Biofuels for a sustainable future

Liu, Yuzhong; Cruz-Morales, Pablo; Zargar, Amin; Belcher, Michael S.; Pang, Bo; Englund, Elias; Dan, Qingyun; Yin, Kevin; Keasling, Jay D.

doi:10.1016/j.cell.2021.01.052

Cited by 245 publications

(109 citation statements)

References 90 publications

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“…Here, cyanobacteria represent excellent candidates for the expression of plant biosynthetic genes and gene clusters due to their ancestral relationship to plant chloroplasts. In recent years, continuous efforts have been put into developing industrially viable strains of cyanobacteria for the sustainable production of various fine chemicals, secondary metabolites, and other compounds ( Jodlbauer et al, 2021 ; Liu et al, 2021 ). Advances in synthetic microbiology and increasing availability of new genetic tools for this important group of organisms enable even more innovative solutions.…”

Section: Introductionmentioning

confidence: 99%

Metabolic engineering of Synechocystis sp. PCC 6803 for the photoproduction of the sesquiterpene valencene

Dietsch

Behle

Westhoff

et al. 2021

Metabolic Engineering Communications

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Cyanobacteria are extremely adaptable, fast-growing, solar-powered cell factories that, like plants, are able to convert carbon dioxide into sugar and oxygen and thereby produce a large number of important compounds. Due to their unique phototrophy-associated physiological properties, i.e. naturally occurring isoprenoid metabolic pathway, they represent a highly promising platform for terpenoid biosynthesis. Here, we implemented a carefully devised engineering strategy to boost the biosynthesis of commercially attractive plant sequiterpenes, in particular valencene. Sesquiterpenes are a diverse group of bioactive metabolites, mainly produced in higher plants, but with often low concentrations and expensive downstream extraction. In this work we successfully demonstrate a multi-component engineering approach towards the photosynthetic production of valencene in the cyanobacterium Synechocystis sp. PCC 6803. First, we improved the flux towards valencene by markerless genomic deletions of shc and sqs . Secondly, we downregulated the formation of carotenoids, which are essential for viability of the cell, using CRISPRi on crtE . Finally, we intended to increase the spatial proximity of the two enzymes, ispA and CnVS , involved in valencene formation by creating an operon construct, as well as a fusion protein. Combining the most successful strategies resulted in a valencene production of 19 mg/g DCW in Synechocystis . In this work, we have devised a useful platform for future engineering steps.

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Section: Introductionmentioning

confidence: 99%

Metabolic engineering of Synechocystis sp. PCC 6803 for the photoproduction of the sesquiterpene valencene

Dietsch

Behle

Westhoff

et al. 2021

Metabolic Engineering Communications

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show abstract

“…The generation of bioethanol and biodiesel by crops is fully grown. Simultaneously, other supplied resources and methods have also conferred high potential to produce effective and economical substitutes, for example, landfill and plastic garbage regeneration, algal photosynthesis, and electrochemical carbon fixation [160] . Biodiesel is a diesel fuel obtained by plants or animals and consists of long-chain fatty acid esters.…”

Section: Conversion Of Ppe Kits Into a Liquid Biofuelmentioning

confidence: 99%

Key ingredients and recycling strategy of personal protective equipment (PPE): Towards sustainable solution for the COVID-19 like pandemics

Siwal

Chaudhary

Saini

et al. 2021

Journal of Environmental Chemical Engineering

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The COVID-19 pandemic has intensified the complications of plastic trash management and disposal. The current situation of living in fear of transmission of the COVID-19 virus has further transformed our behavioural models, such as regularly using personal protective equipment (PPE) kits and single-use applications for day to day needs etc. It has been estimated that with the passage of the coronavirus epidemic every month, there is expected use of 200 billion pieces of single-use facemasks and gloves. PPE are well established now as life-saving items for medicinal specialists to stay safe through the COVID-19 pandemic. Different processes such as glycolysis, hydrogenation, aminolysis, hydrolysis, pyrolysis, and gasification are now working on finding advanced technologies to transfer waste PPE into value-added products. Here, in this article, we have discussed the recycling strategies of PPE, important components (such as medical gloves, gowns, masks & respirators and other face and eye protection) and the raw materials used in PPE kits. Further, the value addition methods to recycling the PPE kits, chemical & apparatus used in recycling and recycling components into value-added products. Finally, the biorenewable materials in PPE for textiles components have been discussed along with concluded remarks.

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“…Due to the energy crisis caused by the gradual depletion of fossil fuel reserves and the accumulation of greenhouse gases such as carbon dioxide and methane, caused by high consumption of fossil fuels, the demand for alternative renewable energy sources is increasing (Jeswani et al, 2020 ; Kumar et al, 2020 ; Liu et al, 2021 ). Alternative energy sources include solar, wind, geothermal, tidal, and hydroelectric energy along with bioenergy.…”

Section: Introductionmentioning

confidence: 99%

Production of Gloeophyllum trabeum Endoglucanase Cel12A in Nicotiana benthamiana for Cellulose Degradation

et al. 2021

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Lignocellulosic biomass from plants has been used as a biofuel source and the potent acidic endoglucanase GtCel12A has been isolated from Gloeophyllum trabeum, a filamentous fungus. In this study, we established a plant-based platform for the production of active GtCel12A fused to family 3 cellulose-binding module (CBM3). We used the signal sequence of binding immunoglobulin protein (BiP) and the endoplasmic reticulum (ER) retention signal for the accumulation of the produced GtCel12A in the ER. To achieve enhanced enzyme expression, we incorporated the M-domain of the human receptor-type tyrosine-protein phosphatase C into the construct. In addition, to enable the removal of N-terminal domains that are not necessary after protein expression, we further incorporated the cleavage site of Brachypodium distachyon small ubiquitin-like modifier. The GtCel12A-CBM3 fusion protein produced in the leaves of Nicotiana benthamiana exhibited not only high solubility but also efficient endoglucanase activity on the carboxymethyl cellulose substrate as determined by 3,5-dinitrosalicylic acid assay. The endoglucanase activity of GtCel12A-CBM3 was maintained even when immobilized on microcrystalline cellulose beads. Taken together, these results indicate that GtCel12A endoglucanase produced in plants might be used to provide monomeric sugars from lignocellulosic biomass for bioethanol production.

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Biofuels for a sustainable future

Cited by 245 publications

References 90 publications

Metabolic engineering of Synechocystis sp. PCC 6803 for the photoproduction of the sesquiterpene valencene

Metabolic engineering of Synechocystis sp. PCC 6803 for the photoproduction of the sesquiterpene valencene

Key ingredients and recycling strategy of personal protective equipment (PPE): Towards sustainable solution for the COVID-19 like pandemics

Production of Gloeophyllum trabeum Endoglucanase Cel12A in Nicotiana benthamiana for Cellulose Degradation

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