Biofunctional Surfaces Based on Dendronized Cellulose

Abstract

Biofunctionalized surfaces based on dendronized cellulose were prepared either by embedding 6-deoxy-6-(1,2,3-triazolo)-4-polyamidoamine (PAMAM) cellulose (degree of substitution, DS 0.25), obtained by homogeneous conversion of 6-deoxy-6-azido cellulose with propargyl-PAMAM dendron via the copper-catalyzed Huisgen reaction, in a cellulose acetate (DS 2.50) matrix or by the heterogeneous functionalization of deoxy-azido cellulose film with the dendron. The amount of amino groups provided by the solid supports was determined and the covalent attachment of enzyme was proven with glucose oxidase as model enzyme after activation with glutardialdehyde. The quality of glucose oxidase immobilization was defined by determining of the specific enzyme activity, coupling efficiency, storage stability, and reproducibility. Although the heterogeneous functionalization of the deoxy-azido film yields a product that binds more enzyme compared to the blend of dendronized cellulose derivative with cellulose acetate, the coupling efficiency is comparatively small. Nevertheless, the different approaches for the preparation of biofunctionalized surfaces based on dendronized cellulose provide an excellent reproducibility and good storage stability.

Synthesis and characterization of cellulose fibers grafted with hyperbranched poly(3-methyl-3-oxetanemethanol)

“…The dendronized celluloses possess a wide range of solubilities in organic solvents. Similarly, Heinze and Pohl et al dendronized cellulose with polyester and polyamidoamine dendrons (Heinze et al 2007;Heinze et al 2008;Hwang et al 2006;Pohl et al 2009) to make it soluble in polar solvents. In addition to enhancing solubility of cellulose, the dendronization can also endow cellulose novel properties and applications.…”

Designing syntheses of cellulose and starch derivatives with basic or cationic N‐functions: part I—cellulose derivatives

“…Another promising approach for synthesis of cationic or basic cellulose derivatives having N ‐functions is the introduction of an appropriate moiety (azide or carbon–carbon triple bond) into cellulose by S N reaction and subsequent Huisgen cycloaddition as a click reaction . Huisgen reaction is a 1,3‐dipolar cycloaddition between an azide and a terminal or internal alkyne to give a 1,2,3‐triazole.…”

Preparation of reactive fibre interfaces using multifunctional cellulose derivatives

“…A colorimetric method using Acid Orange 7 dye ("AO7") was used for the quantitative determination of the amino groups from amino-fibres (Pohl et al, 2009). The method relies on the formation of ion pairs from the negatively charged sulfonate groups from AO7 with the protonated primary amino groups from the amino-fibres.…”