Biofilmes formados em matéria-prima em contato com leite: fatores de virulência envolvidos

Flach, Juliana; Karnopp, Carolina; Corção, Gertrudes

doi:10.22456/1679-9216.14953

Cited by 9 publications

(5 citation statements)

References 11 publications

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“…It was observed that although there was no growth of the S. aureus colonies after 24 hr at 25 °C, there was a growth of both species at this temperature after 48 hr. This demonstrates the superior ability of the Gram‐negative bacteria to adhere and adapt to surfaces in comparison with the Gram‐positive bacteria as previously reported by Flach et al (). This suggests that after the adhesion and growth of E. coli on the surface, S. aureus may have adhered to the EPS already formed by E. coli .…”

Section: Resultssupporting

confidence: 83%

Capacity ofEscherichia coliandStaphylococcus aureusto produce biofilm on stainless steel surfaces in the presence of food residues

Dutra

Férnandes

Perdoncini

et al. 2018

J Food Process Preserv

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This study analyzed biofilms composed of Escherichia coli and Staphylococcus aureus on a stainless steel surface, in the presence of food residues (milk and meat exudate), at 25 and 35 °C, with the objective of simulating the real conditions of industrial processing of these foods. The biofilm formed on the surface was determined by quantifying the colonies and scanning electron microscopy. In addition to milk and meat exudate, three different culture media were used, one specific for each bacterium and a common environment for the growth of both bacteria (Mueller Hinton agar, MH). Such conditions were favorable for the biofilm formation of both bacteria, with predominance of the Gram‐negative species in the multispecies biofilm. The food residues favored the formation of the biofilm in both temperatures, especially the meat exudate at 35 °C, when compared to the MH broth. Therefore, substrate and temperature directly influenced the microbial composition of the biofilm. Practical applications This study demonstrates the influence of industrial residue (substrate) on temperature in the composition of a biofilm. As it was evaluated two distinct bacteria, one Gram‐positive and another Gram‐negative, that can be present in several industrial food processing, it can be observed that the substrate and the temperature may favor the development of a species. This fact is extremely relevant so that a correct hygiene process can be applied and it is necessary to know the composition of the biofilm on a certain surface to combat it more effectively.

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Section: Resultssupporting

confidence: 83%

Capacity ofEscherichia coliandStaphylococcus aureusto produce biofilm on stainless steel surfaces in the presence of food residues

Dutra

Férnandes

Perdoncini

et al. 2018

J Food Process Preserv

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“…The ability of S. aureus isolates from milk to form biofilms has also been demonstrated. Fox, Zadoks, and Gaskins (2005) Factors related to physicochemical properties of the surface material, temperature, pH, and expression of virulence factors (Flach, Karnopp, & Corção, 2005) influence the attachment of macromolecules and, as a result, biofilm formation.…”

Section: Re Sults and Discussionmentioning

confidence: 99%

Phenotypic characterization of Staphylococcus aureus isolated from foods of animal origin and other related sources

Silva

Mello

Moraes

et al. 2020

J. Food Process. Preserv.

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The genus Staphylococcus spp. includes bacterial species that are opportunistic pathogens of humans and domestic animals as well. These microorganisms are commonly found on the skin and nasal cavities (Bannerman, 2003). These microorganisms are involved in several cases of food poisoning since some strains produce enterotoxins (Hasan, Hassawi, Al-Daghistani, & Hawari, 2014; Nitzsche, Zweifel, & Stephan, 2007). These enterotoxins are heat resistant. Therefore, once preformed are not eliminated during the thermal processing of food (Carmo et al., 2002) such as pasteurization of milk. Staphylococcal enterotoxins are detectable in food when Staphylococcus aureus counts are above 10 5 CFU/ml or

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“…Biofilms manifest unique developmental properties and phenotypic traits that favor protection from the external environment and provide better adhesion to surfaces. Thus, they reduce susceptibility to commercialized antimicrobials, becoming more resistant in environments with adverse conditions (Flach et al, 2005;Pasternak, 2009). The presence of biofilms causes surface deterioration and/or proliferation of pathogenic microorganisms, leading to damage in industry when present on equipment, and in health by increasing the mortality rate due to diseases caused by the resistance of bacteria to antimicrobials and disinfectants.…”

Section: Introductionmentioning

confidence: 99%

Investigation of phytochemical composition and bioactivity evaluation of extracts from Myrsine umbellata Mart.

Laskoski,

Batista,

Bandeira

et al. 2024

Braz. J. Biol.

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The objective of the study was to carry out phytochemical prospection through colorimetric tests to determine the groups of secondary metabolites and also to determine the total content of phenolic compounds (TPC) present in plant extracts methanol (ME), ethyl acetate (EAE), hexane (HE) and dichloromethane (DE) from the leaves of Myrsine umbellata, as well as to investigate the antimicrobial activity against twelve standard ATCC strains by the broth microdilution technique; the antioxidant potential by the DPPH method and the ABTS method and the antibiofilm potential on the biofilm biomass of standard bacteria by the crystal violet technique and tetrazolium salt reduction (MTT) assay. Phytochemical prospection detected the presence of saponins, steroids, alkaloids, anthocyanins, anthocyanidins, flavonoids, and tannins. The results of the quantitative phytochemical estimation revealed a higher content of total phenolics in DE (280.24 ± 0.037 µM GAE g ext. -1) followed by ME (159.01 ± 0.031 µM GAE g ext. -1). The ME showed the best biological activities when compared to the other extracts tested. We observed antimicrobial activity against Gram-positive Staphylococcus epidermidis strain (MIC 3.12 and MBC 6.25), antioxidant percentage of 92.58% against the DPPH radical and 420.31 µM Trolox g ext. -1 against the ABTS radical, finally showed antibiofilm action against Gram-positive strain Staphylococcus aureus, with eradication of the biomass in 92.58%. The results suggest that EM from M. umbellata represents an alternative source of plant bioactives for the development of natura products.

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Biofilmes formados em matéria-prima em contato com leite: fatores de virulência envolvidos

Cited by 9 publications

References 11 publications

Capacity ofEscherichia coliandStaphylococcus aureusto produce biofilm on stainless steel surfaces in the presence of food residues

Capacity ofEscherichia coliandStaphylococcus aureusto produce biofilm on stainless steel surfaces in the presence of food residues

Phenotypic characterization of Staphylococcus aureus isolated from foods of animal origin and other related sources

Investigation of phytochemical composition and bioactivity evaluation of extracts from Myrsine umbellata Mart.

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