Biofunctionalization of porcine-derived collagen matrices with platelet rich fibrin: influence on angiogenesis in vitro and in vivo

Blatt, Sebastian; Burkhardt, Valentin; Kämmerer, Peer W.; Pabst, Andreas; Sagheb, Keyvan; Heller, Martin; Al‐Nawas, Bilal; Schiegnitz, Eik

doi:10.1007/s00784-020-03213-8

Cited by 38 publications

(68 citation statements)

References 58 publications

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“…A vacutainer system and specific sterile plain vacuum tubes with additional silicone within their coating surface were, respectively, used for solid (A-PRF+, Mectron, Carasco, Italy) and liquid PRF (iPRF, Mectron, Carasco, Italy). PRF was directly manufactured with a fixed angle rotor with a radius of 110 mm at 1200 rpm and a relative centrifugal force of 177 g for 8 min (Duo centrifuge, Mectron, Carasco, Italy) following the manufacturer’s instructions [ 7 ].…”

Section: Methodsmentioning

confidence: 99%

“…In contrast, autologous platelet concentrates such as platelet-rich fibrin (PRF) that are now broadly used in dental and craniomaxillofacial regenerative medicine may potentially overcome this limitation [ 5 ]. Thus far, the pro-angiogenic effect of the PRF has been demonstrated to mainly impact soft tissue regeneration procedures [ 6 , 7 ], but the complex interplay of different cytokines and growth factors leads to an increased proliferation and differentiation of different cell lines, inter alia, osteoblasts [ 8 , 9 ]. In detail, vascular endothelial growth factor (VEGF), tissue growth factor ß (TGFß), and platelet-derived growth factor (PDGF) have been discussed to trigger vasoformative responses [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…Recently, our working group showed a positive effect of PRF in combination with allogeneic and xenogeneic BSMs that enhanced osteoblast activity compared to a native BSM in vitro at later time points [ 7 ]. Thus far, no comparative study has analyzed the early angiogenetic interactions of PRF with different BSMs in vitro nor translated these results to in vivo experiments.…”

Section: Introductionmentioning

confidence: 99%

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Does Platelet-Rich Fibrin Enhance the Early Angiogenetic Potential of Different Bone Substitute Materials? An In Vitro and In Vivo Analysis

et al. 2021

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The impaired angiogenic potential of bone substitute materials (BSMs) may limit regenerative processes. Therefore, changes in the angiogenetic properties of different BSMs in combination with platelet-rich fibrin (PRF) in comparison to PRF alone, as well as to native BSMs, were analyzed in vitro and in vivo to evaluate possible clinical application. In vitro, four BSMs of different origins (allogeneic, alloplastic, and xenogeneic) were biofunctionalized with PRF and compared to PRF in terms of platelet interaction and growth factor release (vascular endothelial growth factor (VEGF), tissue growth factor ß (TGFß) and platelet-derived growth factor (PDGF)) after 15 min. To visualize initial cell–cell interactions, SEM was performed. In vivo, all BSMs (±PRF) were analyzed after 24 h for new-formed vessels using a chorioallantoic membrane (CAM) assay. Especially for alloplastic BSMs, the addition of PRF led to a significant consumption of platelets (p = 0.05). PDGF expression significantly decreased in comparison to PRF alone (all BSMs: p < 0.013). SEM showed the close spatial relation of each BSM and PRF. In vivo, PRF had a significant positive pro-angiogenic influence in combination with alloplastic (p = 0.007) and xenogeneic materials (p = 0.015) in comparison to the native BSMs. For bio-activated xenogeneic BSMs, the branching points were also significantly increased (p = 0.005). Finally, vessel formation was increased for BSMs and PRF in comparison to the native control (allogeneic: p = 0.046; alloplastic: p = 0.046; and xenogeneic: p = 0.050). An early enhancement of angiogenetic properties was demonstrated when combining BSMs with PRF in vitro and led to upregulated vessel formation in vivo. Thus, the use of BSMs in combination with PRF may trigger bony regeneration in clinical approaches.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Does Platelet-Rich Fibrin Enhance the Early Angiogenetic Potential of Different Bone Substitute Materials? An In Vitro and In Vivo Analysis

et al. 2021

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“…These membranes mostly consist of porcine or bovine collagen and are characterized by a good barrier function [3,6]. As a biomaterial, collagen has a number of properties including low immunogenicity, fast vascularization, promotion of wound healing and even bone regeneration [7][8][9][10][11]. It was shown that the material allows sufficient diffusion of nutrients for cellular proliferation and differentiation [12].…”

Section: Introductionmentioning

confidence: 99%

A collagen membrane influences bone turnover marker in vivo after bone augmentation with xenogenic bone

et al. 2020

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Background The aim was to compare early biochemical and histological osseous healing of chronic mandibular defects regenerated with bovine bone substitute with and without collagen membrane in vivo. Methods Eight weeks after formation of a lateral full-thickness perforating bone defect in the mandible of 40 rabbits, bovine bone substitute with (“+”;n = 20) and without (“-”;n = 20) collagen membrane was applied. Blood and bone was collected 24, 72 h, 7, 14 and 21 days after surgery. Total acid phosphatase, bone acid phosphatase, total alkaline phosphatase and bone alkaline phosphatase activities were compared between groups. Formation of new bone was quantified histologically for all time points. Results Twenty-four hours after surgery, bone alkaline phosphatase was significantly elevated in “+” group when compared to “-” (p=0.012). After 72 hours, all bone turnover markers except for total acid phosphatase (p=0.078) where significantly elevated in “+” (all p < 0.05). Fourteen days after surgery, the significant highest values for all bone turnover markers were detected in “-” (all p < 0.05). A significant difference in favor of group “-” could also be detected after 3 weeks in terms of both acid phosphatases (p < 0.05). In histology, no significant differences could be detected. Conclusion Bone regeneration with bovine bone substitute material and collagen membrane shows a significantly earlier bone remodeling activity but does not seem to influence formation of new bone in histological samples.

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“…These membranes mostly consist of porcine or bovine collagen and are characterized by a good barrier function [3,6]. As a biomaterial, collagen has a number of properties including low immunogenicity, fast vascularization, promotion of wound healing and even bone regeneration [7][8][9][10][11]. It was shown that the material allows su cient diffusion of nutrients for cellular proliferation and differentiation [12].…”

Section: Introductionmentioning

confidence: 99%

A Collagen Membrane Influences Bone Turnover Marker in Vivo After Bone Augmentation With Xenogenic Bone

Staedt

Dau

Schiegnitz

et al. 2020

Preprint

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Background:The aim was to compare early biochemical and histological osseous healing of chronic mandibular defects regenerated with bovine bone substitute with and without collagen membranein vivo. Methods:Eight weeks after formation of a lateral defect in the mandible of 40 rabbits, bovine bone substitute with (“+”;n=20) and without (“-“;n=20) collagen membrane was applied. Blood and bone was collected 24, 72 hours, 7, 14 and 21 days after surgery. Total acid phosphatase, bone acid phosphatase, total alkaline phosphatase and bone alkaline phosphatase activities were compared between groups. Formation of new bone was quantified histologically for all time points.Results:24 hours after surgery, bone alkaline phosphatase was significantly elevated in “+” group when compared to “-“ (p=0.012). After 72 hours, all bone turnover markers except for total acid phosphatase (p=0.078) where significantly elevated in “+” (all p<0.05). 14 days after surgery, the significant highest values for all bone turnover markers were detected in “-“ (all p<0.05). A significant difference in favor of group “-“ could also be detected after 3 weeks in terms of both acid phosphatases (p<0.05). In histology, no significant differences could be detected.Conclusion: Bone regeneration with bovine bone substitute material and collagen membrane shows a significantly earlier bone remodeling activity but does not seem to influence formation of new bone in histological samples.

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Biofunctionalization of porcine-derived collagen matrices with platelet rich fibrin: influence on angiogenesis in vitro and in vivo

Cited by 38 publications

References 58 publications

Does Platelet-Rich Fibrin Enhance the Early Angiogenetic Potential of Different Bone Substitute Materials? An In Vitro and In Vivo Analysis

Does Platelet-Rich Fibrin Enhance the Early Angiogenetic Potential of Different Bone Substitute Materials? An In Vitro and In Vivo Analysis

A collagen membrane influences bone turnover marker in vivo after bone augmentation with xenogenic bone

A Collagen Membrane Influences Bone Turnover Marker in Vivo After Bone Augmentation With Xenogenic Bone

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