Biofilms and their impact on the food industry

Olanbiwoninu, A.A.; Popoola, B.M.

doi:10.1016/j.sjbs.2022.103523

Cited by 28 publications

(8 citation statements)

References 61 publications

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“…In the context of hospital infection control, biofilms formed on medical devices and wound surfaces greatly impact patient health [ 57 ]. Furthermore, within the food industry, microbial biofilms often develop on food packaging, posing risks to food safety [ 58 , 59 ]. Thankfully, numerous studies have reported the antimicrobial biofilm activity of various nanoparticles [ 60 ].…”

Section: Resultsmentioning

confidence: 99%

A potential strategy against clinical carbapenem-resistant Enterobacteriaceae: antimicrobial activity study of sweetener-decorated gold nanoparticles in vitro and in vivo

Liu,

Huang,

Chen

et al. 2023

J Nanobiotechnol

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Background Carbapenem-resistant Enterobacteriaceae (CRE) present substantial challenges to clinical intervention, necessitating the formulation of novel antimicrobial strategies to counteract them. Nanomaterials offer a distinctive avenue for eradicating bacteria by employing mechanisms divergent from traditional antibiotic resistance pathways and exhibiting reduced susceptibility to drug resistance development. Non-caloric artificial sweeteners, commonly utilized in the food sector, such as saccharin, sucralose, acesulfame, and aspartame, possess structures amenable to nanomaterial formation. In this investigation, we synthesized gold nanoparticles decorated with non-caloric artificial sweeteners and evaluated their antimicrobial efficacy against clinical CRE strains. Results Among these, gold nanoparticles decorated with aspartame (ASP_Au NPs) exhibited the most potent antimicrobial effect, displaying minimum inhibitory concentrations ranging from 4 to 16 µg/mL. As a result, ASP_Au NPs were chosen for further experimentation. Elucidation of the antimicrobial mechanism unveiled that ASP_Au NPs substantially elevated bacterial reactive oxygen species (ROS) levels, which dissipated upon ROS scavenger treatment, indicating ROS accumulation within bacteria as the fundamental antimicrobial modality. Furthermore, findings from membrane permeability assessments suggested that ASP_Au NPs may represent a secondary antimicrobial modality via enhancing inner membrane permeability. In addition, experiments involving crystal violet and confocal live/dead staining demonstrated effective suppression of bacterial biofilm formation by ASP_Au NPs. Moreover, ASP_Au NPs demonstrated notable efficacy in the treatment of Galleria mellonella bacterial infection and acute abdominal infection in mice, concurrently mitigating the organism's inflammatory response. Crucially, evaluation of in vivo safety and biocompatibility established that ASP_Au NPs exhibited negligible toxicity at bactericidal concentrations. Conclusions Our results demonstrated that ASP_Au NPs exhibit promise as innovative antimicrobial agents against clinical CRE. Graphical Abstract

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Section: Resultsmentioning

confidence: 99%

A potential strategy against clinical carbapenem-resistant Enterobacteriaceae: antimicrobial activity study of sweetener-decorated gold nanoparticles in vitro and in vivo

Liu,

Huang,

Chen

et al. 2023

J Nanobiotechnol

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“…Additionally, foods of animal origin are also susceptible to contamination throughout the production chain due to cross‐contamination from improper handling practices (e.g., equipment, product handlers, water supply, and so forth) (Flemming, 2016; Olanbiwoninu & Popoola, 2023). The conducive environment on food surfaces serves as an ideal site for microbial growth, leading to multi‐species biofilm formation even after cleaning and disinfection processes (Wagner et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Assessment of probiotic intervention for control of biofilm former Escherichia coli isolates of animal‐origin foods

Sharan,

Dhaka,

Bedi

et al. 2024

Journal of Food Safety

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The study investigates the efficacy of lactic acid bacteria (LAB) strains (Lactobacillus rhamnosus and Lactobacillus casei) in combating Escherichia coli biofilms by determining antibacterial, anti‐biofilm activity, auto‐aggregation, and co‐aggregation assay. The study included 115 E. coli isolates from milk (n = 36), chicken meat (n = 33), and chicken eggs (n = 46). Among 115 E. coli isolates, 22.61% were strong biofilm formers. The LAB strain, L. rhamnosus exhibited a 28.47 mm mean antibacterial inhibition zone, an average reduction of 51.22% in biofilm growth, 55.46% auto‐aggregation, and 41.57% co‐aggregation with E. coli. Similarly, L. casei demonstrated a 21.55 mm mean antibacterial inhibition zone, an average reduction of 36.74% in biofilm growth, 45.23% auto‐aggregation, and 38.74% co‐aggregation with E. coli isolates. Both strains individually and in combination demonstrate substantial reductions in biofilm growth, with L. rhamnosus observed to be more effective than L. casei. Scanning electron microscopy provides valuable insights into the structural aspects of the probiotic impact on diminishing E. coli biofilm. Probiotics' ability to auto‐aggregate and co‐aggregate with pathogenic strains serves as an initial screening method for identifying suitable probiotic bacteria. In conclusion, the results underscore the efficacy of specific LAB strains in combating E. coli biofilm formation. This study provides a basis for future investigations into LAB's capacity to mitigate biofilm‐related hurdles and strengthen microbial management protocols within food processing settings or relevant food substrates.

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“…Foodborne biofilms are of particular concern in the food packaging industry, with the prevalence of some pathogens such as L. monocytogenes, E. coli, or spoilage (e.g., P. fluorescens) microbes, all found to be predominantly adherent resilient formers of biofilms on foods and food preparation and surfaces (Olanbiwoninu and Popoola, 2023). In this study, the combined bactericidal effect of carvacrol (100 and 250 ppm) and pre-formed biofilm monolayer of L. plantarum against foodborne pathogens (E. coli and L. L. monocytogenes were performed, enabling the fulfillment of two objectives: selecting the most effective carvacrol concentration against all target strains and, simultaneously, preserving the L. plantarum monolayer viability to enhance the overall antimicrobial effect.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, the lowest concentration of 100 ppm was selected to evaluate if a reduced amount of carvacrol could be utilized in a combined treatment with L. plantarum pre-formed biofilm, to counteract undesirable organoleptic impact and control foodborne pathogens and food spoilage biofilm (Hellebois et al, 2020;Pateiro et al, 2021). Indeed, antimicrobial resistance of biofilm-forming cells is a severe threat to the food industry (Esposito and Turku, 2023;Olanbiwoninu and Popoola, 2023). It is well-known that Lactobacillus species produce different exometabolites and biosurfactants with antibiofilm activity and are able to compete with pathogens for nutrients and space with different mechanisms of action (Barzegari et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Lactiplantibacillus plantarum monolayer enhanced bactericidal action of carvacrol: biofilm inhibition of viable foodborne pathogens and spoilage microorganisms

Poscente,

Di Gregorio,

Costanzo

et al. 2023

Front. Microbiol.

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The prevalence of biofilm-associated microorganisms and the increasing use of ready-to-eat fresh products represent the current duality the food industry must address. Innovative and eco-friendly antibiofilm solutions and appropriate microbiological food control systems are urgently needed to improve food quality and safety. This study aimed to investigate the in vitro combined efficacy of carvacrol with a pre-formed biofilm monolayer of the probiotic Lactiplantibacillus plantarum DSM 20174. The antimicrobial activity of carvacrol against both planktonic and sessile cells of foodborne pathogens and spoilage microorganisms, alone or in the presence of the pre-formed biofilm of L. plantarum, was investigated by culture-based methods along with flow cytometry (FCM) to monitor cells' cultivability and viability. The synergistic action of carvacrol and the pre-formed biofilm of L. plantarum was evaluated in the 96-well plates. The results showed that L. plantarum pre-formed biofilm monolayer enhanced the antimicrobial effect of carvacrol determining a bactericidal action while the treatment alone induced the viable but not culturable (VBNC) cell state only. Furthermore, the great efficacy of the combined treatment allowed the application of a lower concentration of carvacrol (100 ppm) to achieve significant damage in cell viability. In conclusion, the incorporation of carvacrol into the L. plantarum pre-formed biofilm represents a promising alternative for an antimicrobial functionalized ready-to-eat packaging.

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Biofilms and their impact on the food industry

Cited by 28 publications

References 61 publications

A potential strategy against clinical carbapenem-resistant Enterobacteriaceae: antimicrobial activity study of sweetener-decorated gold nanoparticles in vitro and in vivo

A potential strategy against clinical carbapenem-resistant Enterobacteriaceae: antimicrobial activity study of sweetener-decorated gold nanoparticles in vitro and in vivo

Assessment of probiotic intervention for control of biofilm former Escherichia coli isolates of animal‐origin foods

Lactiplantibacillus plantarum monolayer enhanced bactericidal action of carvacrol: biofilm inhibition of viable foodborne pathogens and spoilage microorganisms

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