Biofunctional approaches of wool-based keratin for tissue engineering

Ranjit, Eliza; Hamlet, Stephen; George, Roy; Sharma, Ajay Kumar; Love, Robert M.

doi:10.1016/j.jsamd.2021.10.001

Cited by 26 publications

(12 citation statements)

References 124 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has a high mechanical strength due to the peptide bonds, making it suitable for various applications, such as adhesives, fibers, and films. [169] The poultry industry generates a large amount of waste in the form of chicken feather, while the coarse The morphologies of fiber-like unmodified fish scale-derived collagen and methylated collagen. The methylated collagen patch, crosslinked with 1,4-butanediol diglycidyl ether, enabled blood and lymphatic vessel regeneration recognized by the CD31 and LYVE-1 staining around the smooth muscle actin which may be suitable for inflammation-related disease treatment.…”

Section: Keratin From Feathers and Woolsmentioning

confidence: 99%

Sustainable Sources of Raw Materials for Additive Manufacturing of Bone‐Substituting Biomaterials

Putra

Zhou

Zadpoor

2023

Adv Healthcare Materials

View full text Add to dashboard Cite

The need for sustainable development has never been more urgent, as the world continues to struggle with environmental challenges, such as climate change, pollution, and dwindling natural resources. The use of renewable and recycled waste materials as a source of raw materials for biomaterials and tissue engineering is a promising avenue for sustainable development. Although tissue engineering has rapidly developed, the challenges associated with fulfilling the increasing demand for bone substitutes and implants remain unresolved, particularly as the global population ages. This review provides an overview of waste materials, such as eggshells, seashells, fish residues, and agricultural biomass, that can be transformed into biomaterials for bone tissue engineering. While the development of recycled metals is in its early stages, we highlight the use of probiotics and renewable polymers to improve the biofunctionalities of bone implants. Despite the advances of additive manufacturing (AM), studies on AM waste‐derived bone‐substitutes are limited. It is foreseeable that AM technologies can provide a more sustainable alternative to manufacturing biomaterials and implants. The preliminary results of eggshell and seashell‐derived calcium phosphate and rice husk ash‐derived silica will likely pave the way for more advanced applications of AM waste‐derived biomaterials for sustainably addressing several unmet clinical applications.This article is protected by copyright. All rights reserved

show abstract

Section: Keratin From Feathers and Woolsmentioning

confidence: 99%

Sustainable Sources of Raw Materials for Additive Manufacturing of Bone‐Substituting Biomaterials

Putra

Zhou

Zadpoor

2023

Adv Healthcare Materials

View full text Add to dashboard Cite

show abstract

“…Wool fibers consist of 95%−98% proteins (about 80%−85% keratin), lipids (0.1%), and minerals (0.5%; Ranjit et al., 2021; Zhang et al., 2018). Its cross‐section depicts a three‐dimensional hierarchical structure with a covalently attached outer layer of fatty acids lining the exterior cuticular surfaces and β‐layers of the cell membrane complex (Figure 1).…”

Section: Typical Characteristics Of Woolmentioning

confidence: 99%

“…The cuticle layer (epicuticle, exocuticle, and endocuticle), found within the second structural unit, encases a cortex containing highly oriented cortical cells and makes up about 85% of the wool fiber. Generally, wool has three protein classes, (a) the intermediate filament proteins (IFPs), which are low in sulfur; (b) high‐sulfur proteins; and (c) high‐tyrosine proteins (Ferrareze et al., 2016; Ranjit et al., 2021). The cortical cells are organized into high sulfur macro‐ and low sulfur microfibrils (IFP), with the latter making up to 50%−60% w/w of the cortex (Figure 1b).…”

Section: Typical Characteristics Of Woolmentioning

confidence: 99%

Wool keratin as a novel alternative protein: A comprehensive review of extraction, purification, nutrition, safety, and food applications

Giteru

Ramsey

Hou

et al. 2022

Comp Rev Food Sci Food Safe

View full text Add to dashboard Cite

The growing global population and lifestyle changes have increased the demand for specialized diets that require protein and other essential nutrients for humans. Recent technological advances have enabled the use of food bioresources treated as waste as additional sources of alternative proteins. Sheep wool is an inexpensive and readily available bioresource containing 95%–98% protein, making it an outstanding potential source of protein for food and biotechnological applications. The strong structure of wool and its indigestibility are the main hurdles to achieving its potential as an edible protein. Although various methods have been investigated for the hydrolysis of wool into keratin, only a few of these, such as sulfitolysis, oxidation, and enzymatic processes, have the potential to generate edible keratin. In vitro and in vivo cytotoxicity studies reported no cytotoxicity effects of extracted keratin, suggesting its potential for use as a high‐value protein ingredient that supports normal body functions. Keratin has a high cysteine content that can support healthy epithelia, glutathione synthesis, antioxidant functions, and skeletal muscle functions. With the recent spike in new keratin extraction methods, extensive long‐term investigations that examine prolonged exposure of keratin generated from these techniques in animal and human subjects are required to ascertain its safety. Food applications of wool could improve the ecological footprint of sheep farming and unlock the potential of a sustainable protein source that meets demands for ethical production of animal protein.

show abstract

“…Recent studies have demonstrated the ability of wool keratin to support the adhesion and growth of different cell phenotypes including fibroblasts [ 5 ], osteoblasts [ 6 ], neuroblasts and keratinocytes [ 1 , 2 ]. This is strictly related to the presence of cellular binding motifs such as arginine–glycine–aspartic acid (RGD), glutamic acid-aspartic acid-serine (EDS) and leucine–aspartic acid–valine (LVD) that promote the cell attachment, so reproducing the interaction patterns supported by native Extra Cellular Matrix-like proteins [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Wool Keratin Nanofibers for Bioinspired and Sustainable Use in Biomedical Field

Ramírez

Vineis

Cruz‐Maya

et al. 2022

JFB

View full text Add to dashboard Cite

Keratin is a biocompatible and biodegradable protein as the main component of wool and animal hair fibers. Keratin-based materials support fibroblasts and osteoblasts growth. Keratin has been extracted by sulphitolysis, a green method (no harmful chemicals) with a yield of 38–45%. Keratin has been processed into nanofibers from its solutions by electrospinning. Electrospinning is a versatile and easy-to-use technique to generate nanofibers. It is an eco-friendly and economical method for the production of randomly and uniaxially oriented polymeric nanofibers. Thanks to their high specific surface area, nanofibers have great potential in the biomedical field. Keratin nanofibers have received significant attention in biomedical applications, such as tissue engineering and cell growth scaffolds, for their biocompatibility and bio-functionality. Accordingly, we propose an extensive overview of recent studies focused on the optimization of keratinbased nanofibers, emphasizing their peculiar functions for cell interactions and the role of additive phases in blends or composite systems to particularize them as a function of specific applications (i.e., antibacterial).

show abstract

Biofunctional approaches of wool-based keratin for tissue engineering

Cited by 26 publications

References 124 publications

Sustainable Sources of Raw Materials for Additive Manufacturing of Bone‐Substituting Biomaterials

Sustainable Sources of Raw Materials for Additive Manufacturing of Bone‐Substituting Biomaterials

Wool keratin as a novel alternative protein: A comprehensive review of extraction, purification, nutrition, safety, and food applications

Wool Keratin Nanofibers for Bioinspired and Sustainable Use in Biomedical Field

Contact Info

Product

Resources

About