms are addressed.

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“…Interestingly, the development of echinocandins occurred at the very same time that different groups were starting their pioneering work on Candida (mostly C. albicans ) biofilms. Early work in these academic laboratories in the late 1990s and early 2000s demonstrated the potent antifungal activity of physiological concentrations of echinocandins against C. albicans biofilms, with subsequent studies extended to other Candida spp [ 34 , 37 , 38 ].…”

Section: The Pastmentioning

confidence: 99%