Step tracer tests were carried out on lab-scale biofilters to determine the residence time distributions (RTDs) of gases passing through two types of biofilters: a standard biofilter with vertical gas flow and a modified biofilter with horizontal gas flow. Results were used to define the flow patterns in the reactors. "Non-ideal flow" indicates that the flow reactors did not behave like either type of ideal reactor: the perfectly stirred reactor [often called a "continuously stirred tank reactor" (CSTR)] or the plug-flow reactor.The horizontal biofilter with back-mixing was able to accommodate a shorter residence time without the usual requirement of greater biofilter surface area for increased biofiltration efficiency. Experimental results indicated that the first bed of the modified biofilter behaved like two CSTRs in series, while the second bed may be represented by two or three CSTRs in series. Because of the flow baffles used in the horizontal biofilter system, its performance was more similar to completely mixed systems, and hence, it could not be modeled as a plug-flow reactor. For the standard biofilter, the number of CSTRs was found to be between 2 and 9 depending on the airflow rate. In terms of NH 3 removal efficiency and elimination capacity, the standard biofilter was not as good as the modified system; moreover, the second bed of the modified biofilter exhibited greater removal efficiency IMPLICATIONS Models of biofilter operation that are developed without an understanding of the non-ideal flow of reactants in a biofilter would be of limited use for design purposes. In order to understand why a specific reactor can appear to have longer or shorter reaction times than predicted, it is necessary to have a model of the rate at which odorous gases will be oxidized, and to consider the residence time distribution of the reactants in the biofilter. This paper describes the use of tracer studies to determine the residence time distribution, to characterize the flow behavior of the reactant in a multistage biofilter, and to explain the different performances associated with a standard and a modified biofilter system.