Bioflocculant produced by Chlamydomonas reinhardtii

Abstract

Bioflocculants of Chlamydomonas reinhardtii were investigated under axenic conditions. C. reinhardtii was found to produce significant amounts of bioflocculants. Flocculating activity by C. reinhardtii began in the linear phase of growth and continued until the end of the stationary phase. The highest flocculating efficiency of the culture broth was 97.06%. The purified C. reinhardtii bioflocculant was composed of 42.1% (w/w) proteins, 48.3% carbohydrates, 8.7% lipids, and 0.01% nucleic acid. The optimum condition for bioflocculant production of C. reinhardtii was as follows: under temperature of 15°C to 25°C, pH 6-10 and illumination of 40-60 μmol photons m −2 s −1 . The bioflocculants produced by C. reinhardtii showed maximum activity in pH ranges from 2 to 10. The flocculating activity was significantly enhanced by the addition of CaCl 2 as a coflocculant at an optimal concentration of 4.5 mM.

A Mechanistic View of the Light-Induced Synthesis of Silver Nanoparticles Using Extracellular Polymeric Substances of Chlamydomonas reinhardtii

“…The major functional groups in C. reinhardtii EPS, measured by FTIR in our previous work, are presented in Table 1 [13]. These functional groups represent of polysaccharides, polyphenols and proteins, corroborating previous studies [26,27]. Table 1 shows the availability of functional groups known to donate electrons, such as OH − [17,19,28], that reduce various metal ions to their corresponding metallic counterparts, as well as functional groups that can stabilize the NPs.…”

Studies on Bioflocculant Production by Bacillus sp. AEMREG7

“…SM9913 was optimal at pH 7 [54]. Gong et al [21] reported that the crude bioflocculant produced by Serratia ficaris flocculated well at pH 5-7, whereas Gao et al [10] and Zhu et al [55] found that the flocculating activity of bioflocculants produced by Rothia sp. and Chlamydomonas reinhardtii were maximal at pH 9 and 10, respectively.…”

Sludge settling and algal flocculating activity of extracellular polymeric substance (EPS) derived from bacillus cereus SK

“…Bioflocculant produced by Chlamydomonas reinhardtii composed of 42.1% (w / w) proteins, 48.3% carbohydrates, 8.7% lipids & 0.01% nucleic acid and exhibited kaolin clay flocculating efficiency of 97.06% (Zhu et al . ). Among the fungi, Solibacillus silvestris produced proteoglycan bioflocculant with a potential to harvest marine microalga, Nannochloropsis oceanic for cost‐effective production of microalgal bioproducts (Wan et al .…”