Biofilm production and antibiotic susceptibility of <i>Staphylococcus epidermidis</i> strains from Hidradenitis Suppurativa lesions

Ardon, Christine B.; Prens, Errol P.; Fuursted, Kurt; Ejaz, Rahat; Shailes, J.; Jenssen, Håvard; Jemec, Gregor B. E.

doi:10.1111/jdv.15183

Cited by 29 publications

(33 citation statements)

References 43 publications

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“…Regarding the number of isolates in Tehran, Similar results were observed in the previously published studies. The frequency of S. epidermidis in other studies in Belgium 2013 (33), Africa 2018 (20), and Netherland 2019 (27) were assessed respectively [5][6][7]. Biofilm formation capability could enhance the durability of the mentioned bacterium against disinfection principles in hospitals in the current study [16].…”

Section: Discussionmentioning

confidence: 71%

“…The recent findings in Iran were shown S. epidermidis isolated strains from various clinical specimens more ubiquitous prevalence in other Iranian cities (73.9%) than Tehran (56.65%) [4]. Other worldwide conducted researches also have shown high isolation rates of the mentioned bacterium in clinical specimens, especially in medical devices samples [5][6][7]. Biofilms are notoriously resistant to immune system responses and antimicrobial agents.…”

Section: Introductionmentioning

confidence: 98%

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Genotypic and phenotypic analysis of biofilm formation Staphylococcus epidermidis isolates from clinical specimens

et al. 2020

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Objectives: Staphylococcus epidermidis is the primary causative agent of infections associated with indwelling biomaterials. Antibiotic susceptibility patterns, Biofilm formation capability, and screening of responsible genes in biofilm formation procedure in clinical isolates (icaA, icaB, icaC, icaD, sdrG, and atlE) were assigned as the main objectives in this study. The clinical samples were analyzed via standard biochemical assays for identifying different bacteria which were confirmed using the multiplex colony PCR method. Subsequently, biofilm-formation capability, antibiotic susceptibility testing, and the frequency of genes responsible for biofilm formation in the confirmed strains were checked. Results: Out of 183 clinical specimens 54 S. epidermidis isolates were detected by targeting a housekeeping gene (sesc) taking advantage of the PCR procedure. All of the strains were Biofilm forming producers. The in vitro biofilm formation assays determined that 45 (83.33%), 5 (9.26%), 4 (7.41%) were strong, moderate, and weak biofilm former strains respectively. Among the isolated strains, the specific frequencies of the biofilm-forming genes were specified to be (98%) for sdrG, (84%) for atlE, (80%) for icaC, and (70%) for icaD. Cefamandole and Amikacin are the most effective antibiotics in isolated strains. All strains were ascertained to be methicillin and amoxicillin/clavulanic acid resistant.

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Section: Discussionmentioning

confidence: 71%

Section: Introductionmentioning

confidence: 98%

Genotypic and phenotypic analysis of biofilm formation Staphylococcus epidermidis isolates from clinical specimens

et al. 2020

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“…The role of Staphylococcus spp. in the pathogenesis of hidradenitis suppurativa is still controversial, as it is not yet determined if inflammation follows colonization or the other way around [31,32]. Antibiotic treatment may help improve hidradenitis suppurativa lesions, but relapse of the disease is common after treatment interruption [29].…”

Section: Figure 1 Mechanisms Of Action For the Most Frequently Used mentioning

confidence: 99%

“…In the case of hidradenitis suppurativa, germs, including S. aureus and coagulase-negative staphylococci such as S epidermidis were found to be associated with the inflammatory aspects seen in this disease, but the relationship between them is not fully understood yet [31,32]. Bacterial colonization may lead to inflammation but also inflammatory lesions can become secondary infected [42].…”

Section: Figure 6 Distribution Of Antimicrobial Resistance Accordingmentioning

confidence: 99%

The Need to Develop New Antimicrobial Molecules, as Revealed by in vitro Assessment of Drug Resistance in Staphylococcal Skin Infections Treated with Autologous Bacterial Vaccine

Preda¹,

Serbanescu²,

Mihai³

et al. 2020

Rev. Chim.

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Staphylococcus spp. is a facultative pathogen, which can be found in the commensal microbiota of humans, most often in moist skinfolds and mucous membranes. This microorganism has the ability to cause various infections, in almost every organ of the body, with an increased frequency in the skin and soft tissues, being involved in pathologies like acne, folliculitis, furunculosis, hidradenitis suppurativa, cellulitis, abscesses, but also in secondary infections in diseases with an altered cutaneous barrier. The prolonged evolution of these diseases and severe outcome can be influenced by various factors, most importantly being the antimicrobial resistance. We have evaluated the antimicrobial susceptibility profiles, according to the Comite de l` Antibiogramme de la Societe Francaise de Microbiologie recommendations, for strains of Staphylococcus spp. isolated from acne or different types of skin and soft tissue infections in patients recommended to receive autologous bacterial vaccine. Most frequent identified species was Staphylococcus epidermidis, followed by Staphylococcus aureus. The antimicrobial resistance was higher for antibiotics usually used in the treatment of skin and soft tissue infections, with interesting differences of the resistance profile for the strains isolated from patients before receiving autologous bacterial vaccine compared with the ones from individuals already treated. Another important finding was represented by the differences in the resistance profile according to the age group of the patients. The results of this study underline the importance of antimicrobial resistance surveillance in finding new molecules and alternative therapies, the necessity of a personalized approach in medical acts and of a continuous connection between clinic and laboratory research.

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“…All strains were typed as described previously. 6 Rifampicin, tetracycline, clindamycin and doxycycline, commonly used to treat HS, were included in the experiments. Genetic relatedness of the S. lugdunensis strains was investigated with Pulsed-field gel electrophoresis (PFGE) 7 and analysed using BioNumerics software (version 5.0).…”

Section: Virulent Staphylococcus Lugdunensis With Limited Genetic DIVmentioning

confidence: 99%

Virulent Staphylococcus lugdunensis with limited genetic diversity in hidradenitis suppurativa lesions

Ardon

Prens

Tkadlec

et al. 2019

Acad Dermatol Venereol

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Biofilm production and antibiotic susceptibility of Staphylococcus epidermidis strains from Hidradenitis Suppurativa lesions

Cited by 29 publications

References 43 publications

Genotypic and phenotypic analysis of biofilm formation Staphylococcus epidermidis isolates from clinical specimens

Genotypic and phenotypic analysis of biofilm formation Staphylococcus epidermidis isolates from clinical specimens

The Need to Develop New Antimicrobial Molecules, as Revealed by in vitro Assessment of Drug Resistance in Staphylococcal Skin Infections Treated with Autologous Bacterial Vaccine

Virulent Staphylococcus lugdunensis with limited genetic diversity in hidradenitis suppurativa lesions

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