Biofuel Cell-Driven Robust Electrochemiluminescence Biosensing Platform

Abstract

Electrochemiluminescence (ECL) is one powerful tool in the sensing field, in which the electrochemical workstation is necessary to achieve the electrical/optical signal conversion in the presence of luminescent agents. By contrast, biofuel cells (BFCs) can also provide electricity from renewable biofuels under moderate conditions. More importantly, BFCs with the features of adjustable voltage output and excellent compatibility would well meet the requirement of working voltages for different ECL devices. However, to the best of our knowledge, the BFC-driven luminous system has not been reported. In this work, we constructed, for the first time, a BFC-driven ECL system with fast and stable signal outputs. To demonstrate the proof-of-concept of the BFC− ECL system, the sensitive and selective detection of histidine was achieved, exhibiting great potential among point-of-care diagnoses in remote regions. Overall, this work not only paves a new way for the conversion of chemical energy, electrical energy, and luminous system but also explores the new application of BFC.

A sensitive electrochemiluminescence immunosensor for carbohydrate antigen 12-5 analysis based on dual-function SnO2 nanoflowers

“…Compared with the aforementioned methods, electrochemiluminescence (ECL) immunoassay has been generally applied in environmental, food, and biological detection due to its low background signal, high sensitivity, good selectivity, rapid response, wider dynamic range, and easier operating procedures. [15][16][17][18][19][20] Sandwich-type ECL immunosensors can achieve satisfactory sensitivity and selectivity. Wang's group designed a sandwich-type ECL immunosensor for determining the CA12-5 level using luminol nanocomposites (AuNPs-EGN-Luminol) labeling Ab 1 as the signal probe and glucose oxidase-encapsulated nanogold hollow microspheres (GOx-GHS) as a sensing platform for fixing Ab 2 .…”

A novel label-free electrochemiluminescence aptasensor using a tetrahedral DNA nanostructure as a scaffold for ultrasensitive detection of organophosphorus pesticides in a luminol–H2O2 system

“…In recent years, researchers have constructed a series of electrochemiluminescence aptasensors that combine the molecular recognition function of aptamers and the signal indication function of electrochemiluminescence. 21,22 Nanomaterials are a class of materials with a size on the nanometer scale, and are extensively used in biosensors due to their large specific surface area, good biocompatibility, and strong electron transferability. Nanomaterials can be used as emitters or can be functionalized with luminol or ruthenium complexes and used as illuminants, such as Au–luminol, 23 PAMAM–Ru(bpy) 3 2+ , 24 and other complexes, producing stable luminous signals under certain conditions.…”

Electrochemiluminescence biosensor for cardiac troponin I with signal amplification based on a MoS2@Cu2O–Ag-modified electrode and Ce:ZnO-NGQDs

“…[6][7][8] Compared with these methods, electrochemiluminescence (ECL) has excellent spatial/temporal control, low background, a large dynamic detection range, an easy optical setup, and great controllability. 9,10 So far, it has been widely used in immunoassays. [11][12][13][14] Our work focuses on building a sandwiched ECL sensor to detect cTnI at ultrasensitive levels.…”