Biofilms and Wounds: An Identification Algorithm and Potential Treatment Options

Percival, Steven L.; Vuotto, Claudia; Donelli, Gianfranco; Lipsky, Benjamin A.

doi:10.1089/wound.2014.0574

Cited by 79 publications

(64 citation statements)

References 44 publications

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“…The diagnosing of a biofilm infection represents an area of grave concern, with identification significantly easier on abiotic surfaces following removal of a medical device than on biotic surfaces. For example, evidencing a biofilm within a chronic wound is complex and presently there is no 'gold standard' for that (Percival et al, 2014c). With this in mind, focus upon diagnostic methods that incorporate routine microbiological procedures with more sophisticated methods that are lowcost, reliable and easily incorporated into routine clinical laboratory testing should be considered.…”

Section: Future Perspectivesmentioning

confidence: 99%

Healthcare-associated infections, medical devices and biofilms: risk, tolerance and control

Percival

Suleman

Vuotto

et al. 2015

Journal of Medical Microbiology

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614

482

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Section: Future Perspectivesmentioning

confidence: 99%

Healthcare-associated infections, medical devices and biofilms: risk, tolerance and control

Percival

Suleman

Vuotto

et al. 2015

Journal of Medical Microbiology

Self Cite

614

482

View full text Add to dashboard Cite

“…and P. aeruginosa, could have a great impact, considering that these opportunistic pathogens are responsible of several chronic infections resistant to the most common conventional antibiotics (Percival et al 2015). A previous study demonstrated that a combined formulation of human Tb4 and silver sulfadiazine resulted in a more rapid healing of acute wound compared to the single activities of both compounds (Suman et al 2012).…”

Section: Discussionmentioning

confidence: 99%

A peptide from human β thymosin as a platform for the development of new anti-biofilm agents for Staphylococcus spp. and Pseudomonas aeruginosa

Schillaci

Spinello

Cusimano

et al. 2016

World J Microbiol Biotechnol

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Conventional antibiotics might fail in the treatment of biofilm-associated infections causing infection recurrence and chronicity. The search for antimicrobial peptides has been performed with the aim to discover novel anti-infective agents active on pathogens in both planktonic and biofilm associated forms. The fragment 9-19 of human thymosin β4 was studied through 1 μs MD simulation. Two main conformations of the peptide were detected, both constituted by a central hydrophobic core and by the presence of peripheral charged residues suggesting a possible mechanism of interaction with two models of biological membranes, related to eukaryotic or bacterial membrane respectively. In addition, the peptide was chemically synthesized and its antimicrobial activity was tested in vitro against planktonic and biofilm form of a group of reference strains of Staphylococcus spp. and one P. aeruginosa strain. The human thymosin β4 fragment EIEKFDKSKLK showed antibacterial activity against staphylococcal strains and Pseudomonas aeruginosa ATCC 15442 at concentrations from 12.5 to 6.2 mg/ml and inhibited biofilm formation at sub-inhibitory concentrations (3.1-0.75 mg/ml). The activity of the fragment in inhibiting biofilm formation, could be due to the conformations highlighted by the MD simulations, suggesting its interaction with the bacterial membrane. Human thymosin β4 fragment can be considered a promising lead compound to develop novel synthetic or recombinant derivatives with improved pharmaceutical potential.

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“…Bacterial resistance to silver is rare 78 . Hydrofiber dressings impregnated with silver are absorptive and antibacterial, which will assist in managing the drainage at the G tube site while healing the skin 79 – 88 . There are concerns regarding the absorption of silver into the skin of pediatric patients.…”

Section: Skin Complications Associated With Gastrostomy Tubesmentioning

confidence: 99%

Pediatric Enteral Access Device Management

2016

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Enteral nutrition (EN) support has proven to be a nutrition intervention that can provide full or partial calories to promote growth and development in infants and children. To supply these nutrients, an enteral access device is required, and the use of these devices is growing. Placement of the proper device for the patient need, along with appropriate care and monitoring, is required for individualized patient management. When complications arise, early identification and management can prevent more serious morbidity. Complication management requires a tiered approach starting with staff nurses and ending with a physician expert. In addition to this, each institution needs to have an approach that is coordinated among disciplines and departments to promote consistency of practice. The formation of an enteral access team is a conduit for clinical experts to provide education to families, patients, and healthcare professionals while serving as a platform to address product and practice issues.

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Biofilms and Wounds: An Identification Algorithm and Potential Treatment Options

Cited by 79 publications

References 44 publications

Healthcare-associated infections, medical devices and biofilms: risk, tolerance and control

Healthcare-associated infections, medical devices and biofilms: risk, tolerance and control

A peptide from human β thymosin as a platform for the development of new anti-biofilm agents for Staphylococcus spp. and Pseudomonas aeruginosa

Pediatric Enteral Access Device Management

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