Biofilms and Physical Deposits on Nasolacrimal Silastic Stents Following Dacryocystorhinostomy

Ali, Mohammad Javed; Baig, Fahd; Lakshman, Mekala; Naik, Milind N.

doi:10.1097/iop.0000000000000383

Cited by 15 publications

(12 citation statements)

References 10 publications

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“…Biofilms have been reported in both cases using mono® and bicanalicular stents. Further studies are needed in this grey area of canalicular stent selection [35][36][37][38]. In severe cases of primary canaliculitis with canalicular dilatation, the application of bicanalicular stents still came with favorable treatment outcomes in our study, while in mild or moderate cases of primary canaliculitis, monocanalicular stents may be theoretically more desirable.…”

Section: Discussionmentioning

confidence: 62%

Surgical procedure of canaliculoplasty in the treatment of primary canaliculitis associated with canalicular dilatation

Zhang

et al. 2020

BMC Ophthalmol

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Background: Primary canaliculitis is a chronic infection of the proximal lacrimal pathway. We aimed to evaluate surgical outcomes of a canaliculoplasty procedure for primary canaliculitis associated with canalicular dilatation. Methods: This study enrolled 42 primary canaliculitis patients with canalicular dilatation who underwent canaliculoplasty. All patients were treated with canaliculotomy, curettage of canalicular contents and canaliculoplasty with stent placement. Patients' demographics, clinical features, and follow-up outcomes were evaluated. Results: There were 12 males and 30 females with a mean age of 66.1 ± 13.9 years. The mean duration time from the first onset of signs/symptoms to diagnosis was 30.6 ± 39.5 months. Epiphora (90.5%) and mucopurulent discharge from punctum (85.7%) were the most common signs. Thirty-three out of 42 patients (78.6%) achieved complete remission with a mean follow-up time of 25.3 ± 12.9 months. There were 3 patients found to have canalicular stenosis due to obstruction after surgery. Conclusion: Canalicular dilatation is a severe condition of primary canaliculitis, probably due to a combined result of long standing disease and the presence of concretions. The surgical procedure of canaliculoplasty can be a highly effective treatment for primary canaliculitis associated with canalicular dilatation.

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Section: Discussionmentioning

confidence: 62%

Surgical procedure of canaliculoplasty in the treatment of primary canaliculitis associated with canalicular dilatation

Zhang

et al. 2020

BMC Ophthalmol

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“…It is not clear how long the stents should be left in place for optimal results. Studies on silastic stents used in DCR have shown that by 4 weeks, the stents become encased in biofilms, complex microbial communities, which could result in inflammation, infection and scarring 9, 10…”

Section: Discussionmentioning

confidence: 99%

Success rate of placement of a bicanalicular stent for partial nasolacrimal obstruction in adults under local, monitored anesthesia care and general anesthesia

Silbert¹

2017

Saudi Journal of Ophthalmology

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“…They also demonstrated differential biofilm presence based upon the anatomical locations of the stent. 14 Becker 8 also noted differential culture growth between proximal and distal stent segments. Murphy et al 16 performed biofilm quantification on the extubated stents and found the mean biomass to be 0.9385 μm 3 /μm 2 (range, 0.3901-1.9511 μm 3 /μm 2 ).…”

Section: Discussionmentioning

confidence: 95%

“…Several studies have demonstrated polymicrobial biofilms on the surface and the lumen of the extubated lacrimal stents. 11–18 Interestingly, biofilms were also demonstrated on the stents obtained from culture-negative patients. 15 Biofilms were demonstrated as early as 3 weeks for Staphylococcus aureus and 4 weeks for P. aeruginosa in in-vitro studies.…”

Section: Discussionmentioning

confidence: 99%

“…[5][6][7][8][9][10][11][12] Few others also assessed the electron microscopic features of stent biofilms regarding their differential locations, quantification, the influence of retention duration, and relation to surgical outcomes. [10][11][12][13][14][15][16][17][18] Culture-dependent studies have demonstrated a culture positivity from 88% to 100% of the stents studied. 5,6 The commonly cultured organisms were Pseudomonas aeruginosa and Staphylococcus aureus, E. coli, and Mycobacterium chelonae.…”

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Microbial Metagenomics of the Extubated Lacrimal Stents Following Dacryocystorhinostomy: The Lacriome Paper 4

Ali

2022

Ophthalmic Plastic &Amp; Reconstructive Surgery

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Purpose: To study the metagenome of the microbes present on the extubated lacrimal stents following a dacryocystorhinostomy. Methods: A prospective study was performed on 10 consecutive extubated lacrimal stents obtained for the metagenomic analysis from the patients following an endoscopic dacryocystorhinostomy. The stents were extubated at 4 weeks postoperatively under endoscopic guidance and immediately transported on ice to the laboratory. Following DNA extraction and library preparation, a whole shotgun metagenome sequencing was performed on the Illumina platform. The downstream processing and bioinformatics of the samples were performed using multiple software packaged in SqueezeMeta pipeline or MG-RAST pipeline. Results: The taxonomic hit distribution across the stent samples showed that bacteria were the most common isolates (mean, 69.70%), followed by viruses (mean, 0.02%) and archaea (0.003%). The 3 major phyla identified were Firmicutes, Actinobacteria, and Proteobacteria. The prevalent organisms include Pseudomonas aeruginosa, Staphylococcus aureus, Corynebacterium accolens, Dolosigranulum pigrum, Citrobacter koserii, Staphylococcus epidermidis, E. coli, and Hemophilus influenza. The functional subsystem profiling demonstrated microbial genes associated with metabolism, cellular, and information processing. The functional subsystem categories were metabolism involving carbohydrates, amino acids, DNA and RNA, cell wall or cell capsule biogenesis, membrane transport, virulence, and defense mechanisms. Conclusions: The present study is the first whole metagenome sequencing of the microbes isolated from the extubated lacrimal stents. The stents harbor diverse microbial communities with distinct ecosystem dynamics. Further studies on microbes-host interactions in the early postoperative period would provide valuable insights.

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Biofilms and Physical Deposits on Nasolacrimal Silastic Stents Following Dacryocystorhinostomy

Abstract: This is the first study to report the differences between ocular and nasal segments of lacrimal stents. These differences could propel further studies on stent biomechanics and their interactions with ocular and nasal tissues, following a dacryocystorhinostomy.

Cited by 15 publications

References 10 publications

Surgical procedure of canaliculoplasty in the treatment of primary canaliculitis associated with canalicular dilatation

Surgical procedure of canaliculoplasty in the treatment of primary canaliculitis associated with canalicular dilatation

Success rate of placement of a bicanalicular stent for partial nasolacrimal obstruction in adults under local, monitored anesthesia care and general anesthesia

Microbial Metagenomics of the Extubated Lacrimal Stents Following Dacryocystorhinostomy: The Lacriome Paper 4

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