Biofunctional polyethylene glycol coatings on titanium: An in vitro-based comparison of functionalization methods

Buxadera‐Palomero, Judit; Calvo, Cristina; Torrent-Camarero, Sergi; Gil, F.J.; Mas‐Moruno, Carlos; Canal, Cristina; Rodríguez, Daniel

doi:10.1016/j.colsurfb.2017.01.042

Cited by 57 publications

(46 citation statements)

References 82 publications

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“…67 Similar ndings have been reported in related studies on various bacterial strains. [68][69][70] Our ndings are consistent with those in previous reports. The extent of bacterial adhesion on PDA-modied surfaces with or without immobilized RGD was signicantly lower than that on pristine zirconia, and all modications were similarly effective in reducing bacterial colonization; thus, the antibacterial effect was presumably largely contributed by PDA.…”

Section: Bacterial Responsessupporting

confidence: 93%

Biofunctionalization of zirconia with cell-adhesion peptides via polydopamine crosslinking for soft tissue engineering: effects on the biological behaviors of human gingival fibroblasts and oral bacteria

et al. 2020

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Rapid soft tissue integration is essential for long-term dental implant success. Zirconia is increasingly used as an abutment material owing to its excellent aesthetic properties and biocompatibility; however, it is bioinert, and tissue integration is poor. We developed a feasible surface modification method, exploiting the reactivity of polydopamine (PDA) films to immobilize cell-adhesion peptides (Arg-Gly-Asp, RGD) onto zirconia abutment surfaces. Further, we evaluated the effect thereof on human gingival fibroblast (HGF) behavior and oral bacterial adhesion, which influence the peri-implant soft tissue seal. HGF responses to linear KGGRGDSP and cyclic RGDfK sequences were compared. PDA deposition and covalent coupling of RGD were verified by X-ray photoelectron spectroscopy and fluorescence microscopy. The biological behaviors of HGFs on the modified zirconia; i.e., adhesion, spreading, proliferation, gene and protein expression, were elucidated. Biofunctionalization of zirconia with the adhesion peptides significantly enhanced the biological activities of HGFs. Cyclic RGD induced slightly improved cell attachment, spreading, and proliferation, but similar cell differentiation when compared to linear RGD peptides. To assess their antimicrobial properties, the different substrates were exposed to cultures of the early colonizer Streptococcus mutans or the periodontal pathogen Porphyromonas gingivalis, and bacterial adhesion was evaluated by scanning electron microscopy and live/dead staining.PDA and PDA-RGD coatings decreased zirconia surface colonization by both bacterial species to similar extents. Thus, PDA-RGD-functionalized zirconia modulates specific HGF responses, while maintaining the antimicrobial activity of the PDA coating. The selective bio-interaction pattern of this surface modification holds great promise for improving soft-tissue integration around zirconia abutments in clinical applications.

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Section: Bacterial Responsessupporting

confidence: 93%

Biofunctionalization of zirconia with cell-adhesion peptides via polydopamine crosslinking for soft tissue engineering: effects on the biological behaviors of human gingival fibroblasts and oral bacteria

et al. 2020

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“…However, the authors did not check the response of eukaryotic cells to the RGD peptide on these studies. Both effects were actually reported in other investigations, which reflected the multifunctional potential of this strategy: the passive PEG layer inhibited bacterial attachment, while the RGD peptide simultaneously supported (or improved) osteoblast (OB) or fibroblast (FB) adhesion. In addition to electrostatic adsorption, a number of other methods have been proposed to coat Ti surfaces with PEG, such as electrodeposition, silanization, and plasma polymerization …”

Section: Multifunctional Chemical Coatingssupporting

confidence: 70%

Multifunctional Coatings and Nanotopographies: Toward Cell Instructive and Antibacterial Implants

Mas‐Moruno

Dalby

2018

Adv Healthcare Materials

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188

171

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In biomaterials science, it is nowadays well accepted that improving the biointegration of dental and orthopedic implants with surrounding tissues is a major goal. However, implant surfaces that support osteointegration may also favor colonization of bacterial cells. Infection of biomaterials and subsequent biofilm formation can have devastating effects and reduce patient quality of life, representing an emerging concern in healthcare. Conversely, efforts toward inhibiting bacterial colonization may impair biomaterial–tissue integration. Therefore, to improve the long‐term success of medical implants, biomaterial surfaces should ideally discourage the attachment of bacteria without affecting eukaryotic cell functions. However, most current strategies seldom investigate a combined goal. This work reviews recent strategies of surface modification to simultaneously address implant biointegration while mitigating bacterial infections. To this end, two emerging solutions are considered, multifunctional chemical coatings and nanotopographical features.

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“…In contrast, the PEG coatings drastically reduce the amount of protein attachment, in agreement with their well-known antifouling properties [10,42]. It is important to highlight that the low fouling behavior of PEGylated surfaces was maintained after the PTF immobilization, thus indicating that neither the RGD sequence, nor the LF1-11 peptide promote any non-specific protein attachment.…”

Section: Protein Adsorptionsupporting

confidence: 65%

“…To this end, we propose a non-time consuming and straightforward method based on the electrodeposition of PEG on titanium [40][41][42] and subsequent immobilization of a peptidic platform (PTF) previously developed by us [36], which simultaneously presents the two peptide sequences (RGD + LF1-11). The positive effects of the RGD integrin binding peptide in promoting cell adhesion on biomaterials are well established [43,44].…”

Section: Introductionmentioning

confidence: 99%

All-in-one trifunctional strategy: A cell adhesive, bacteriostatic and bactericidal coating for titanium implants

Hoyos‐Nogués

Buxadera‐Palomero

Ginebra

et al. 2018

Colloids and Surfaces B: Biointerfaces

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Strategies to inhibit initial bacterial adhesion are extremely important to prevent infection on biomaterial surfaces. However, the simultaneous attraction of desired eukaryotic cells remains a challenge for successful biomaterial-host tissue integration. Here we describe a method for the development of a trifunctional coating that repels contaminating bacteria, kills those that adhere, and promotes osteoblast adhesion. To this end, titanium surfaces were functionalized by electrodeposition of an antifouling polyethylene glycol (PEG) layer and subsequent binding of a peptidic platform with cell-adhesive and bactericidal properties. The physicochemical characterization of the samples via SEM, contact angle, FTIR and XPS analysis verified the successful binding of the PEG layer and the biomolecules, without altering the morphology and topography of the samples. PEG coatings inhibited protein adsorption and osteoblast-like (SaOS-2) attachment; however, the presence of cell adhesive domains rescued osteoblast adhesion, yielding higher values of cell attachment and spreading compared to controls (p < 0.05). Finally, the antibacterial potential of the coating was measured by live/dead assays and SEM using S. sanguinis as a model of early colonizer in oral biofilms. The presence of PEG layers significantly reduced bacterial attachment on the surfaces (p < 0.05). This antibacterial potential was further increased by the bactericidal peptide, yielding values of bacterial adhesion below 0.2% (p < 0.05). The balance between the risk of infection and the optimal osteointegration of a biomaterial is often described as "the race for the surface", in which contaminating bacteria and host tissue cells compete to colonize the implant. In the present work, we have developed a multifunctional coating for a titanium surface that promotes the attachment and spreading of osteoblasts, while very efficiently inhibits bacterial colonization, thus holding promise for application in bone replacing applications.

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Biofunctional polyethylene glycol coatings on titanium: An in vitro-based comparison of functionalization methods

Cited by 57 publications

References 82 publications

Biofunctionalization of zirconia with cell-adhesion peptides via polydopamine crosslinking for soft tissue engineering: effects on the biological behaviors of human gingival fibroblasts and oral bacteria

Biofunctionalization of zirconia with cell-adhesion peptides via polydopamine crosslinking for soft tissue engineering: effects on the biological behaviors of human gingival fibroblasts and oral bacteria

Multifunctional Coatings and Nanotopographies: Toward Cell Instructive and Antibacterial Implants

All-in-one trifunctional strategy: A cell adhesive, bacteriostatic and bactericidal coating for titanium implants

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