Biofilms of Lactobacillus plantarum and Lactobacillus fermentum: Effect on stress responses, antagonistic effects on pathogen growth and immunomodulatory properties

Aoudia, Nabil; Rieu, Aurélie; Briandet, Romain; Deschamps, Julien; Chluba, Johanna; Jégo, Gaëtan; Guzzo, Jean

doi:10.1016/j.fm.2015.04.009

Cited by 147 publications

(95 citation statements)

References 47 publications

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“…Although strain-specific, biofilm culture is associated with some of the beneficial properties that characterize probiotic bacteria. For example, biofilms are resistant to gastrointestinal environment-related conditions and produce extracellular factors that possess both immunomodulatory properties and the ability to inhibit the growth of pathogens (Rieu et al 2014;Aoudia et al 2016). Studies on the ability of microbial exopolysaccharides to form biofilm have been carried out, although with conflicting results.…”

Section: Exopolysaccharides-host Interactionsmentioning

confidence: 99%

Exopolysaccharides produced by lactic acid bacteria: from health-promoting benefits to stress tolerance mechanisms

Caggianiello

Kleerebezem

Spano

2016

Appl Microbiol Biotechnol

316

181

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A wide range of lactic acid bacteria (LAB) is able to produce capsular or extracellular polysaccharides, with various chemical compositions and properties. Polysaccharides produced by LAB alter the rheological properties of the matrix in which they are dispersed, leading to typically viscous and "ropy" products. Polysaccharides are involved in several mechanisms such as prebiosis and probiosis, tolerance to stress associated to food process, and technological properties of food. In this paper, we summarize the beneficial properties of exopolysaccharides (EPS) produced by LAB with particular attention to prebiotic properties and to the effect of exopolysaccharides on the LAB-host interaction mechanisms, such as bacterial tolerance to gastrointestinal tract conditions, ability of ESP-producing probiotics to adhere to intestinal epithelium, their immune-modulatory activity, and their role in biofilm formation. The pro-technological aspect of exopolysaccharides is discussed, focusing on advantageous applications of EPS in the food industry, i.e., yogurt and gluten-free bakery products, since it was found that these microbial biopolymers positively affect the texture of foods. Finally, the involvement of EPS in tolerance to stress conditions that are commonly encountered in fermented beverages such as wine is discussed.

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Section: Exopolysaccharides-host Interactionsmentioning

confidence: 99%

Exopolysaccharides produced by lactic acid bacteria: from health-promoting benefits to stress tolerance mechanisms

Caggianiello

Kleerebezem

Spano

2016

Appl Microbiol Biotechnol

316

181

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“…Therefore, we expect that strain dependent properties play unmistakable roles also in biofi lm formation. This concept was proposed also by other authors who studied biofi lm formation on polystyrene microplate (AOUDIA et al, 2016).…”

Section: Probiotic Biofi Lm Formation On Carrier Surfacementioning

confidence: 68%

“…Although biofi lm formation on different carriers is well known in the case of pathogenic or commensal microorganisms, probiotic strains have not yet been subjected to study for biofi lm formation, especially on free carriers. There are some studies focusing on biofi lm formation on polystyrene microplates (AOUDIA et al, 2016), but silica and other here presented materials have not been tested yet.…”

Section: Probiotic Biofi Lm Formation On Carrier Surfacementioning

confidence: 99%

Probiotic biofilm on carrier surface: A novel promising application for food industry

Grossová¹,

Rysavka

Márová

2017

Acta Alimentaria

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In this essay work, the ability of probiotic biofi lm formation on carrier surface was demonstrated. Probiotic biofi lms exhibit the same properties as pathogen microbial biofi lms but with higher resistance to low pH values and bile salts. The ability of different probiotic strains (Lactobacillus acidophilus, Bifi dobacterium breve, Bifi dobacterium longum) to interact with pre-selected carriers divided into 3 categories (polymers, complex food matrices, and inorganic compounds) was tested. Lactobacillus acidophilus and Bifi dobacterium longum combined with inorganic silica carrier exhibited the interaction leading to biofi lm formation only. Prepared biofi lm (Lactobacillus acidophilus) was then subjected to comparative study with planktonic bacterial culture. The ability to survive in the presence of low pH value (pH 1-3) and bile salts (0.3% solution) was evaluated. Low pH value (pH 1) had a harsh effect on free cell culture causing decreased cell viability (71.9±3.2% of viable cells). Biofi lm culture exhibited higher resistance to low pH value, the viability exceeded 90%. The exposure of free cell probiotic culture to porcine bile resulted in an almost constant decrease in viability during the study period (68.2±1.1% of viable cells, after 240 min incubation). Viability of biofi lm after the exposition to bile was almost constant with a slight decrease of no more than 5% during the study.Keywords: probiotics, biofi lm, carrier, acid tolerance, bile toleranceThe fi rst mention of probiotics appeared at the beginning of the 20 th century in the writings of Elie Metchnikoff. He suggested that the longevity and healthy life of Bulgarian people is hidden in their consumption of fermented milk products (TRIPATHI & GIRI, 2014). In 2001, probiotics as "live microorganisms, which when administered in adequate amounts confer a health benefi t on the host" (FAO/WHO, 2001) was defi ned.During the last 15-20 years, it was recognized that the developmental process of biofi lm formation is the natural way of life for microorganisms, without differences between harmful and commensal microorganisms. Therefore, on one hand, biofi lm formation is an important clinical pathogenic mechanism, a menace to the aging population, immunocompromised and poly-traumatic patients, on the other hand, a modern medical instrumental intervention (RÖMLING et al., 2014). In the common line, biofi lm cells have a higher resistance to antimicrobial agents and agents harmful to microbes in biofi lm than planktonic bacteria. The formation of a barrier or biofi lm prohibits the direct contact with harmful agents (SREY et al., 2013). * To whom correspondence should be addressed. Phone: +420 736 468 302; e-mail: grossova@favea.czThis is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 License, which permits unrestricted use, distribution, and reproduction in any medium for non-commercial purposes, provided the original author and source are credited.

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“…Inhibitory mechanisms of probiotic Lactobacillus strains on biofilm formation of L. monocytogenes based on the competition, exclusion and displacement [21] . Similarly, Aoudia et al [20] reported that biofilm growth in probiotic Lactobacillus strains had an antagonistic effect against L. monocytogenes. Similarly, many researchers concluded that probiotic Lactobacillus strains or lactobacilli was capable to reduce biofilm formation of L. monocytogenes [4,21,27,28] .…”

Section: Discussionmentioning

confidence: 96%

“…The use of probiotic biofilms can be considered as an alternative approach for reducing growth of pathogenic bacteria as regards human health and food safety [4] . However, the optimal functionality and expression of health-promoting physiological functions of probiotics is dependent on survivability and colonization in gastrointestinal tract and fermented foods [20] . Transition from planktonic cells to biofilm of the most bacteria depends on bacterial community and environmental conditions [21] .…”

Section: Introductionmentioning

confidence: 99%

Listeria monocytogenes’in Gelişimi Üzerine Probiyotik Biyofilmlerin İnaktivasyon Etkisi

Turhan¹,

Erginkaya²,

Uney³

et al. 2017

Kafkas Univ Vet Fak Derg

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Probiotic lactic acid bacteria and their biofilms have antagonistic activity against food spoilage organisms and pathogenic bacteria. Recently, researchers focused on the use of probiotic biofilms for inhibition of pathogenic bacteria. The aim of this research is to improve probiotic biofilms with optimal prebiotic concentration and to determine their inactivation effect on both planktonic cells and biofilm growth of Listeria monocytogenes. Biofilm formations were detected by using microplate method. Prebiotic ingredients were used to form biofilm with highest viable probiotic cell counts and optimal concentrations of prebiotic ingredients were determined according to the response surface method. Biofilm produced by Lactobacillus casei Shirota and Lactobacillus rhamnosus contained 9.46 and 9.66 log cfu/mL viable cell counts, respectively. Optimal prebiotic concentrations were found 3% casein peptone-0% fructo-oligosaccharides (FOS) for biofilm formation with highest viable cell counts by L. casei Shirota and 1.5% casein peptone-1.5% FOS for biofilm formation with highest viable cell counts by L. rhamnosus. Probiotic biofilms exhibited inactivation against growth of L. monocytogenes and caused a reduction of 0.66-2.01 log cfu/mL for planktonic L. monocytogenes and 0.40-1.69 log cfu/mL for L. monocytogenes biofilm. Planktonic cells of L. monocytogenes were observed to be more susceptible to probiotic biofilms than biofilm of L. monocytogenes. Biofilm of L. rhamnosus showed higher inhibition effect on L. monocytogenes growth than L. casei Shirota. These findings showed that biofilms of probiotic Lactobacillus strains used in this study may be excellent candidate for controlling of pathogenic bacteria.

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Biofilms of Lactobacillus plantarum and Lactobacillus fermentum: Effect on stress responses, antagonistic effects on pathogen growth and immunomodulatory properties

Cited by 147 publications

References 47 publications

Exopolysaccharides produced by lactic acid bacteria: from health-promoting benefits to stress tolerance mechanisms

Exopolysaccharides produced by lactic acid bacteria: from health-promoting benefits to stress tolerance mechanisms

Probiotic biofilm on carrier surface: A novel promising application for food industry

Listeria monocytogenes’in Gelişimi Üzerine Probiyotik Biyofilmlerin İnaktivasyon Etkisi

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