BioFire FilmArray BCID2 versus VITEK-2 System in Determining Microbial Etiology and Antibiotic-Resistant Genes of Pathogens Recovered from Central Line-Associated Bloodstream Infections

Abstract

Central line-associated bloodstream infection (CLABSI) is among the most serious hospital acquired infections. Therefore, the rapid detection of the causative microorganism is of crucial importance to allow for the appropriate antimicrobial therapy. In the present study, we analyzed the clinical performance of the BioFire FilmArray Blood Culture Identification 2 (BCID2) panel in the identification of 33 microbial species and 10 antibiotic resistance genes in comparison to the VITEK-2 system. A total of 104 blood specimens were included. The FilmArray BCID2 results were concordant with the VITEK-2 system in 69/97 specimens (71.1%). Non-concordance was either due to the detection of more pathogens by the FilmArray BCID2 23/28 (82%) or microbial species were misidentified 5/28 (18%). Hence, in comparison to the VITEK-2 system, the FilmArray BCID2 panel showed an overall sensitivity of 75.8% (95% CI, 66–83%) and an overall specificity of 98% (95% CI, 97–98.8%) in detecting microbial species. For the resistance genes, the FilmArray BCID was able to detect the presence of blaCTX-M gene in 23 Gram-negative isolates, blaNDM and blaOXA-48- like genes in 14 and 13 isolates, respectively. The mecA and mecC genes were found in 23 Staphylococcus species, while mecA, mecC and MREJ genes were found in 4 Staphylococcus aureus isolates. The sensitivity and specificity for detecting resistance genes by the FilmArray BCID2 was 90% (95% CI, 81.4–95%) and 99.6% (95% CI, 99–100%), respectively. As concluded, the present study emphasizes the high sensitivity and specificity of the FilmArray BCID2 in the rapid and reliable detection of different bacteria and fungi from positive blood culture bottles, as well as the accurate detection of various antibiotic resistance markers.

Antimicrobial Stewardship on Patients with Neutropenia: A Narrative Review Commissioned by Microorganisms

“…However, these PCR assays are limited by the primers’ availability. The BioFireFilmArray BCID2 assay identifies 33 organisms by multiplex PCR from positive blood cultures in one hour, and detects 10 resistance markers [ 63 , 64 ].…”

Detection of Common Bacterial Causes of Otitis in Dogs in Mosul City, Iraq

“…The VITEK 2 System is a new diagnostic technique that could be used for bacterial identification, and also it can detect bacterial sensitivity for antimicrobials. This automatic system relies on the fluorescence-based techniques for diagnosis [16,17]. The results of this system can be obtained within 1-3 hours for bacterial identification, and up to 18 hours for antimicrobial sensitivity testing [16].…”

Microbiological Assessment of the FilmArray Blood Culture Identification 2 Panel: Potential Impact in Critically Ill Patients

“…In detail, the new BioFire FilmArray BCID2 panel analyzes a total of 43 targets associated with BSI, including 15 Gram-negative bacteria, 11 Gram-positive bacteria, 7 yeast species, and 10 antimicrobial resistance genes including those encoding carbapenemases (IMP, KPC, OXA-48-like, NDM, and VIM), and CTX-M-type extended-spectrum β-lactamases (ESBLs), as well as those responsible for colistin resistance ( mcr-1 ), methicillin resistance ( mecA/C, and specifically for methicillin-resistant Staphylococcus aureus —MRSA, mecA/C and MREJ— mec right-extremity junction) and vancomycin resistance ( vanA/B ) [ 8 ]. Unlike BCID, only a few published studies are available in the literature to validate and support BCID2 panel potential functioning and implementation in BSI routine workflows [ 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 ].…”