Biofilms on Meat Processing Surfaces

Chamberlain, Anne; Johal, S.

doi:10.1007/978-94-009-1363-9_8

Cited by 9 publications

(7 citation statements)

References 2 publications

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“…to inert surfaces are not completely understood; several studies have shown that adhesion of bacteria partly depends upon the nature of the inert surfaces and partly upon the bacterial surface properties (7,9,19). Hydrophobicity and surface charge are the most important surface properties in the adhesion process as demonstrated by innumerous studies (18,25,27,32,36).…”

Section: Introductionmentioning

confidence: 99%

Adhesion of Salmonella Enteritidis to stainless steel surfaces

Oliveira¹,

Oliveira

Teixeira

et al. 2007

Braz. J. Microbiol.

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Adhesion of microorganisms to food processing surfaces and the problems it causes are a matter of strong concern to the food industry. Contaminated food processing surfaces may act as potential sources of transmission of pathogens in food industry, catering and in the domestic environments. Several studies have shown that adhesion of bacteria to surfaces partly depends upon the nature of the inert surfaces and partly upon the bacterial surface properties. The aim of this study was to compare the adhesion of four different strains of Salmonella Enteritidis to stainless steel 304 (SS 304). The effect of surface hydrophobicity and surface elemental composition on the adhesion process was also analysed. Hydrophobicity was evaluated through contact angle measurements using the sessile drop method. All the strains studied showed positive values of the degree of hydrophobicity (ΔGlwl) and so can be considered hydrophilic while stainless steel revealed a hydrophobic character. Bacterial cell surface composition was measured using X-ray photoelectron spectroscopy (XPS). The XPS results corroborated the similarity of the values of the degree of hydrophobicity obtained by contact angles. The different Salmonella strains showed similar elemental composition and cell surface physico-chemical properties. Nevertheless, S. Enteritidis MUSC presented higher adhesion ability to SS 304 (p<0.05). It can be concluded that the physico-chemical properties of the strain does not explain the ability of adhesion to stainless steel. Other factors like the production of polysaccharides must be considered.

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Section: Introductionmentioning

confidence: 99%

Adhesion of Salmonella Enteritidis to stainless steel surfaces

Oliveira¹,

Oliveira

Teixeira

et al. 2007

Braz. J. Microbiol.

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“…The factors governing the adhesion of bacteria to surfaces are still not well understood. In several studies, the adhesion of bacteria partly depended on both the nature of the inert surfaces and the properties of the bacterial surface (8,11,16,42,45). Nevertheless, in some situation it has been difficult to establish a relationship between surface properties and the extent of bacterial adhesion (10,30,31,33).…”

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confidence: 99%

Adhesion to and Viability of Listeria monocytogenes on Food Contact Surfaces

Silva

Teixeira

Oliveira

et al. 2008

Journal of Food Protection

137

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Listeria monocytogenes is an important pathogen responsible for major outbreaks associated with food products. Adhesion to surfaces leads to significant modifications in cell physiology. The aim of this work was to determine the adhesion ability of 10 isolates of L. monocytogenes to eight materials commonly used in kitchens and to evaluate the viability of the adhered cells. The materials assayed were stainless steel 304, marble, granite, glass, polypropylene from a bowl and from a cutting board, and two kinds of silestone. All L. monocytogenes strains attached to all surfaces, although to different extents. L. monocytogenes adhered most tightly to granite and marble, followed by stainless steel 304, glass, silestones, and finally polypropylene surfaces. Surfaces at the threshold between hydrophobicity and hydrophilicity, with high electron acceptor capability and a regular pattern of roughness, were more prone to attachment. Polypropylene surfaces displayed the highest percentage of viable bacteria (nearly 100%), whereas marble and granite had a lower percentage of cultivable cells, 69.5 and 78.7%, respectively. The lowest percentage of culturable bacteria was found on white silestone (18.5%). These results indicate that there are differences in adhered cell viability on different materials. Cell viability assays are important to better understand the cross-contamination process because only adhered bacteria that remain viable are responsible for postprocess contamination.

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“…Several studies have shown that adhesion of bacteria partly depends upon the nature of the inert surfaces and partly upon the bacterial surface properties (Carballo et al, 1992;Chamberlain and Johal, 1988a;Hood and Zottola, 1995). Hydrophobicity and surface charge are the most important surface properties in the adhesion process as demonstrated in numerous studies (Kalmokoff et al, 2001;Monica et al, 2003;Oliveira et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…According to Vogler's criterion (Vogler, 1998) the materials tested here are classified as hydrophobic with the exception of glass, which are considered hydrophilic (Cox et al, 1989;Mafu et al, 1991). SS was sometimes classified as hydrophilic (Chamberlain and Johal, 1988b;Leclercq-Perlat and Lalande, 1994;Mafu et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

Adhesive ability and biofilm metabolic activity of Listeria monocytogenes strains before and after cold stress

SLAMA¹

2012

Afr. J. Biotechnol.

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Listeria monocytogenes is an important pathogen responsible for major outbreaks associated with food products. Adhesion to surfaces leads to significant modifications in cell physiology. In this work, the ability of L. monocytogenes to produce biofilm and its ability to adhere to abiotic surfaces under cold stress were evaluated. Metabolic activity of biofilm formed by L. monocytogenes before and after cold stress was measured in vitro using the colorimetric method based on the reduction of the tetrazolium salt 2,3-bis(2methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT). The ability to adhere to abiotic surfaces was determined by the ability of the cells to metabolically reduce bromure de 3-[4,5dimethylthiazol-2-yl]-2,5-diphenyltetrazolium (MTT) to a formazan dye. Our results show that L. monocytogenes strains were able to adhere to abiotic materials with different degrees. In fact, cold stressed strains (-20°C) were more adhesive to polyethylene, glass, polyvinyl chloride and stainless style surfaces than non-stressed cells. Our observations show that the hydrophily varied with cold stress period. At freezing temperature, L. monocytogenes was strongly hydrophobic. Genetic studies of adhesive genes of L. monocytogenes will be required to fully understand the importance of this observation.

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Biofilms on Meat Processing Surfaces

Cited by 9 publications

References 2 publications

Adhesion of Salmonella Enteritidis to stainless steel surfaces

Adhesion of Salmonella Enteritidis to stainless steel surfaces

Adhesion to and Viability of Listeria monocytogenes on Food Contact Surfaces

Adhesive ability and biofilm metabolic activity of Listeria monocytogenes strains before and after cold stress

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