Biofunctionalization of surfaces of nanostructured porous silicon

Arroyo-Hernández, María; Martín‐Palma, Raúl J.; Pérez‐Rigueiro, José; García–Ruiz, Josefa P.; García-Fierro, José Luis; Martı́nez-Duart, J.M.

doi:10.1016/j.msec.2003.09.159

Cited by 64 publications

(43 citation statements)

References 14 publications

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“…Currently, thermal oxidation is the most popular stabilization process for PSi. Whereas such stabilization is crucial for optoelectronic technologies, its necessity for biological applications is yet uncertain [15][16][17][18][19]. In particular, it is unclear whether the direct derivatization of a fresh PSi surface is adequate for subsequent biological experiments.…”

Section: Introductionmentioning

confidence: 99%

Chemical stabilization of porous silicon for enhanced biofunctionalization with immunoglobulin

Naveas

Costa

Gallach

et al. 2012

Science and Technology of Advanced Materials

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Porous silicon (PSi) is widely used in biological experiments, owing to its biocompatibility and well-established fabrication methods that allow tailoring its surface. Nevertheless, there are some unresolved issues such as deciding whether the stabilization of PSi is necessary for its biological applications and evaluating the effects of PSi stabilization on the surface biofunctionalization with proteins. In this work we demonstrate that non-stabilized PSi is prone to detachment owing to the stress induced upon biomolecular adsorption. Biofunctionalized non-stabilized PSi loses the interference properties characteristic of a thin film, and groove-like structures resulting from a final layer collapse were observed by scanning electron microscopy. Likewise, direct PSi derivatization with 3-aminopropyl-triethoxysilane (APTS) does not stabilize PSi against immunoglobulin biofunctionalization. To overcome this problem, we developed a simple chemical process of stabilizing PSi (CoxPSi) for biological applications, which has several advantages over thermal stabilization (ToxPSi). The process consists of chemical oxidation in H 2 O 2 , surface derivatization with APTS and a curing step at 120• C. This process offers integral homogeneous PSi morphology, hydrophilic surface termination (contact angle θ = 26• ) and highly efficient derivatized and biofunctionalized PSi surfaces (six times more efficient than ToxPSi). All these features are highly desirable for biological applications, such as biosensing, where our results can be used for the design and optimization of the biomolecular immobilization cascade on PSi surfaces.

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Section: Introductionmentioning

confidence: 99%

Chemical stabilization of porous silicon for enhanced biofunctionalization with immunoglobulin

Naveas

Costa

Gallach

et al. 2012

Science and Technology of Advanced Materials

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“…Potential applications include dye-sensitized solar cells [8][9][10], low-k dielectric materials [11,12], photocatalysis [13,14], biosensors [15][16][17], optoelectronics [18][19][20], and antireflecting and self-cleaning coatings [21], to name a few. In these applications, predicting the effects of porosity and pore shape, size, and spatial arrangement on the optical and dielectric properties is essential to the design of mesoporous materials with desired performances or for material characterization purposes.…”

Section: Introductionmentioning

confidence: 99%

Effective optical properties of highly ordered mesoporous thin films

et al. 2010

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This paper expands our previous numerical studies predicting the optical properties of highly ordered mesoporous thin films from two-dimensional (2D) nanostructures with cylindrical pores to three-dimensional (3D) structures with spherical pores. Simple, face centered, and body centered cubic lattice of spherical pores and hexagonal lattice of cylindrical pores were considered along with various pore diameter and porosity. The transmittance and reflectance were numerically computed by solving 3D Maxwell's equations for transverse electric and transverse magnetic polarized waves normally incident on the mesoporous thin films. The effective optical properties of the films were determined by an inverse method. Reflectance of 3D cubic mesoporous thin films was found to be independent of polarization, pore diameter, and film morphology and depended only on film thickness and porosity. By contrast, reflectance of 2D hexagonal mesoporous films with cylindrical pores depended on pore shape and polarization. The unpolarized reflectance of 2D hexagonal mesoporous films with cylindrical pores was identical to that of 3D cubic mesoporous films with the same porosity and thickness. The effective refractive and absorption indices of 3D films show good agreement with predictions by the 3D Maxwell-Garnett and Nonsymmetric Bruggeman effective medium approximations, respectively.

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“…In those bio-applications, surface modification of particles is essential for the immobilization of biomolecules [1][2][3][4]. In particular, amination is known as a typical surface modification method and deeply related to the linkage between surface and target biomolecules such as antibodies, antigen, enzymes, RNA, and DNA [5][6]. Moreover, there have been many reports on the application of amino groups attached to various surfaces to biosensors, patterning, biological modeling and sensing, and coatings, etc [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

“…Generally, surface amination has been achieved on the surface of nanostructured materials synthesized by various processes such as sol-gel, hydrothermal and chemical vapor deposition (CVD) [5][6][8][9][10]. However, since such methods are separated from the amination step, long processing time and complicated process lay-out have been considered as the challenges to overcome for better production of bio-functionalized surfaces.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous amination of TiO₂nanoparticles in the gas phase synthesis for bio-medical applications

Lee

Kim

Lee

et al. 2011

IOP Conf. Ser.: Mater. Sci. Eng.

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Biofunctionalization of surfaces of nanostructured porous silicon

Cited by 64 publications

References 14 publications

Chemical stabilization of porous silicon for enhanced biofunctionalization with immunoglobulin

Chemical stabilization of porous silicon for enhanced biofunctionalization with immunoglobulin

Effective optical properties of highly ordered mesoporous thin films

Simultaneous amination of TiO₂nanoparticles in the gas phase synthesis for bio-medical applications

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Biofunctionalization of surfaces of nanostructured porous silicon

Cited by 64 publications

References 14 publications

Chemical stabilization of porous silicon for enhanced biofunctionalization with immunoglobulin

Chemical stabilization of porous silicon for enhanced biofunctionalization with immunoglobulin

Effective optical properties of highly ordered mesoporous thin films

Simultaneous amination of TiO2nanoparticles in the gas phase synthesis for bio-medical applications

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Simultaneous amination of TiO₂nanoparticles in the gas phase synthesis for bio-medical applications