Biofluidic Random Laser Cytometer for Biophysical Phenotyping of Cell Suspensions

He, Jijun; Hu, Shuhuan; Ren, Jifeng; Cheng, Xin; Hu, Zhenjiang; Wang, Ning; Zhang, Huangui; Lam, Raymond H. W.; Tam, Hwa Yaw

doi:10.1021/acssensors.8b01188

Cited by 31 publications

(24 citation statements)

References 48 publications

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“…As a new type of laser, random lasers utilize multiple scattering to achieve stimulated radiation amplification, avoiding the complex manufacturing process and high cost of conventional resonators [6][7][8][9][10][11][12] . Random lasers have becoming a hot research field in the international laser science community due to their special characteristics of low cost, small size and ease of integration, demonstrating versatile application prospects in bio-detection 13,14 , information security 15 , and integrated photoelectron 16 . More importantly, the low spatial coherence of random lasing naturally makes up for the shortcomings of traditional lasers in full-field imaging and display.…”

Section: Introductionmentioning

confidence: 99%

A ring-shaped random laser in momentum space

Bian

Shi

et al. 2020

Nanoscale

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A color-switchable random laser is designed through directly coupling random laser with a commercial optical fiber. By using a simple approach of selectively coating the random gain layer on the surface of fiber, the red and yellow random lasers are respectively achieved with low threshold and good emission direction due to the guiding role of optical fibers. Moreover, the unique coupling mechanism leads to the random lasing with ring-shape in momentum space, indicating an excellent illuminating source for high-quality imaging with an extremely low speckle noise. More importantly, random lasing with different colors can be flexible obtained by simply moving the pump position. The results may promote random lasers' practical applications in the fields of sensing, in vivo biologic imaging, high brightness full-field illuminating. Experimental MethodsThe fabrication process of the fiber source is illustrated in Fig. 1a. The typical gain materials used in this experiment are: 4-(Dicyanomethylene)-2-tert-butyl-6-(1,1,7,7-tetramethyljulolidin-4-yl-vinyl) -4H-pyran(DCJTB, Tokyi Chemical Industry) and Pyrromethene567 (PM567, Sigma). The fabricated process of the fiber source is simply supplied as follows. First, polydimethylsiloxane (PDMS, n = 1.41) solution is mixed with cross-linking solution with the ratio of 1:10. Then, TiO 2 nanoparticles are dispersed in the dye-doped acetone solution (DCJTB at 1.5 mg mL -1 or PM567 at 1.25 mg mL -1 ) to obtain TiO 2 dispersion with a concentration of 0.9 mg mL -1 . The dye-doped TiO 2 dispersion and PDMS are mixed at a volume ratio of 1: 5 in an ultrasonic tank for 15 min and then vacuum them for 40 min to remove the air bubble. Finally, the mixture is dipped onto a clean fiber (TAIHAN Fiber Optics) to flow naturally. The length of the fiber is about 60 mm, the core diameter is 50 μm and the diameter of the cladding is 125 μm. The refractive indexes of the core and cladding layer are 1.54 and 1.52, respectively. The sample is placed in a drying oven at 80 °C for 3 hours to complete the cross-linking polymerization and drying. After cooling to room temperature, a fiber source is realized. Random lasers with different colors can be fabricated by coating different dye polymers at different locations on the surface of the fiber. And the polymer film with different thicknesses can be fabricated by controlling the dipping times.Acs Nano, 2017, 11. 36.

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Section: Introductionmentioning

confidence: 99%

A ring-shaped random laser in momentum space

Bian

Shi

et al. 2020

Nanoscale

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“…Random lasers (RLs), with optical feedback from light scattering rather than a resonator, have been extensively investigated in the past decade and the applications of RLs for specklefree imaging, cell analysis, and tumor tissue detection were demonstrated. [154][155][156][157] The multiple scattering from appropriate scatterers (e.g., nanoparticles) is sometimes strong enough to offer optical resonance for lasing, which determines the lasing characteristics. The RLs usually have comparatively low Q-factor The SEM images of a hollow core PBG fiber.…”

Section: Optical Resonance Based On Random Scatteringmentioning

confidence: 99%

Fiber Optofluidic Microlasers: Structures, Characteristics, and Applications

Yang

Gong

Zhang

et al. 2021

Laser & Photonics Reviews

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Herein, recent progress in fiber optofluidic lasers and their applications in biochemical detection are reviewed. The unique properties of optical fibers are introduced for the development of optofluidic microlasers, including high quality factor, small mode volume, high aspect ratio, high reproducibility, and low cost. Optical fiber microresonators based on Fabry-Pérot cavity, microring resonator or photonic bandgap cavity are highlighted to provide optical feedback for optofluidic lasing. For applications, the capability of fiber optofluidic lasers is emphasized to perform biochemical analysis with ultrahigh sensitivity, fast response, high throughput, and disposable optical biodetection. The challenges and prospects for fiber optofluidic lasers are also discussed.

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“…Indeed, random lasing has been reported in biological tissues infiltrated by dye molecules, such as animal tissue [ 59 ], bone [ 60 ], and the wings of insects [ 61 , 62 ]. Moreover, ex vivo cancerous tissue, once infiltrated by a gain material, has been reported as suitable to generate random lasing, opening new opportunities in diagnostics [ 63 , 64 , 65 ] and opto-chemical therapies [ 66 ]. In fact, malignant tissue shows a different spectral signature in the random laser emission compared to a healthy one, due to differences in the microstructure.…”

Section: Introductionmentioning

confidence: 99%

Direct Measurement of the Reduced Scattering Coefficient by a Calibrated Random Laser Sensor

Tommasi

Auvity

Fini

et al. 2022

Sensors

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The research in optical sensors has been largely encouraged by the demand for low-cost and less or non-invasive new detection strategies. The invention of the random laser has opened a new frontier in optics, providing also the opportunity to explore new possibilities in the field of sensing, besides several different and peculiar phenomena. The main advantage in exploiting the physical principle of the random laser in optical sensors is due to the presence of the stimulated emission mechanism, which allows amplification and spectral modification of the signal. Here, we present a step forward in the exploitation of this optical phenomenon by a revisitation of a previous experimental setup, as well as the measurement method, in particular to mitigate the instability of the results due to shot-to-shot pump energy fluctuations. In particular, the main novelties of the setup are the use of optical fibers, a reference sensor, and a peristaltic pump. These improvements are devoted to: eliminating optical beam alignment issues; improving portability; mitigating the variation in pump energy and gain medium performances over time; realizing an easy and rapid change of the sensed medium. The results showed that such a setup can be considered a prototype for a portable device for directly measuring the scattering of liquid samples, without resorting to complicated numerical or analytic inversion procedures of the measured data, once the suitable calibration of the system is performed.

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Biofluidic Random Laser Cytometer for Biophysical Phenotyping of Cell Suspensions

Cited by 31 publications

References 48 publications

A ring-shaped random laser in momentum space

A ring-shaped random laser in momentum space

Fiber Optofluidic Microlasers: Structures, Characteristics, and Applications

Direct Measurement of the Reduced Scattering Coefficient by a Calibrated Random Laser Sensor

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