Biofluid Collection in Metabolomics by the Application of the novel Volumetric Absorptive Microsampling Technology: a mini-Review

Meesters, Roland J.W.

doi:10.17145/rss.19.004

Cited by 4 publications

(2 citation statements)

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“…Due to a heterogeneous distribution of the blood or to chromatographic processes of the drugs with the cellulose, the distribution of the analytes can be heterogeneous inside the blood spot [11,12]. Although the mechanisms behind the nonhomogeneous distribution are yet to be understood, it is obvious that under this situation, the hematocrit and the punching position become critical factors for quantitative analysis and should be carefully studied.…”

Section: Resultsmentioning

confidence: 99%

“…The former requires an accurate measurement of the volume placed in the card if a quantitative analysis is to be performed [10,11]. Fortunately, some solutions for its simple measurement have been developed, such as volumetric absorptive microsampling (VAMS) [12]. However, some of these methodologies can be more expensive or require appropriate infractructures in comparison to “traditional” DBS [13].…”

Section: Introductionmentioning

confidence: 99%

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Analysis of the Heterogeneous Distribution of Amiloride and Propranolol in Dried Blood Spot by UHPLC-FLD and MALDI-IMS

et al. 2019

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Dried blood spot (DBS) has lately experienced an increase in its use in bioanalysis due to its several advantages compared with traditional blood sampling methods. Nevertheless, the use of DBS with quantitative purposes is hindered by the heterogeneous distribution of some compounds in the supporting matrix and the dependence of the response on different factors, such as the hematocrit, blood volume, and sampling position. In this study the effect of those factors in the analytical response was investigated by ultra high performance liquid chromatography coupled to fluorescence detection, using amiloride and propranolol as model compounds. The results showed a heterogeneous and drug-dependent distribution of the compounds in the blood spot. While amiloride concentration was higher in the center, propranolol concentration was higher in the periphery of the spot. Besides, the influence of the hematocrit on the quantitative results was observed. MALDI mass spectrometry imaging (MALDI-IMS) has allowed study of the distribution of the two cardiovascular drugs when they were placed in the DBS card using water:methanol solutions, demonstrating that they followed a similar distribution pattern as in blood. This work has showed the potentiality of the MALDI-IMS technique to predict the distribution of the drugs in the DBS card.

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Section: Resultsmentioning

confidence: 99%