Biofilm-Resistant Nanocoatings Based on ZnO Nanoparticles and Linalool

Abstract

Biofilms represent an increasing challenge in the medical practice worldwide, imposing a serious threat to public health. As bacterial strains have developed antibiotic resistance, researcher’s attention has been extensively focused on developing more efficient antimicrobial strategies. In this context, the present study reports the synthesis, physicochemical characterization, ex vivo biodistribution, and in vitro evaluation of the capacity of nanostructured surfaces based on zinc oxide (ZnO) and biologically active molecules to modulate clinically relevant microbial biofilms. ZnO nanoparticles (NPs) were synthesized through a co-precipitation method without thermal treatment. The matrix-assisted pulsed laser evaporation (MAPLE) was applied for preparing nanostructured coatings based on ZnO NPs surface modified with linalool that were further characterized by X-ray diffraction (XRD), thermogravimetric analysis with differential scanning calorimetry (TGA-DSC), scanning electron microscopy (SEM), transmission electron microscopy with selected area electron diffraction (TEM-SAED), Fourier-transform infrared spectroscopy (FT-IR), and infrared microscopy (IRM). Histological analyses carried out at 7 days and 14 days after the intraperitoneal administration of linalool modified ZnO NPs revealed the absence of the latter from the brain, kidney, liver, lung, myocardium, and pancreas. Through in vitro assays on prokaryotic cells, it was proven that ZnO coatings hinder microbial biofilm formation of both Gram-positive and Gram-negative bacteria strains.

A Review on Current Designation of Metallic Nanocomposite Hydrogel in Biomedical Applications

“…Various nanostructures, such as micelles, liposomes, dendrimers, metallic NPs, and nano-coatings, have been utilized in the development of nanotechnology, especially in the biomedicine field [ 12 , 13 , 14 , 15 , 16 ]. Among the various known NPs, metal or metal oxide NPs are the most promising ones due to their antibacterial properties in association with a high surface-to-volume ratio [ 17 ].…”

Nanocoating Is a New Way for Biofouling Prevention

“…Metal oxide nanorods and nanowires based photocatalytic coatings have attracted recent attention owing to its ease of controlled fabrication processes (Wei et al, 2005;Inderan et al, 2015;Jin et al, 2015a;Jin et al, 2015b;Wang et al, 2018). ZnO nanorod-based photocatalytic coatings have been found effective against micro-and macro-fouling on a glass substrate and fishing net substrates in the laboratory (Al-Fori et al, 2014;Spirescu et al, 2021) and field experiments, respectively (Sathe et al, 2017) (Figure 8). ZnO nanorods have increased stability, reduced loss of nanoparticles compared to ZnO nanoparticles and can be fabricated into various types of support materials (Al-Fori et al, 2014).…”

“…Nanostructured metal oxide, like ZnO and TiO 2 , is capable of absorbing visible and ultraviolet light and inhibiting microbial growth due to the photocatalytic process leading to redox reactions (Baruah et al, 2012;Danwittayakul et al, 2018;Spirescu et al, 2021). The generated reactive oxygen species (ROS) like peroxides, superoxides, and hydroxyl radicals that form during photocatalysis, prevent the growth of microorganisms on the surfaces (Sathe et al, 2017;Ganguly et al, 2018).…”

Optimization of Technological Parameters of the Process of Forming Therapeutic Biopolymer Nanofilled Films

“…The results of studies indicate the antibacterial activity of all three components with controlled release of the antibiotic. Spirescu et al [ 36 ] developed biofilms based on ZnO and linalool NPs. In vitro assays for prokaryotic cells have shown that biofilms prevent the formation of both gram-positive and gram-negative bacterial strains.…”