Biofuel Cell Based on Anode and Cathode Modified by Glucose Oxidase

Krikstolaityte, Vida; Öztekin, Yasemin; Kuliesius, Jurgis; Ramanavičienė, Almira; Yazicigil, Zafer; Ersöz, Mustafa; Okumuş, Aytuğ; Kausaitė-Minkstimienė, Asta; Kılıç, Zeynel; Solak, Alı Osman; Makaraviciute, Asta

doi:10.1002/elan.201300482

Cited by 50 publications

(29 citation statements)

References 45 publications

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“…0.6 mA cm −2 at 14 mL min −1 (the maximal power density of 109 µW cm −2 has been achieved at 0.45 V ( Figure S1)). These performances in the fuel cell mode of operation (U cell > 0, i cell > 0) are comparable to performances of other glucose/oxygen enzymatic fuel cells [24][25][26][27][28]. The electroenzymatic reactor in the present study also outperforms significantly other fuel cells based on the same combination of anode and cathode catalysts (e.g., approx.…”

Section: Gox/gox-hrp Reactorsupporting

confidence: 70%

Selectivity and Sustainability of Electroenzymatic Process for Glucose Conversion to Gluconic Acid

et al. 2020

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Electroenzymatic processes are interesting solutions for the development of new processes based on renewable feedstocks, renewable energies, and green catalysts. High-selectivity and sustainability of these processes are usually assumed. In this contribution, these two aspects were studied in more detail. In a membrane-less electroenzymatic reactor, 97% product selectivity at 80% glucose conversion to gluconic acid was determined. With the help of nuclear magnetic resonance spectroscopy, two main side products were identified. The yields of D-arabinose and formic acid can be controlled by the flow rate and the electroenzymatic reactor mode of operation (fuel cell or ion-pumping). The possible pathways for the side product formation have been discussed. The electroenzymatic cathode was found to be responsible for a decrease in selectivity. The choice of the enzymatic catalyst on the cathode side led to 100% selectivity of gluconic acid at somewhat reduced conversion. Furthermore, sustainability of the electroenzymatic process is estimated based on several sustainability indicators. Although some indicators (like Space Time Yield) are favorable for electroenzymatic process, the E-factor of electroenzymatic process has to improve significantly in order to compete with the fermentation process. This can be achieved by an increase of a cycle time and/or enzyme utilization which is currently low.

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Section: Gox/gox-hrp Reactorsupporting

confidence: 70%

Selectivity and Sustainability of Electroenzymatic Process for Glucose Conversion to Gluconic Acid

et al. 2020

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“…This depends mainly on the choice of enzymes used for the cathode side (typically BOD or laccase), and on the choice of the mediator used for GOx regeneration on the anode. The reported values in literature range from 0.45 V for the fuel cell following similar idea as in the present paper but, with phenanthroline as GOx mediator [36] to 0.9 V for the Osmium redox hydrogels as GOx mediator [37,38]. In general, the investigated electrochemical cell shows a high open circuit cell A B Fig.…”

Section: Electrochemical Performancementioning

confidence: 70%

“…The power density of the here presented Reactor 3 can be compared to other glucose/oxygen fuel cells based on GOx and BOD or laccase as cathode [39][40][41][42][43][44]. The performance of our reactor is superior compared to the enzymatic fuel cell with the same combination of enzymes and a maximum power density of around 5 mW cm À2 for 20 mM glucose solution [36]. But this power level is clearly below the performance of the so far reported best performance of enzymatic fuel cells [45].…”

Section: Electrochemical Performancementioning

confidence: 70%

Gluconic Acid Synthesis in an Electroenzymatic Reactor

Varničić

Vidaković‐Koch

Sundmacher

2015

Electrochimica Acta

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A B S T R A C TGlucose was selectively oxidized to gluconic acid in a membraneless, flow-through electroenzymatic reactor operated in the mode of co-generating chemicals and electrical energy. At the anode the enzyme glucose oxidase (GOx) in combination with the redox mediator tetrathiafulvalene (TTF) was used as catalyst, while the cathode was equipped with an enzyme cascade consisting of GOx and horseradish peroxidase (HRP). The influence of the electrode preparation procedure, the structural and the operating parameters on the reactor performance was investigated in detail. Under optimized conditions, an open circuit potential of 0.75 V, a current density of 0.6 mA cm À2 and a power density of 100 mA cm À2 were measured. The space time yield of gluconic acid achieved at a glucose conversion of 47% was 18.2 g h À1 cm À2 .

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“…These issues could be overcome by employing the same biocatalyst for both the anodic and the cathodic reactions driven by the same substrate, hence potentially improving efficiency and cost. There is only one report of a biofuel cell powered by the same substrate 31 . The anodic reaction was based on mediated GOx, whereas the cathodic reaction exploited GOx with peroxide transduction by co-immobilised horseradish peroxidase.…”

Section: Introductionmentioning

confidence: 99%

Cholesterol Self-Powered Biosensor

et al. 2014

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Monitoring the cholesterol level is of great importance, especially for people with high risk of developing heart disease. Here we report on reagentless cholesterol detection in human plasma with a novel single-enzyme, membrane-free, self-powered biosensor, in which both cathodic and anodic bioelectrocatalytic reactions are powered by the same substrate. Cholesterol oxidase was immobilised in a sol-gel matrix on both the cathode and the anode. Hydrogen peroxide, a product of the enzymatic conversion of cholesterol, was electrocatalytically reduced, by the use of Prussian blue, at the cathode. In parallel, cholesterol oxidation catalysed by mediated cholesterol oxidase occurred at the anode. The analytical performance was assessed for both electrode systems separately. The combination of the two electrodes, formed on high surface-area carbon cloth electrodes, resulted in a self-powered biosensor with enhanced sensitivity (26.0 mA M -1 cm -2 ), compared to either of the two individual electrodes, and a dynamic range up to 4.1 mM cholesterol.Reagentless cholesterol detection with both electrochemical systems and with the self-powered biosensor was performed and the results were compared with the standard method of colorimetric cholesterol quantification.

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Biofuel Cell Based on Anode and Cathode Modified by Glucose Oxidase

Cited by 50 publications

References 45 publications

Selectivity and Sustainability of Electroenzymatic Process for Glucose Conversion to Gluconic Acid

Selectivity and Sustainability of Electroenzymatic Process for Glucose Conversion to Gluconic Acid

Gluconic Acid Synthesis in an Electroenzymatic Reactor

Cholesterol Self-Powered Biosensor

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