Biofilm removal by 6% sodium hypochlorite activated by different irrigation techniques

Ordinola‐Zapata, Ronald; Bramante, Clóvis Monteiro; Aprecio, Raydolfo M.; Handysides, Robert; Jaramillo, David E.

doi:10.1111/iej.12202

Cited by 115 publications

(101 citation statements)

References 34 publications

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“…Techniques which are commonly used, such as paper point sampling followed by CFU counting can determine general bacterial counting from the coronal third which can exclude information from the apical third, where disinfection is more indicative of irrigation efficiency and more difficult to access. Measurement of the apical third cleanliness has been attempted by direct-access methods, as well as split-model methods, both requiring structural modification of the dentin and apical complexities [17][18][19][20][21][22][23][24][25][26][27][28][29][30][31]. While the method used in this experiment contains similar shortcomings to the paper point/CFU method with regards to sampling location, it provides expeditious sample collection, reuse of samples in order for the same sample to serve as a control, and a well-known, reliable methodology for bacterial quantification.…”

Section: Discussionmentioning

confidence: 99%

“…Laser activation of intracanal irrigant creates large elliptical vapor bubbles that expand and subsequently implode, causing a cavitation and shock wave effect that promotes debridement and smear layer removal and the release of energy further down the canal [16]. Previous studies have shown PIPS to be effective in biofilm elimination [17], smear layer removal [16], and debris removal from complex nonseparated systems [18]. Apical size and taper size have been studied in the past by multiple studies, with varying conclusions reflecting the needs of obturation and irrigation techniques [19,20].…”

Section: Introductionmentioning

confidence: 99%

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In-Vitro Evaluation of the Efficacy of PIPS Irrigation System on Disinfection of Type 2 Canal Systems in Molars

Babu¹

2017

JDHODT

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Objective: This in vitro study was designed to compare the disinfection potential of an Er:YAG photon-initiated photoacousitc (PIPS) irrigation mechanism to standard needle irrigation (SNI) in eliminating biofilms from complex root canal systems. Method and materials:Twenty-four extracted mandibular molar teeth exhibiting a Vertucci Type II canal system were selected and underwent canal instrumentation to create either a minimal (20/.04) or fully-flared (25/.06) preparation. Teeth were inoculated with bacterial plaque and incubated for 3 weeks creating a mature biofilm. Both instrumentation groups were sub-divided according to mode of canal disinfection: sterile saline or sodium hypochlorite (NaOCl) with PIPS laser-activation or same irrigants delivered with SNI. Each irrigation protocol used similar quantities of NaOCl or saline over the same time periods. Viable intracanal bacteria were quantified by MTT bacterial viability assay. Sub-groups were analyzed for statistical significance by ANOVA, followed by Scheffe's F-test (P = .05).Results: All saline groups showed significantly more viable bacteria after irrigation than the corresponding NaOCl sub-groups. The #20/.04 sub-group that underwent PIPS and NaOCl irrigation had greater disinfection than the SNI counterpart, which was not significant (P > 0.05). The #25/.06 PIPS/NaOCl subgroup showed greater disinfection than SNI (P > 0.0008). All other groups showed no significant differences in disinfection of root canals. Conclusion:In this in vitro study PIPS laser-activated irrigation achieved higher disinfection in #25/.06 fully-flared preparations than SNI. Although not significantly different, greater disinfection was found in the #20/.04 minimal canal preparations that were treated with PIPS irrigation compared to SNI. Keywords: In-Vitro Evaluation of the Efficacy of PIPS Irrigation System on Disinfection of Type 2 Canal Systems in Molars 2/5Copyright: ©2017 Garcia-Godoy et al. [19,20]. The combination of varying tip size and taper continues to be studied, aimed at improving canal disinfection while conserving dentin. As apical preparation size increases, irrigant replacement was enhanced, providing space existed between the needle and the canal wall [21]. Increased canal taper also improved fluid interchange and reduced the risk of extrusion; minimal taper with large apical preparations also improved irrigant replacement [22]. Ex vivo investigation of the optimal tip size taper combination for laser activated irrigation have yet to be determined. The objective of this study was to examine the disinfection of intentionally contaminated Type II canals in the mesial root of mandibular molars by comparing needle flushing versus PIPS activation during endodontic irrigation of complex Vertucci Type II canal systems of two different sizes and taper. The null hypothesis was that no difference exists between bacterial disinfection present in #20/.04 taper and #25/.06 flared taper preparations using either PIPS or needle irrigation. Methods and MaterialsToot...

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In-Vitro Evaluation of the Efficacy of PIPS Irrigation System on Disinfection of Type 2 Canal Systems in Molars

Babu¹

2017

JDHODT

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“…Tijekom pasivnoga ultrazvučnog ispiranja (PUI) energija se prenosi s oscilirajućeg nastavka na sredstvo za ispiranje, uzrokujući stvaranje ultrazvučnih valova u tekućini (10). Pasivno ultrazvučno ispiranje pokazalo se učinkovitim u uklanjanju zaostatnog sloja i debrisa (11) te intrakanalnog biofilma (12). Sistem RinsEndo ® (Dürr Dental GmbH & Co., Bietigheim-Bissingen, Njemač-ka) automatski je sustav čije se djelovanje temelji na tehnologiji pritiska i povlačenja te na aktivaciji sredstva za ispiranje u korijenskom kanalu (1,5 Hz).…”

Section: Uvodunclassified

“…The PUI has been reported to be efficient in the removal of intracanal smear layer and debris (11), and to facilitate the disruption of endodontic biofilms (12). The RinsEndo ® (Dürr Dental GmbH & Co., Bietigheim-Bissingen, Germany) is an automated system which uses pressure-suction technology to deliver the irrigant solution in the root canal and activates it automatically (1.5 Hz).…”

Section: Izbor I Priprema Uzorakamentioning

confidence: 99%

Eradication of Intracanal Enterococcus Faecalis Biofilm by Passive Ultrasonic Irrigation and RinsEndo System

Toljan¹,

Bago²,

Jurič

et al. 2016

Acta Stomatol Croat

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“…Studies have shown that PUI with NaOCl could not only effectively remove the smear layer inside infected root canals but also significantly improve the cleaning of biofilm-infected dentine (George et al, 2008;De Moor et al, 2010;Ordinola-Zapata et al, 2014). For PUI, however, the apical third of a root canal should be enlarged to at least an International Organization for Standardization (ISO) size of 35-40 to allow needle placement to within 1-2 mm of the apical seat (Huang et al, 2008;Peeters & Gutknecht, 2014), which might limit its application.…”

Section: Introductionmentioning

confidence: 99%

Bactericidal effect of Er:YAG laser combined with sodium hypochlorite irrigation against Enterococcus faecalis deep inside dentinal tubules in experimentally infected root canals

Cheng

Chen

Qiu

et al. 2016

Journal of Medical Microbiology

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This study evaluated the bactericidal effect of Er:YAG laser radiation combined with sodium hypochlorite (NaOCl) irrigation in the treatment of Enterococcus faecalis deep inside dentinal tubules. The Er:YAG laser was activated, respectively, at 0.3, 0.5 and 1.0 W for either 20 or 30 s; 52.5 g l 21 NaOCl and normal saline were used for the control groups. Root canals before and after treatments were examined using scanning electron microscopy (SEM). Bacterial reductions both on the root canal walls and at 100, 200, 300, 400 and 500 mm inside the dentinal tubules were analysed using a one-way analysis of variance. SEM results showed that the Er:YAG laser combined with NaOCl disinfected the dentinal tubules from 200 to over 500 mm depth as irradiation power and time increased. This combination killed significantly more bacteria than both the negative control group at each level tested and the positive control group at 300, 400 and 500 mm inside the dentinal tubules. It reached 100 % in all experimental groups, both on the root canal walls and at 100 and 200 mm inside the dentinal tubules. However, at 300, 400 and 500 mm inside the dentinal tubules, only the groups treated with 0.5 and 1.0 W for 30s exhibited no bacterial growth. Of the two groups in which no bacteria were detected at all tested depths, Er:YAG laser irradiation at 0.5 W for 30 s combined with NaOCl irrigation was preferable because of the lower emission power and shorter irradiation time, and may serve as a new option for effective root canal disinfection.

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Biofilm removal by 6% sodium hypochlorite activated by different irrigation techniques

Abstract: Laser activation of 6% sodium hypochlorite significantly improved the cleaning of biofilm-infected dentine followed by passive ultrasonic irrigation.

Cited by 115 publications

References 34 publications

In-Vitro Evaluation of the Efficacy of PIPS Irrigation System on Disinfection of Type 2 Canal Systems in Molars

In-Vitro Evaluation of the Efficacy of PIPS Irrigation System on Disinfection of Type 2 Canal Systems in Molars

Eradication of Intracanal Enterococcus Faecalis Biofilm by Passive Ultrasonic Irrigation and RinsEndo System

Bactericidal effect of Er:YAG laser combined with sodium hypochlorite irrigation against Enterococcus faecalis deep inside dentinal tubules in experimentally infected root canals

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