Biofunctional Surfaces

Superparamagnetic Fe3O4 Nanoparticles: Synthesis by Thermal Decomposition of Iron(III) Glucuronate and Application in Magnetic Resonance Imaging

“…Advantage of carbohydrate coatings of Fe 3 O 4 consists in their good biocompatibility and better cellular internalization in contrast to the widely used PEG. Moreover, compared to another commonly applied silica shell, carbohydrates prevent undesirable interactions with proteins reducing thus opsonization and prolonging particle serum and blood half-life that is important for in vitro and in vivo applications . The hydrophilic PMG–P ligand was synthesized according to Scheme .…”

Engineering Biocompatible Interfaces via Combinations of Oxide Films and Organic Self-Assembled Monolayers

“…They are ultrathin (monolayer) and, due to their chemical binding to the substrate, quite robust . Since they allow the physical and chemical properties of the substrate surface to be tailored in many ways, SAMs in combination with soft-lithographical methods are often used nowadays for guided cell experiments . In order to achieve a large cell adhesion contrast, normally, backfill chemistry is needed to block the unwanted docking sites for cells.…”

Novel biomedical applications of supported lipid bilayers

“…Cushioned SLBs have been developed to position the SLB further away from the support as reviewed by Tanaka and Sackmann 58 and more recently by Knoll and co-workers. 52,53,[59][60][61] Cushioned SLBs are better suited to accommodate large transmembrane proteins since the increased distance above the substrate avoids frictional coupling between the SLB and the substrate when the transmembrane proteins are engaged with other biomolecules. Cremer and co-workers proposed doubly cushioned architectures using PEGylated SLBs and passivated glass supports.…”

“…52,53,[59][60][61]212 For instance, SLBs formed on a porous nanoarray doped with the gramicidin-D ion channel could be measured using impedance spectroscopy. 213 More recently, arrays of free standing SLBs have been used to monitor active and passive transport across α-hemolysin (Figure 1.12c) and F 0 F 1 -ATP synthase, respectively.…”

“…109 Electrical analysis of SLBs with or without embedded membrane proteins has been shown in particular with tethered SLB architectures, which is reviewed by Knoll and coworkers. 52,53,61 Scanning probe techniques such as AFM can be used to measure the thickness of SLBs [110][111][112] and the rigidity of pore spanning SLBs and to map the surface topography to, for example, visualize lipid rafts (Figure 1.7d). 96,113 The use of AFM in studying phase transitions in SLBs have recently been reviewed.…”